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Biomedical subjects

R Rappuoli

Publications and source records attributed to R Rappuoli.

298 records · Page 17Linked to original sources
PubMed1982

Production and characterization of high titre rabbit antigonococcal R-type lipopolysaccharide serum.

Two methods are reported for the production of rabbit anti-gonococcal lipopolysaccharide sera. One is produced by a conjugate between the core oligosaccharide and bovine serum albumin. The other is obtained by immunizing rabbits with heat-killed, ethanol-acetone washed bacteria. The specificity of the latter serum is studied, and it is shown that most of the antibodies are against a lactose-like determinant situated in the core region of the lipopolysaccharide. Preliminary studies show that this serum is able to cause specific agglutination of gonococci.

Agglutination Tests↗
PubMed1981

Restriction map of corynebacteriophages beta c and beta vir and physical localization of the diphtheria tox operon.

The BamHI, EcoRI, HindIII, and KpnI restriction endonuclease maps of corynebacteriophage beta c and beta vir were constructed. beta vir appeared to be identical to beta c, except for an approximate 1-kilobase deletion that removed a BamHI site, two KpnI sites, and three EcoRI restriction sites. The diphtheria tox operon was located by hybridizing in vitro 32P-labeled tox messenger ribonucleic acid to blots of endonuclease-digested beta deoxyribonucleic acids. The messenger ribonucleic acid probe was found to hybridize to a 2.1-kilobase region of the beta genome. Since approximately 1.9 kilobases is required to encode prodiphtheria toxin, the data presented strongly suggest that the tox operon of beta is monocistronic.

Bacteriophages↗
PubMedDate not supplied

Differences in the immunogenicity of native and formalinized cross reacting material (CRM197) of diphtheria toxin in mice and guinea pigs and their implications on the development and control of diphtheria vaccine based on CRMs.

Immunogenicity of native and formalinized cross reacting material (CRM197) of diphtheria toxin (DTx) was assessed in mice and guinea pigs. For the primary response, mice produced similar levels of diphtheria toxoid (DTxd) IgG antibodies to both native and formalinized preparations of CRM197 though the antibody levels were significantly lower than those elicited by conventional DTxd (P < 0.05). In contrast, guinea pigs showed significantly higher levels of DTxd IgG antibodies to the formalinized CRM197 preparation than the native preparation (P < 0.001) after single injection. These differences in the immunogenicity of mice and guinea pigs to native and formalinized CRM197 preparations have implications for the development and control of diphtheria or other vaccines involving the use of CRMs.

Animals↗
PubMedDate not supplied

Purification of gonococcal R-type lipopolysaccharide not bearing the lactose-like antigen.

The gonococcal strain R-10 is shown to contain two antigenically different R-type lipopolysaccharides: one of these has the same properties described for the gonococcal lipopolysaccharide, while the other does not have any cross-reaction with it. The latter was purified from a mutated colony able to express only one type of lipopolysaccharide. By inhibition enzyme-linked immunosorbent assay it was shown that the difference between the two lipopolysaccharides is located in the core region which, when purified and analyzed by gel filtration and gas liquid chromatography, did not show any difference either in size or in chemical composition, leading to the conclusion that the two cores must differ just in the structure of the antigenic determinant.

Animals↗