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R P Linke

Publications and source records attributed to R P Linke.

148 records · Page 9Linked to original sources
PubMedDate not supplied

Amyloid typing using antisera to prototype fibril proteins. A brief note.

Amyloid fibril proteins from three patients with generalized amyloidosis were isolated and chemically characterized by N-terminal amino-acid sequence studies in two of them. As they belonged to three different amyloid classes they served as prototypes for the preparation of antisera specific for each class. Using these antisera in immunodiffusion, amyloid fibril proteins of 15 additional cases with generalized amyloidosis have been investigated. These could be grouped into four categories: seven belonging to the amyloid A type, three to the amyloid L, lambda type, and three to the amyloid L, kappa type; the amyloid fibril proteins of two patients could not be classified by these agents and may represent still unidentified amyloid types.

Amyloid↗
PubMedDate not supplied

Intracerebral A lambda amyloidoma diagnosed by stereotactic biopsy.

In a 70-year-old patient with an intracerebral tumor, the very rare diagnosis of an A lambda amyloidoma has been made by stereotactic biopsy using immunohistochemistry with a panel of antibodies directed against amyloid fibril proteins of various origins. The production by an apparent benign monoclonal plasma cell proliferation with secretion of paraproteins into the cerebrospinal fluid is assumed. It is the 4th case in which the amyloid protein was classified as derived from immunoglobulin lambda-light chains and, to our knowledge the first in which an A lambda amyloidoma has been diagnosed intra vitam. The amyloid is restricted to the brain and no systemic involvement has been detected. Therapeutic procedures are discussed.

Aged↗
PubMedDate not supplied

Amyloid deposits of immunohistochemically different classes in the ligamentum flavum in biopsies from patients with herniated discs or lumbar spinal stenosis.

Ligamenta flava of 100 patients (n = 107 tissue blocks) were examined for the presence of amyloid by means of polarization microscopy after staining with Congo red. Ninety-three ligamenta flava were resected due to operation of herniated discs while 14 ligamenta flava were obtained after surgical treatment of lumbar spinal stenosis. Amyloid of various amounts (score I-III) and 4 morphologically different appearances were detected in the ligamenta flava of 12 patients. In 6 patients small amyloid deposits were seen along elastic fibres (pattern 1) and/or minute, comma-like deposits in certain areas (pattern 2). In 5 other patients amyloid deposits in form of large lumps were present (pattern 3) intermingled with deposits of pattern 1 and/or 2. Only 1 patient could be detected with amyloid deposits partly associated with blood vessels (pattern 4). The amyloid-containing tissue samples (n = 13) were immunohistochemically analyzed using anti-AA-, anti-A lambda-, anti-A kappa-, anti-ATTR- and anti-A beta 2m-antibodies which are able to identify the major known amyloid classes of tissue sections. Tissues with pattern 1 and 2 amyloid deposits did not react above background with any of the antibodies. In contrast, the 5 patients with pattern 3 amyloid stained strongly with anti-ATTR but not with the other antisera. In this group, therefore, senile systemic amyloid (sporadic ATTR) was also identified which had not been detected at this site before. Consistent with this finding is the advanced age of these patients which was seen to be significantly higher than the average age of the entire group of patients examined.

Adult↗