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Biomedical subjects

R Moret

Publications and source records attributed to R Moret.

28 records · Page 2Linked to original sources
PubMed1979

Telolysomes in cultured iris epithelial cells and in the TVI cell-line.

Iris epithelial cells of adult newts, which are fully differntiated melanocytes and non-dividing, become dedifferentiated and converted into lens cells when put in culture. A recent study shows that this dedifferentiation is based on an autophagic process which is associated with proliferation and mainly affects melanosomes. The present report shows that in primary culture of iris epithelial cells after the majority of melanosomes have disappeared, myelinoid bodies, which are interpreted to be telolysosomes of autophagic nature, appear in high frequencies. This suggest that in these cells autophagy persists after the loss of melanosomes. A possible connection of this type of autophagy with the differentation of lens fiber which occurs in this culture is discussed. In the TVI cell line which is believed to be derived from the same cell type, but devoid of melanosomes, similar myelinoid bodies are a characteristic cell component, suggesting that the tendency for autophagy is inherited in theis cell line.

Animals↗
PubMed1978

Autophagy in dedifferentiating newt iris epithelial cells in vitro.

The ultrastructure of dedifferentiating iris epithelial cells of adult newts was studied in vitro, under the conditions which allow subsequent conversion into lens cells. The dense population of melanosomes which characterize the normal differentiated cells are progressively lost before the cells convert into lens cells. The loss of melanosomes is accompanied by the appearance of a layer of material of intermediate density surrounding the melanosomes. Subsequently vacuoles of various sizes appear in which melanosomes, ribosomes, and multivesicular bodies are sequestered. Further observations suggest that these affected organelles are destined for exocytosis. The cytochemical test for acid phosphatase is positive in the immediate vicinity of melanosomes, in the melanosome clusters, and in the multivesicular bodies. On the other hand, normal iris epithelial cells in situ are negative to the acid phosphatase test. The results are interpreted to indicate that autophagy and exocytosis of melanosomes and other organelles occur during the dedifferentiation of iris epithelial cells.

Acid Phosphatase↗
PubMed1976

An established cell line from the newt Notophthalmus viridescens.

An established cell line, derived from the dorsal iris of the eastern North American newt, Notophthalmus viridescens, is described. The cells display an epithelial-like behaviour in culture, grow relatively slowly, possess considerably larger chromosomes than mammals and are heteroploid, although some near-diploid cells are present in the culture. The line is characterized by a strong tendency to overlapping and aggregation in spite of its origin from adult tissue.

Animals↗
PubMedDate not supplied

Natural antibody response to Plasmodium falciparum Exp-1, MSP-3 and GLURP long synthetic peptides and association with protection.

A longitudinal study was undertaken in Burkina Faso among 293 children aged 6 months to 9 years in order to determine the correlation between an antibody response to several individual malarial antigens and malarial infection. It was found that the presence of a positive antibody response at the beginning of the rainy season to three long synthetic peptides corresponding to Plasmodium falciparum Exp-1 101-162, MSP-3 154-249 and GLURP 801-920 but not to CSP 274-375 correlated with a statistically significant decrease in malarial infection during the ongoing transmission season. The simultaneous presence of an antibody response to more than one antigen is indicative of a lower frequency of malarial infection. This gives scientific credibility to the notion that a successful malaria vaccine should contain multiple antigens.

Animals↗