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Biomedical subjects

R Quigley

Publications and source records attributed to R Quigley.

43 records · Page 3Linked to original sources
PubMed1988

Synthesis and biological activity of an azido derivative of paclobutrazol, an inhibitor of gibberellin biosynthesis.

A photolabile azido derivative of the kaurene oxidase inhibitor 1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-l-yl) pentan-3-ol (paclobutrazol) has been synthesized for use as a photoaffinity labeling agent. The compound was tested as an inhibitor of the oxidation of ent-kaurene catalyzed by cell-free preparations from endosperm of Cucurbita maxima. The I(50) of the azido derivative was 9.5 nanomolar, which compares well with that of paclobutrazol (6.3 nanomolar in our measurements). The azido compound bound to Cytochrome P-450 in microsomes from Cucurbita maxima, and induced a Type II spectral change, with an apparent binding constant of 0.24+/-0.04 micromolar.

Journal Article↗
PubMedDate not supplied

Delayed methotrexate clearance in a patient with sickle cell anemia and osteosarcoma.

A 15-year-old girl with homozygous sickle cell anemia (HbSS) and osteosarcoma is described. Delayed clearance of methotrexate (MTX) after the second course of high-dose MTX (HDMTX) led to the development of renal and hepatic toxicities. Rescue was accomplished with high-dose leucovorin, intravenous carboxypeptidase G2, and thymidine. Although the renal and hepatic abnormalities resolved, focal tonic-clonic seizures developed, accompanied by abnormal brain imaging. Four weeks after this episode, all clinical and biochemical abnormalities resolved. Preexistent end-organ damage associated with HbSS may compromise the ability to deliver high-dose chemotherapy with curative intent in patients with malignant disease.

Adolescent↗
PubMedDate not supplied

Proximal renal tubular acidosis.

The proximal tubule reabsorbs approximately 80% of the filtered load of bicarbonate. Defects in the process of bicarbonate reabsorption result in loss of bicarbonate and proximal renal tubular acidosis. Global proximal tubule dysfunction is known as the Fanconi's syndrome. Both isolated proximal renal tubular acidosis and the Fanconi's syndrome can result from inherited defects or can be acquired. This review will discuss the mechanisms that cause defects in proximal tubule bicarbonate transport as well as the common causes of isolated proximal renal tubular acidosis and the Fanconi syndrome.

Acidosis, Renal Tubular↗
PubMedDate not supplied

Determinants of influenza vaccination uptake among hospital healthcare workers.

Influenza vaccination of health care workers is recommended for their own protection and that of their patients. There is serendipitous protection for their contacts outside of work. Influenza vaccination uptake has been historically poor. A questionnaire study of healthcare staff at a tertiary referral hospital was carried out to evaluate knowledge of and attitudes to influenza and its vaccine. There was a 36% response rate to the questionnaire, but with reasonably proportionate representation of the various occupational groups. The mean acceptance of vaccine in respondents was 30%; however the vaccination rate for the hospital in 2003 was 16.2% indicating that non-vaccinees were under represented in the study sample.. Vaccine uptake increased with age. Occupationally, doctors, clerical and catering/household staff had higher than mean reported uptake of vaccine. Nurse respondents reported vaccine uptake of 22%. The role of these factors is examined in determining vaccine acceptance. A strong belief in the effectiveness of influenza vaccine was shown to be the strongest predictor of vaccine uptake with 66% of this group taking vaccine. A maximum score in an 8-question knowledge test was only associated with a 51% vaccine uptake. Implications for campaigns to improve uptake are discussed.

Adolescent↗
PubMedDate not supplied

Clearance of ticarcillin-clavulanic acid by continuous venovenous hemofiltration in three critically ill children, two with and one without concomitant extracorporeal membrane oxygenation.

Three children were receiving ticarcillin-clavulanic acid by continuous venovenous hemofiltration (CVVH). Two of them were also receiving concomitant extracorporeal membrane oxygenation (ECMO). We collected ultrafiltrate hourly to determine the clearance of ticarcillin-clavulanic acid by CVVH. Serum concentrations were also determined at the midpoint of each ultrafiltrate collection. All samples were collected over one dosing interval. The volume of distribution of ticarcillin and clavulanic acid was 0.26 +/- 0.01 and 0.69 +/- 0.23 L/kg, respectively. Total body clearance of ticarcillin, determined from the elimination rate constant and volume of distribution, was 0.038 +/- 0.003 L/kg/hour and for clavulanic acid was 0.18 +/- 0.03 L/kg/hour. The sieving coefficients for ticarcillin and clavulanic acid were 0.83 +/- 0.11 and 1.69 +/- 0.19, respectively. We attempted to estimate the clearances by ECMO, but the result was uninterpretable.

Adolescent↗