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Biomedical subjects

R P Fule

Publications and source records attributed to R P Fule.

At least 19 recordsLinked to original sources
PubMed2002

Change in antimicrobial resistance pattern of Salmonella Typhi in central India.

BACKGROUND & OBJECTIVES: Multidrug resistant Salmonella Typhi infections have been reported worldwide in the last decade. In a study from central India in 1991 multidrug resistant S. Typhi were reported to account for 91 per cent of patients of enteric fever. The present study was planned to study the antimicrobial susceptibility pattern, phage types and biotypes of strains of S. Typhi prevalent in this region. METHODS: A total of 54 isolates of Salmonella were recovered from 1468 blood samples of patients suspected to have enteric fever and admitted in the Government Medical College and Hospital, Nagpur. Antimicrobial susceptibility pattern of these isolates was studied by disc diffusion test. Phage typing and biotyping of the first 33 strains was done at the National Salmonella Phage Typing Center, New Delhi. Minimum inhibitory concentration of chloramphenicol was determined by agar dilution method. RESULTS: Of 54 isolates of Salmonella, 51(94%) were S. Typhi and 3 (6%) were S. paratyphi A serotype. Of the 30 isolates of S. Typhi subjected to phage typing, two phage types E1 23 (77%) and A 7 (23%) were found to be prevalent in this region. Multidrug resistance was observed in 12 (22%) strains of S. Typhi. Thirty five (68%) strains were sensitive to chloramphenicol, ampicillin, gentamicin, cotrimoxazole, cefotaxime and ciprofloxacin. Resistance to two antibiotics was observed in 4 (8%) strains. Cefotaxime resistance was observed in one isolate and gentamycin resistance in two, while none of the isolates was found to be ciprofloxacin resistant. INTERPRETATION & CONCLUSION: Multidrug resistance in S. Typhi has decreased from that reported in 1991 though there is a still a small percentage of strains which continue to be multidrug resistant.

Anti-Bacterial Agents↗
PubMed1998

Study of proteases and other enzymes of Vibrio cholerae 01 E2 Tor and 0139 serotypes isolated in Yavatmal (Maharashtra).

V. cholerae 01 E1 Tor isolated during Cholera epidemic of 1994 and V cholerae 0139 serotype isolated during 1993 epidemic were subjected to the study of proteases and other enzymes. Out of 26 strains of V. cholerae 01 studied, gelatinase and caseinase activity was seen in 100 and 69.23 percent strains respectively. All strains showed catalase and oxidase activity. Of the other enzymes studied 19.23, 65.38 and 57.69 percent strains were positive for DNAse, lipase and phosphatase respectively. None of the strains showed lecithinase activity. Similar findings were observed in 22 strains of V. cholerae 0139 except all strains were positive for phosphatase activity. Role of enzymes in virulence is suggested.

Bacterial Proteins↗
PubMed1997

Changing pattern of Vibrio cholerae serotype EL TOR and 0139 in Yavatmal (Maharashtra, India) during 1992 to 1994.

During 1992, 1993 and 1994 a total of 65, 123 and 142 faecal samples respectively yielded 9, 54 and 87 strains of V. cholerae. Simultaneous occurrence of EL TOR vibrio and non 01 (i.e. 0139) was noted during 1993 and 1994 with variations in relative and absolute prevalence of each serotype. Seasonal incidence is fairly consistent in this region. Both V. cholerae serotypes 01 and 0139 showed resistance to one or more drugs.

Cholera↗
PubMed1996

Study of pathogenicity markers of staphylococci isolated from clinical specimens.

A total of 165 strains of coagulase positive staphylococci (CPS) and 39 strains of coagulase negative Staphylococci (CNS) isolated from various clinical specimens studied for biochemical and enzyme profile showed overlapping of the key characters of pathogenicity. Anaerobic mannitol fermentation (69.20%), phosphatase (58.97%) and penicillinase (58.97%) production was remarkable amongst CNS. Both CPS and CNS showed increased resistance to penicillin and other antimicrobials. Besides increased frequency of isolation of CNS from pathological specimens, they elaborate singly or in combination, the recognized virulence factors.

Coagulase↗
PubMed1995

Outbreak due to Vibrio cholerae E1 Tor & serotype O139 in Yavatmal (Maharashtra) during June-July, 1994.

A total of 65 strains of V. cholerae were isolated during June-July 1994 at Yavatmal (Maharashtra). Of the 65 strains isolated, 62 were 01 El Tor Vibrios, while three were non 01 serotype 0139. The novel epidemic strain designated as 0139 reported during the outbreak in 1993, has been supplanted by the usual El Tor Vibrio during the present outbreak while 0139 serotype has remained sporadic.

Cholera↗
PubMed1995

Study of bacteraemia using conventional and biphasic culture methods.

A total of two hundred blood samples collected from patients presenting with febrile illness were followed by culture using traditional and biphasic culture methods. Use of biphasic medium was observed to be of dual advantage as it grew pathogens earlier (28.5 percent in 18 hours, 63 percent in 36 hours of incubation) as well as in more number of cultures i.e. higher isolation rate (78 percent) when compared to traditional method.

Bacteremia↗
PubMed1991

Nosocomial infection due to salmonella senftenberg (case report).

Besides various clinical manifestations and made of spread of Salmonella infections, isolation of S. senftenberg a rare serotype from patients hospitalised for surgical procedures indicates nosocomial infection. A prolonged stay in hospital with surgical trauma facilitated the organism to cause infection in the compromised host.

Adult↗
PubMed1990

Drimarene brilliant blue--a better pre-stain in protein separation and visualization.

Prestaining of human serum proteins with a new reactive dye Drimarene Brilliant Blue (DBB), was standardized employing 940 separations and examining 30 variables. Under the critical condition, the serum and the soluble dye (0.1 g/100 ml in working Tris-glycine buffer, pH, 8.3), was mixed in equal proportion, conjugate warmed at 40 degrees C for 2 hr and a 30 microliter of the sample electrophoresed by disc electrophoresis. The method when compared with prestaining by Remazol Brilliant Blue (RBB) and postelectrophoretic staining by Amido Black (AB) in 50 normal sera, revealed that the discs stained with DBB were intense and well defined and appeared in 2 hr on a sparkingly clear gel. Quality of resolution was better than RBB and AB. Protein bands eluted from the DBB prestained gels retained their immunoreactivity. The dye-protein complex of albumin and transferrin produced high-titre monospecific antisera in rabbits.

Anthraquinones↗