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Biomedical subjects

R Pandey

Publications and source records attributed to R Pandey.

At least 127 records · Page 7Linked to original sources
PubMed1990

Effects of spontaneous and simulated gastroesophageal reflux on sleeping asthmatics.

We examined the effect of intraesophageal acid (either spontaneous gastroesophageal reflux or infused) on airflow resistance in 15 sleeping asthmatic subjects. We observed no significant acute or sustained changes in airflow resistance relative to periods of intraesophageal acid. Overnight changes in spirometry and lower airway resistance also demonstrated similar nocturnal worsening of bronchoconstriction despite the occurrence of spontaneous or simulated gastroesophageal reflux. The presence or absence of clinical evidence of esophagitis (Bernstein test response) did not alter the observed lack of response to intraesophageal acid. We conclude that gastroesophageal reflux contributes little to the nocturnal worsening of asthma.

Adult↗
PubMed1990

Cell culture propagation of calf rotavirus and detection of rotavirus specific antibody in colostrum and milk of cows and buffaloes.

A bovine rotavirus was adapted to serial propagation in Madin Darby bovine kidney (MDBK) cells. Virus of cell culture origin when examined by electron microscopy exhibited characteristic rotavirus morphology and had a typical RNA electropherotype on polyacrylamide gel electrophoresis. Mean IgG concentration in the first colostral whey was 62.80 +/- 6.56 mg/ml in cows and 104.40 +/- 31.12 mg/ml and 2.52 +/- 0.28 mg/ml respectively. The geometric mean neutralising antibody titre against bovine rotavirus in the first colostral whey was 279 in cows and 139 in buffaloes. It declined to 10 and 13 (respectively) by the tenth day.

Animals↗
PubMed1989

Effect of pinealectomy on annual testicular cycle of Indian chequered water snake, Natrix piscator.

Pinealectomy for 60 days (noted every month of the year), in the tropical Indian chequered water snake. Natrix piscator, caused inhibition of testicular growth during late progressive and active phases and retarded the rate of regression during regressive phase; during sexually inactive phase the testes remain partially active. The weight of the accessory sex organs, vas deferens, and kidney (along with the renal sex segment activity) followed the same pattern as that of the testes. Thus, it may be concluded that the pineal gland of this ophidian participates in the regulation of seasonal testicular cycle, probably via the hypothalamo-hypophyseal axis, and hence the effects of pinealectomy are seasonal and a reproductive phase-dependent phenomenon.

Animals↗
PubMed1988

Lipids of dermatophytes. III. Sterol-induced changes in the lipid composition and functional properties of Epidermophyton floccosum.

Sterol supplementation, alone or in the presence of cerulenin, resulted in an increase in the total sterol content of Epidermophyton floccosum. While the total phospholipid levels of E. floccosum exhibited only marginal changes with sterol supplementation, the fatty acid profiles of these phospholipids were highly varied. In the presence or absence of cerulenin, the oleic acid content of phospholipids were increased significantly by cholesterol supplementation, whereas linoleic acid levels were enhanced by ergosterol supplementation. These variations resulted in higher unsaturated/saturated phospholipid fatty acid ratios in sterol-supplemented cells. The uptake of labeled amino acids (aspartic acid, lysine, glycine) was influenced by sterol supplementation. Alterations in the number of binding sites for the membrane probe, 1-anilino-naphthalene-8-sulfonate (ANS), were seen based on Scatchard plot calculations. The results indicate a correlation between sterol-induced changes in membrane lipid composition and function.

Amino Acids↗
PubMed1988

Acute phosphine poisoning following ingestion of aluminium phosphide.

1. Eight cases of phosphine poisoning following ingestion of aluminium phosphide tablets for suicidal attempt are described. The mean age of the patients was 23 years (range 14-25). 2. The clinical picture consisted of gastritis, altered sensorium and peripheral vascular failure in all cases, cardiac arrhythmia (3), jaundice and renal failure (1 each). Six patients died, the mean hospital stay was 19 h (range 4-72). 3. Post-mortem examination was performed in two patients, revealing pulmonary oedema, gastrointestinal mucosal congestion, petechial haemorrhages on the surface of liver and brain. Histopathological changes included pulmonary oedema, desquamation of the lining epithelium of the bronchioles; vacuolar degeneration of hepatocytes, dilatation and engorgement of hepatic central veins, sinusoids and areas showing nuclear fragmentation. 4. The clinical picture of aluminum phosphide poisoning is described and precaution in the distribution and use of this pesticide recommended.

Adolescent↗
PubMed1988

Analysis of electrophoretypes of rotavirus from diarrhoeic faeces of neonatal buffalo calves in India.

Two distinct rotavirus RNA electrophoretypes were revealed by polyacrylamide gel electrophoresis followed by silver staining (PAGE-SS). The rotavirus was sampled from the faeces of buffalo calves in India from October, 1986 to January, 1987. In the buffalo farm under study one electrophoretype was prevalent first and then the second appeared after the disappearance of the first electrophoretype. For the detection of rotavirus in the diarrhoeic faeces of buffalo calves PAGE-SS was found more sensitive when compared with agar gel immunodiffusion and discontinuous counter immunoelectrophoresis tests.

Animals↗
PubMed1988

Effect of melatonin and 5-methoxytryptamine administration on the testis and pineal gland activity of the fresh-water snake, Natrix piscator.

Effects of melatonin (aMT) and 5-methoxytryptamine (MT) were studied on the testicular activity cycle of the fresh-water snake, Natrix piscator. The subcutaneous implantation and the injections (morning as well as evening) of these two methoxyindoles prevented testicular recrudescence, retarded the testicular active phase, and accelerated the rate of regression of testes, while having no effect on the inactive testes. Contrary to this, these two compounds increased the pineal gland weight during different reproductive phases. These results revealed that both aMT and MT, whether they were continuously released through silastic capsule implants or administered through daily periodic injections, produced inhibitory effects on the testicular function.

5-Methoxytryptamine↗
PubMed1987

Reversal of cerulenin-induced inhibition of phospholipids and sterol synthesis by exogenous fatty acids/sterols in Epidermophyton floccosum.

Cerulenin, a specific inhibitor of fatty acids and sterol biosynthesis inhibited the growth of Epidermophyton floccosum, which was reversed when growth medium was supplemented with palmitic acid and sterols. Unsaturated fatty acids partially restored the growth. Cerulenin inhibited both phospholipid and sterol biosynthesis (60-70%) at the minimum inhibitory concentration (0.5 microgram/ml) as demonstrated by [32P]orthophosphoric acid and [14C]acetate incorporation into the respective lipids. Cerulenin-induced inhibition of phospholipid and sterol synthesis was dose dependent up to 0.5 microgram/ml. Exogenously supplied fatty acids and sterols restored the biosynthesis of phospholipids in cerulenin-treated cultures, while that of sterols was enhanced. The biosynthesis of both saturated and unsaturated fatty acids was inhibited by cerulenin.

Acetates↗
PubMed1987

Effect of choline, ethanolamine and serine supplementation on the membrane properties of Microsporum gypseum.

Phospholipid bases, choline and ethanolamine, when supplemented in the growth medium of Microsporum gypseum resulted in an increase in the corresponding phospholipid and total phospholipid content. However, when serine was supplemented, marginal changes were observed. The fatty acid profile of phospholipids remained almost unchanged. The uptake of lysine, leucine and aspartic acid in the spheroplasts of choline- and ethanolamine-grown cells was higher as compared to the control. 1-Anilinonaphathalene-8-sulfonate (ANS) binding to the spheroplast membrane, as calculated from Scatchard plots, demonstrated an increase in the number of binding sites in choline- and ethanolamine-grown cells, while a decrease was observed in the serine-supplemented cells. The results are discussed in terms of the effect of phospholipid polar head group composition on the membrane structure and function of this fungus.

Amino Acids↗