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Biomedical subjects

R Pacheco

Publications and source records attributed to R Pacheco.

29 records · Page 2Linked to original sources
PubMed1990

Recurrent lesions in human Leishmania braziliensis infection--reactivation or reinfection?

Strains of Leishmania braziliensis subspecies isolated from initial and recurrent lesions in 24 patients from the Pacific coast of Colombia were examined for distinguishing polymorphisms by enzyme electrophoresis, restriction endonuclease analysis of kDNA, and molecular karyotyping of nuclear DNA. Recurrent strains from 12 patients (50%) were identical to the initially infecting strain by all methods of characterisation. Phenotypic and genotypic identity, together with clinical data, support endogenous reactivation as the mechanisms of recurrent disease in these 12 patients. 5 of the 24 (22%) recurrent strains differed from the initial strain by all methods. The remaining 7 strain pairs, not separated by enzyme polymorphisms, showed differing schizodeme and/or karyotype profiles. Patients whose recurrent lesions were caused by strains different from those causing the initial lesions had a significantly longer disease-free interval than patients whose lesions were caused by identical strains. Recurrent lesions occurred further from initial lesions in the former than in the latter group. Exogenous reinfection is the most plausible explanation for recurrences due to disparate organisms. These findings have important implications for both treatment evaluation and vaccination strategies for American tegumentary leishmaniasis.

Animals↗
PubMed1990

Structure and absolute configuration of pyrophen, a novel pryrone derivative of L-phenylalanine from Aspergillus niger.

Pyrophen, a novel 4-methoxy-2-pyrone derivative of L-phenylalanine isolated from cultures of Aspergillus niger, crystallizes in the orthorhombic space group P2(1)2(1)2(1), with a = 8.918(1), b = 9.199(3), and c = 18.092(4) A. The structure was solved by direct methods and the absolute configuration assigned by the Bijvoet method. Refinement gave R = 0.045 and S = 1.78 for 1677 reflections with I greater than 3 sigma (I). Though numerous other pyrone natural products are known, including other products of A. niger, this is the first report of an amino acid-pyrone derivative.

Aspergillus niger↗
PubMed1989

Attitudes of medical personnel (doctors and nurses) toward informing the terminal patient.

The right to be informed, an indispensable condition of informed consent for a given therapeutic intervention, results in numerous practical problems in extreme situations. The purpose of this study was to analyze the relevant attitudes of medical practitioners and nursing staff. A total of 108 doctors and 102 nurses ranging in age from 22 to 63 years (mean 29, 67; SD 8, 73) was randomly selected from personnel currently in practice in Murcia (Spain) and asked to respond to a 72 item questionnaire developed by the authors. The results underline the need to approach the doctor-patient relationship from standpoint more closely attuned to the real circumstances of medical practice.

Attitude of Health Personnel↗
PubMed1988

[Quality characterization of several bolognas in Mexico. II. Evaluation of the nutritive value of their proteins].

Bologna is the most widely consumed processed meat product in the State of Sonora, Mexico. In the study herein described, the nutritive value of the protein in each brand was evaluated by means of the protein efficiency ratio (PER). Additionally, protein apparent digestibility (Cr2O3) and protein digestibility, both in vivo and in vitro, were determined, as well as apparent digestible energy in the same products. Results revealed significant differences in PER (p less than 0.05) among the commercial bologna brands with respect to ANRC casein. Nevertheless, when tested against the specific contrast, no significant PER differences were detected among the six bologna brands, and the control. Neither was there any difference detected in regard to apparent digestible energy consumption, or protein digestibility in vitro or in vivo.

Analysis of Variance↗
PubMed1988

[Quality characterization of several bolognas in Mexico. III. Sensorial evaluation by an untrained panel].

In a study on the quality evaluation of bologna, the major processed meat product of greater consumption in Sonora, Mexico, the quality of commercial brands, available in local markets, was investigated. As part of it, the sensory or organoleptic aspect of factors influencing the acceptance of bologna by consumers was included. The factors considered were: flavor, texture, appearance and color. The acceptance-preference of each product was determined using a hedonic scale and a balanced incomplete block design. The results were then statistically analyzed by the F-test for difference among treatments. Student's "t" test for population means, and multiple range for acceptance and frequency of preference. Findings revealed there were differences in flavor, texture and appearance among the bologna brands. The most relevant factors causing these differences were: saltiness, fluor flavor, stickiness, dryness, and uniformity of color. Significant differences in the color scores among the different brands were also detected, but this was not the case for overall color.

Color↗
PubMed1988

[Quality characterization of several bolognas in Mexico. I. Chemical and microbiologic evaluation].

The State of Sonora, is one of the main producers of beer, cattle and pork in México. In the work herein reported, it was determined that the total processed meat consumption in Sonora was 403.69 ton/month. The main product was bologna which for this reason, was the basis of our study. Chemical and microbiological evaluations of the commercial brands of bologna, purchased in local markets, were performed, including analysis for determinations of protein, ash, nitrites, phosphates and benzoic acid content. Total mesophyllic counts, and most probable number of coliforms, Staphylococcus aureus (positive coagulase) and Salmonella sp. were also determined. The results of the chemical analysis revealed that none of the brands complied with established norms, particularly in regard to protein, carbohydrates, phosphates and benzoic acid levels which exceeded the norms. Aerobic mesophyllic counts, and most probable number of coliforms, had wide variations even within the same brand. Only one sample contained Staphylococcus aureus, and all samples were negative for Salmonella sp.

Animals↗
PubMed1979

Interaction between theophylline and oestrogen in the rat uterus.

Theophylline alone or in the presence of 0.001, 0.01, 0.1 or 1 microgram oestradiol-17 beta/100 g body wt increase uterine RNA and protein content 6 h after administration. Uterine oedema induced by physiological doses of oestradiol-17 beta was increased further in the presence of theophylline. Theophylline decreased the number of eosinophils in the blood and concurrently decreased oestrogen-induced uterine oesinophilia at doses of 0.01, 0.1, 1, 10 or 30 micrograms oestradiol-17 beta/100 g body wt. Oestrogen binding by uterine eosinophils in vitro increased in the presence of theophylline. This effect of theophylline could explain the increase of oestrogen-induced uterine oedema in vivo.

Animals↗
PubMedDate not supplied

[Hepatocarcinoma associated to fatty liver degeneration. Report of a clinical case].

We report a case of hepatocellular carcinoma associated with fatty degeneration of the liver, which presented as and surgical acute abdomen, and was operated on at "Victorino Santaella" Hospital, being the first case observed at own Institution, and the only one reported in the national and international literature from 1971 to 1995.

Adult↗