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Biomedical subjects

R Miniero

Publications and source records attributed to R Miniero.

At least 181 records · Page 10Linked to original sources
PubMed1982

[Hepatitis B and hepatitis A markers in 33 cases of acute hepatitis B surface antigen-negative, hepatitis A virus antibody-positive viral hepatitis. Importance of immune complexes].

The authors have looked for the markers of HBV by R.I.A. method (HBsAg, anti HBsAg, HBeAg, anti HBeAg, anti HBcAg), of HAV (by measurement on two samples of HAVab or by measurement of HAVab IgM), the immune-complexes (I.C.C.) by C1q solid-phase binding assay method with E.L.I.S.A. with determination after division I.C.C. of HBsAg by R.I.A. method in 33 cases of HBsAg negative acute viral hepatitis. The 9% (3 cases) were HAV acute hepatitis, the 42,4% (14 cases) no A no B acute hepatitis, the 36,3% (12 case) were HBV acute hepatitis, in 9 anti HBcAg positive cases the I.C.C. with HBsAg positive after division resulted positive, the 12,3% (4 cases) had a positivity for HAVab by stereoconversion (2 cases) or HAVab IgM (2 cases) with HBsAg positivity after division I.C.C. This result puts a nosologic problem about the 4 cases of acute viral hepatitis, which, from epidemiological and clinical point of the view are HAV acute hepatitis.

Antibodies, Viral↗
PubMed1982

Vitamin E in beta-thalassemia.

In homozygous beta-thalassemia low serum level of alpha-tocopherol have been found. The administration of high doses of the vitamin increased the serum level, decreased lipid peroxidation and, in some case, prolonged red blood cell survival; no significant change in transfusion requirement was obtained. Only few data are available about the vitamin E in heterozygous beta-thalassemia. We have studied 131 patients aged 1 to 72 years with thalassemic trait and 218 age-matched controls. Serum level of alpha-tocopherol was statistically lower in the former. We have studied the effect of vitamin E on 10 patients. In each subject the subsequent parameters were determined before and after a three month treatment (vitamin E 400-600 mg/day) glutathione peroxidase, pyruvate kinase and creatine in erythrocytes serum vitamin E and red blood cell count. Hematological values were unchanged in all patients. In half of them biochemical parameters showed reduction of lipid peroxidation and increased erythrocyte survival.

Adolescent↗
PubMed1981

Serum immunoglobulins in homozygous beta-thalassemia.

IgG, IgA and IgM levels were studied in 187 homozygous beta-thalassemic patients and compared with age-matched normal control subjects. The not yet transfused and the polytransfused nonsplenectomized patients showed a significant increase of all Ig classes. The polytransfused splenectomized patients showed a significant increase only of IgG and IgA. The splenectomized patients, when compared to the nonsplenectomized ones, showed a significant increase of IgG, of IgA in the elder ones and a significant reduction of IgM. High ASLO and ASFLO titers were found especially in the splenectomized patients. The high Ig levels in younger not yet transfused patients, with little iron storage and normal hepatic enzyme values, demonstrate that transfusion therapy and liver damage do not play a main role in hypergammaglobulinemia. The absence of antimitochondrial, anticonnective tissue and homogeneous antinuclear antibodies seems to exclude a deficient suppressor mechanism. The hemocatheteric "overworking' of RES may reduce the antigen clearance with a consequent increase of antibody response. Splenectomy, by removing a consistent part of RES, enhances the phenomenon.

Adolescent↗
PubMed1980

Behaviour of myeloid precursors in homozygous beta thalassaemia.

Maintaining a high haemoglobin level, through a high transfusion regime, is the best method for treating thalassaemia. Not much is known about the effect of this treatment on medullary or extramedullary haemopoiesis, particularly on the extent of erythropoietic inhibition and on the behaviour of myelopoiesis. In order to analyse some aspects of the problem, we studied the myeloid stem cells (CFU-c) in the bone marrow and in the peripheral blood of children with homozygous thalassaemia, using the agar culture technique. The number of circulating CFU-c observed in 68 patients was higher than in normal subjects. This number was significantly increased after splenectomy. A positive correlation was demonstrated between the number of circulating CFU-c and the time elapsed since the last transfusion. Patients with a high Hb level displayed a marked reduction in the number of CFU-c in their peripheral blood. In 10 patients, before the beginning of transfusions, bone marrow CFU-c were lower than in normal subjects; their number increased after therapy. Most circulating CFU-c were proliferating as shown by the thymidine suicide technique.

Adolescent↗
PubMed1980

Correlation between transfusion requirement, blood volume and haemoglobin level in homozygous beta-thalassaemia.

We examined the relationship between blood volume, mean haemoglobin maintained and transfusion requirement in an extensive series (166 cases) of uniformly treated patients with homozygous beta-thalassaemia. The blood volume is increased in all the subjects and this increase appears inversely proportional to the mean haemoglobin level maintained. When this is above 12 g/dl, the blood volume is nearly normal. The transfusion requirement of patients kept between 10 and 14 g/dl is constant. Maintenance of a high haemoglobin level in thalassaemic patients inhibits erythropoiesis to a considerable degree with a reduction of the haemopoietic tissue and hence of the total blood volume.

Adolescent↗
PubMed1979

The importance of the genetic picture and globin synthesis in determining the clinical and haematological features of thalassaemia intermedia.

Twelve carriers of thalassaemia intermedia were studied. Their clinical and haematological picture was distinctly different from that in both heterozygotes and homozygotes for beta thalassaemia. Several genetic patterns were found responsible for thalassaemia intermedia: beta/delta beta thalassaemia, alpha 2 beta/beta thalassaemia-heterocellular HPFH. In a few subjects the genetic picture indicated that the patients were homozygous for beta thalassaemia, in spite of the mildness of the clinical situation. The lack of genetic uniformity was refelcted in very wide Hb A2 (2.5--8.7%) and Hb F (7.5--96.9%) ranges, as opposed to the noticeable degree of biochemical uniformity indicated by the very similar imbalance of globin chain synthesis: 0.33-0.54 for the non-alpha/alpha chain ratio in the peripheral blood. The mean for this parameter (0.43 +/- 0.05) was significantly different (P less than 0.001) from that observed in heterozygous carriers (0.60 +/- 0.10) and homozygous carriers (0.11 +/- 0.05) for beta thalassaemia. The marrow blood displayed a comparable pattern. It is therefore suggested that the severity of thalassaemia is attributable to the degree of chain synthesis imbalance.

Adolescent↗