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Biomedical subjects

R Moody

Publications and source records attributed to R Moody.

14 recordsLinked to original sources
PubMed1999

Isolation of developmental mutants of the ascidian Ciona savignyi.

Ascidians have been used extensively as model animals for experimental embryology. We report here the results of a pilot study with the aim of developing genetic methods for the ascidian Ciona savignyi. The chemical mutagen N-ethyl-N-nitrosourea (ENU) was used to induce point mutations. F1 animals, produced by using sperm from ENU-treated animals to fertilize untreated eggs, were grown to reproductive age. Sperm and eggs collected from the hermaphrodite F1 adults were used to generate self-fertilized F2 broods, which were then screened for recessive, zygotically acting mutations. Animals carrying potential mutations were outcrossed to wild type to test for the heritability of the phenotypes. We report on a number of mutants isolated using this method, including several with abnormalities in tail and notochord development.

Animals↗
PubMed1999

Mutations affecting tail and notochord development in the ascidian Ciona savignyi.

Ascidians are among the most distant chordate relatives of the vertebrates. However, ascidians share many features with vertebrates including a notochord and hollow dorsal nerve cord. A screen for N-ethyl-N-nitrosourea (ENU)-induced mutations affecting early development in the ascidian Ciona savignyi resulted in the isolation of a number of mutants including the complementing notochord mutants chongmague and chobi. In chongmague embryos the notochord fails to develop, and the notochord cells instead adopt a mesenchyme-like fate. The failure of notochord development in chongmague embryos results in a severe truncation of tail, although development of the tail muscles and caudal nerve tracts appears largely normal. Chobi embryos also have a truncation of the tail stemming from a disruption of the notochord. However, in chobi embryos the early development of the notochord appears normal and defects occur later as the notochord attempts to extend and direct elongation of the tail. We find in chobi tailbud embryos that the notochord is often bent, with cells clumped together, rather than extended as a column. These results provide new information on the function and development of the ascidian notochord. In addition, the results demonstrate how the unique features of ascidians can be used in genetic analysis of morphogenesis.

Animals↗
PubMed1994

Contact dermatitis from the old formula E45 cream.

In the past 4 years, a high incidence (118/362) of positive patch test reactions to E45 cream were noted in 2 patch testing clinics. 18/54 of those patch tested to all the ingredients demonstrated allergies to ingredients. The most frequent was triethanolamine; lanolin allergy occurring in only 1 patient. The remaining reactions may be explained as irritant reactions resulting from triethanolamine stearate (TES) formation within the cream. The irritancy of triethanolamine stearate was demonstrated in patients and controls. Conductivity studies showed that TES arises from the combination of the ingredients triethanolamine and stearic acid. The formulation of E45 cream was being changed at the time of writing, with the removal of triethanolamine from the product.

Chemistry, Pharmaceutical↗
PubMed1992

The nuts and bolts of organizing and initiating a pediatric transport team. The Sutter Memorial experience.

Specialized interfacility transport teams are capable of delivering critical care medicine to the patient at the referring hospital and while en route to the tertiary care center. To do so effectively, however, requires adequate financial and human resources; management of equipment, supplies and personnel; ongoing education for transport team members; and an aggressive quality assurance program. Team members and team management should always be prepared for worst-case scenarios, and develop a method for problem resolution as troublesome issues arise. The ultimate goal of serving the needs of the critically ill child can be consistently met only if there is a high level of commitment of all involved--from the hospital administrator and medical director to the transport coordinator and team members.

California↗
PubMed1983

Novel reciprocal translocation between chromosomes 8 and 9 found in a patient with myeloproliferative disorder.

A reciprocal translocation between the short arm of chromosome 8 and the long arm of chromosome 9 is described. What appears to be a balanced translocation with breakage and reunion at bands 8p11 and 9q34 has not to our knowledge been previously observed. The abnormality is shown to be an acquired characteristic and was found in a patient with a myeloproliferative disorder whose clinical and laboratory findings were also compatible with Ph1-negative chronic granulocytic leukemia.

Aged↗
PubMed1982

Trial of three-day and ten-day courses of amoxycillin in otitis media.

A randomised double-blind controlled trial compared three-day and 10-day courses of amoxycillin (25 mg/kg daily) in children with otitis media. Seventeen doctors from five centres admitted 84 children between the ages of 2 and 10 years. Symptoms and signs were measured on admission to the trial, on day 3, and on day 15. Mother's observations were recorded daily for 10 days. Audiograms were performed at four and 12 weeks after the end of the trial. The treatment groups showed little difference in the speed of resolution of symptoms and signs, the numbers of primary treatment failures, or the frequency of recurrent ear infections. There were no complications in either group. Most children with otitis media can probably be successfully and safely treated with no more than a three-day course of amoxycillin providing their progress is reviewed about the fifth or sixth day after treatment started. This policy could save over 1 million pounds annually in antibiotic costs.

Amoxicillin↗
PubMed1979

Intracranial hypoglossal neurilemmoma.

In a patient presenting with long tract motor and sensory signs accompanied by unilateral 12th nerve cranial palsy, we report the successful complete resection of an intra-cranial hypoglossal neurilemmoma. Our case marks the 15th reported observation of such a lesion. The value of computerized tomography in the diagnosis and localization of this type of tumor is emphasized.

Cerebral Angiography↗
PubMed1977

Ameloblastoma of the anterior maxillae.

Few generalizations may be made about the ameloblastoma. Its many varied characteristics may mimic those of many other lesions. The case presented, against all odds and expectations, proved to be highly infiltrative. The use of all available information for accurate diagnosis and sound treatment is stressed. Block resection was found to be the best treatment assuring no recurrence and minimal deformity.

Adult↗
PubMed1976

Transsphenoidal hypophysectomy: a laboratory approach and commentary.

A laboratory technique for learning and practicing the transsphenoidal approach to hypophysectomy is described. The procedure utilizes a sphenoid block with the sella turcica at its center taken from a cadaver skull. The laboratory approach stimulates the operative technique, including the use of standard instruments designed for transsphenoidal hypophysectomy. Commentary on the historical background and applications of the transsphenoidal approach to the sella turcica is presented.

Humans↗