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Biomedical subjects

R Moog

Publications and source records attributed to R Moog.

At least 55 records · Page 3Linked to original sources
PubMed1997

Leukocyte filtration of single-donor platelet units through an integrated filter system for the Fresenius AS 104 blood cell separator.

BACKGROUND AND OBJECTIVES: White cell depletion of platelet concentrates is widely applied to reduce the incidence of transfusion complications. We investigated a new plateletpheresis kit with an integrated filter for leukocyte depletion of platelets. MATERIALS AND METHODS: Parameters of product quality were investigated before and after in-line filtration of 70 single-donor platelet (SDP) units obtained by apheresis. We studied three different filter batches manufactured for the Fresenius AS 104 blood cell separator, and evaluated different softwares and interface positions with respect to platelet (PLT) yield and white blood cell (WBC) contamination. RESULTS: PLT yields before and after filtration was not statistically different in the protocols studied. WBC contamination after WBC depletion was in the range of 10(4)/SDP unit. Glucose, lactate dehydrogenase, and the morphology score were not statistically different before and after filtration. CONCLUSIONS: In-line WBC filtration of SDP units sufficiently reduced WBC below 5 x 10(6)/unit. The study allowed definition of the filter composition and apheresis parameters to optimize PLT yield and minimize WBC contamination.

Filtration↗
PubMed1996

Bronchogenic cancer in the elderly: operative risk and long-term prognosis.

Surgery for bronchogenic cancer raises the question of benefit to elderly patients. The present study reviews a cohort of 223 patients aged 70 years and more (range: 70-84), who underwent thoracotomy for pulmonary malignancy over a 10-year period. The aim of the study was to evaluate both operative risk and 5-year survival. Medical history was negative in 29% of the patients; 26% had a history of cardio-vascular disease, and 19% had a history of malignancy in complete remission. Tumor histology was squamous-cell carcinoma for 70.4%, adenocarcinoma for 24.2%, large-cell carcinoma for 3.6%, and small-cell carcinoma for 1.3% of the patients. 48.4% of patients were in stage I, 17.2% in stage II, and 30.3% in stage III. Exploratory thoracotomy was carried out in 5.8% of patients. A resection was achieved in 210 patients (pneumonectomy in 28.5%, lobectomy in 71.5%). Operative mortality was 7.2% for the whole series, 10% after pneumonectomy and 6.6% after lobectomy. Mortality was similar below and above 75 years. Overall 5-year survival was 32.9% (45.7% for stage I, 36.3% for stage II, and 13.8% for stage III). Survival was not influenced by age, symptomatic or asymptomatic presentation, medical history, and in particular not by history of malignant disease. Although operative mortality is slightly increased when compared to younger patients, long-term results legitimize surgery for bronchogenic cancer in the elderly.

Aged↗
PubMed1996

[Common mesentery: clinical and therapeutic aspects in children. Apropos of 58 cases].

Fifty-eight children with isolated malrotation of the midgut were treated in our service between 1970 and 1993. They presented with acute bilious vomiting in 75% of cases. The remaining 25% consulted for various digestive symptoms. Diagnoses were established by an upper gastrointestinal radiographic study. Prognoses were good whenever an emergency operation was performed.

Barium Sulfate↗
PubMed1996

[Small bowel obstruction and laparoscopic treatment in children].

From 1992 to 1996, 15 children with an acute small bowel obstruction were treated by laparoscopy. There were 10 boys and 5 girls between 6 to 15 years. They had been formerly operated in the neonatal period (4 cases), for appendectomy (9 cases), or ureteronephrectomy for Wilm's tumor (1 case), and caudal pancreatectomy after a traumatism (1 case). Thirteen times it was the first obstruction and twice the second or more (but never treated by surgery). Conversion in laparotomy was necessary in 4 cases. There were no operative complication. We noted a quick recovery (12 hours), an early discharge (3.5 days), and a good postoperative comfort. Laparoscopic viscerolysis for acute small bowel obstructions seems to be a good treatment in children.

Adolescent↗
PubMed1995

Evaluation of the single needle procedure in plateletpheresis with the fresenius AS 104 blood cell separator.

The aim of the present study was to evaluate the single needle (SN) procedure for plateletpheresis with the AS 104 blood cell separator. One hundred fifty-seven platelet collections with different protocols were carried out and analysed with respect to platelet yield, separation efficiency, leukocyte contamination, anticoagulant infused, and donation time. SN plateletpheresis resulted in a mean platelet yield of 2.6-3.1 x 10(11) with separation efficiencies of 49.2-57.4%. The mean leukocyte contamination ranged from 2.8 to 40.5 x 10(6). Parameters of product quality were investigated in 14 platelet concentrates (PC). Determination of glucose, lactate, lactate dehydrogenase (LDH), and morphology score showed good quality of the PC.

Adult↗
PubMed1995

In-line filtration of platelet concentrates obtained with the Omnix blood cell separator.

The quality of platelet concentrates (PC) obtained with the blood cell separator Omnix was investigated before and after in-line filtration. PC were filtered 2h (protocol A) and 4 h (protocol B) after the termination of apheresis. Platelet (PLT) yield after filtration was similar in both protocols (median 3.7 vs. 3.4 x 10(11)). Median white blood cell (WBC) contamination after leucocyte depletion was 0.07 x 10(6) (range 0.02-3.27 x 10(6)) in protocol A and 0.06 x 10(6) (range 0.02-2.1 x 10(6)) in protocol B. Glucose, lactate, lactate dehydrogenase, morphology score and pH value were not statistically different before and after filtration in both protocols. We conclude that in-line filtration results in sufficient leucocyte depletion of the PC. The prefiltration storage time did not influence the studied parameters of product quality.

Blood Cells↗
PubMed1995

Comparison of different protocols in plateletpheresis with the haemonetics MCS 3p blood cell separator with respect to parameters of product quality.

BACKGROUND: In thrombocytapheresis, intermittent flow cell separators produce platelet concentrates (PC) with a higher leucocyte contamination than continuous flow blood cell separators. The new discontinuous flow cell separator Haemonetics MCS 3p offers a low-leucocyte solution for PC. The quality of PC obtained by the MCS 3p was investigated in this study. DESIGN: Prospective study. SETTING: Haemapheresis Unit of a University Clinic. PATIENTS: Healthy blood donors from the haemapheresis unit. MATERIALS AND METHODS: Platelet (PLT) yield, separation efficiency and white blood cell (WBC) contamination were studied in three different protocols. Two protocols used a haemocalculator, which calculated the target volume based on the donor's physical characteristics and the desired PLT yield for the procedure. Protocol I used 3,000 ml as target process volume and protocol II 3.3 x 10(11) as desired PLT yield. Protocol III was used without haemocalculator. Glucose, lactate, lactate dehydrogenase (LDH), morphology score and pH value were analysed to investigate the quality of the PC. RESULTS: Platelet yield and separation efficiency were not statistically different in the three protocols. Leucocyte contamination was lowest in the protocol without haemocalculator (median: 3.15 x 10(6), range 0.4-20.8 x 10(6)). Glucose, lactate, LDH and pH were not statistically different in the three protocols. Morphology score was best in protocol III. CONCLUSIONS: PLT collection with the MCS 3p blood cell separator results in sufficient thrombocyte yields. Using the haemocalculator we were not able to achieve the desired platelet yield. For this reason, and because of the higher WBC contamination in protocol II we prefer PLT collection without the haemocalculator. The quality of the platelet concentrates was good with respect to the parameters glucose, lactate, LDH, morphology score and pH.

Adult↗
PubMed1995

[Sources of error in measuring reference values].

For the solution of many problems it is essential to separate the reaction to a stimulus from the spontaneous circadian rhythm of the variable. But disturbing masking effects evoked by the actual activity level and after effects of earlier activities (masking effects) may obstruct such separations. Several protocols have been already described to reduce masking effects. They differ mainly in the quantity of the standardized activity. Those, which try to avoid masking due to sleep, have to use higher levels of activity to keep the subjects awake. Experimental comparisons of masking effects due to activity and due to sleep as well as to sleep deficits clarify that sleep is a less potent masking agent as often expected and may be allowed in routines designed to reduce masking effects. Masking effects due to sleep deficits and moderate activities are commonly underestimated and should be avoided as far as possible in such routines. Besides the circadian changes of reagibility, the extent of masking effects (even on body temperature) questions the usage of baseline measurements. Sleep can be investigated under reduced masking effects.

Bias↗
PubMed1994

A comparison of four PTSD measures' convergent validities in Vietnam veterans.

We compared the convergent validities of four commonly used post-traumatic stress disorder (PTSD) measures in 80 help-seeking Vietnam veterans by contrasting their intercorrelations. When scored as continuous severity or frequency measures, the Mississippi Scale for Combat-related PTSD's and the Post-Traumatic Stress Disorder Interview's (PTSD-I's) concordances with other measures were similar to one anothers' and generally larger than those of either the Diagnostic Interview Schedule (DIS) PTSD module or the MMPI PTSD scale. However, when used only to identify stress disorder's presence or absence, the four techniques' concordances were nearly identical. This suggested that the four measures have similar convergent validities when used simply to identify PTSD, but that the PTSD-I and Mississippi scale offer better convergent validity than the MMPI or DIS instruments when used as severity measures.

Adult↗
PubMed1994

Results with new software for plateletpheresis systems.

New software for the cell separators A 201 and CS 3000 Plus have been developed by Baxter. Fresenius recently offered a single needle option for the AS 104 device. The results of separation are described with respect to total platelet yield, efficiency and cell contamination of the platelet concentrate (PC). Two software programs were evaluated in both the A 201 (version 5.12 and 5.13) and AS 104 cell separators (version 4560 with different separation parameters according to protocol IV and V). With software version 5.12, the A 201 collected 2.0 +/- 0.9 x 10(11) platelets in 200 mL plasma whereas 2.3 +/- 0.7 x 10(11) platelets could be harvested with version 5.13. The separation efficiencies were 32.1 +/- 11.6 and 34.7 +/- 10.7%, respectively. The leucocyte contamination was 5.6 +/- 4.6 x 10(8) with software version 5.12 and 4.9 +/- 7.6 x 10(8) with the 5.13 version. With the CS 3000 Plus cell separator, 2.9 +/- 0.8 x 10(11) platelets (efficiency 38.4 +/- 8.2%) could be harvested in 208 +/- 2 mL plasma. The white blood cell contamination was extremely low (1.4 +/- 3.2 x 10(6). There was a high separation efficacy with the Fresenius single needle procedure of 59.1 +/- 10.6% (protocol IV) and 58.7 +/- 7.3% (protocol V), respectively. The total number of platelets sampled in 338 +/- 21 mL plasma was 3.1 +/- 0.8 x 10(11) in protocol IV.(ABSTRACT TRUNCATED AT 250 WORDS)

Evaluation Studies as Topic↗
PubMed1994

[Results of cell separation with new single needle techniques in comparison with a current dual needle technique].

BACKGROUND: In plateletpheresis, new single-needle techniques have been introduced for the cell separators A 201 and AS 104. These techniques were compared with the well established CS 3000 Plus cell separator, which uses the continuous flow system. MATERIAL AND METHODS: Donation time, ACD consumption, platelet yield, separation efficiency, erythrocyte and leukocyte contamination were studied in different protocols with the dual- and single-needle procedure. RESULTS: Total amount of thrombocytes and separation efficacy were lowest with the A 201 device. The separation efficiency was best with the single-needle version of the AS 104 separator due to reprocession of the blood during the return cycle to the centrifuge. Leukocyte contamination was lowest with the standard procedure of the CS 3000 Plus device. CONCLUSIONS: The single-needle technique of the AS 104 blood cell separator resulted in sufficient platelet collection. The purity of the platelet concentrates collected with the A 201 and AS 104 machine has to be improved.

Cell Separation↗
PubMed1994

Biocompatibility studies of the single-needle technique in plateletpheresis with the Fresenius AS 104 blood cell separator.

To investigate the biocompatibility of the single-needle technique of the Fresenius AS 104 blood cell separator we analyzed coagulation, complement and hemolysis parameters prior to, during and after five thrombocytaphereses. The data were compared with results of the same parameters from identical donors in the dual-needle procedure. The analysis of bilirubin, lactate dehydrogenase, hydroxybutyrate dehydrogenase and haptoglobin showed no relevant hemolysis. However, changes of the concentrations of coagulation factors XII and XI indicated an activation of coagulation. This was confirmed by an increase of the thrombin-antithrombin III complex during apheresis. No significant change in the complement split product content of the samples obtained during apheresis was found. Complement factors, glucose, lactate, leukocytes, morphology score and thrombocytes were measured in platelet concentrates. The comparison of the platelet products showed no significant differences except for leukocyte contamination. Glucose and lactate concentrations were similar in both procedures. Levels of complement activation markers were not statistically different.

Bilirubin↗