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Biomedical subjects

R Mays

Publications and source records attributed to R Mays.

11 recordsLinked to original sources
PubMed2001

Creation of genome-wide protein expression libraries using random activation of gene expression.

Here we report the use of random activation of gene expression (RAGE) to create genome-wide protein expression libraries. RAGE libraries containing only 5 x 10(6) individual clones were found to express every gene tested, including genes that are normally silent in the parent cell line. Furthermore, endogenous genes were activated at similar frequencies and expressed at similar levels within RAGE libraries created from multiple human cell lines, demonstrating that RAGE libraries are inherently normalized. Pools of RAGE clones were used to isolate 19,547 human gene clusters, approximately 53% of which were novel when tested against public databases of expressed sequence tag (EST) and complementary DNA (cDNA). Isolation of individual clones confirmed that the activated endogenous genes can be expressed at high levels to produce biologically active proteins. The properties of RAGE libraries and RAGE expression clones are well suited for a number of biotechnological applications including gene discovery, protein characterization, drug development, and protein manufacturing.

Cell Line↗
PubMed1999

HIV immunization: acceptability and anticipated effects on sexual behavior among adolescents.

Adolescents rated hypothetical human immunodeficiency virus vaccines described as 90% and 50% efficacious and discussed how immunization might influence behavior of their peers. The low-efficacy vaccine was largely unacceptable and most believed immunization with the high-efficacy vaccine would cause increased risk behaviors. Immunization programs will need to address vaccine acceptability issues and behavioral responses to immunization.

AIDS Vaccines↗
PubMed1996

Randomized comparison of flexible versus nonflexible femoral sheaths on patient comfort after angioplasty.

Patients who undergo percutaneous transluminal coronary angioplasty (PTCA) by the femoral approach are usually required to lie flat in bed for 6 to 24 hours, which may result in significant discomfort. This study was performed to evaluate the safety and benefit of a flexible sheath that enables patients to sit at a 60-degree angle while the sheath is in place in the femoral artery. Sixty patients were randomly assigned to receive either flexible or nonflexible sheaths before PTCA. Patients with flexible sheaths were allowed to sit at an angle of 60 degrees after the procedure. Heparin management was the same in both groups. Frequency of calls to nurses for back pain was recorded for both groups. For analgesia, nalbuphine was administered in 2-mg increments. All sheaths were removed the day after the procedure. Femoral ultrasound was used to detect groin complications (hematoma, pseudoaneurysm, or arteriovenous fistula) and was performed in all patients. Baseline characteristics were similar in both groups. There were no differences in ease of sheath insertion or guide catheter movement through the sheaths. The arterial pressure waveform was not dampened in any of the flexible sheath patients while in the sitting position. Patients with flexible sheaths had fewer calls for back pain and required less nalbuphine than patients with nonflexible sheaths. Groin complications were similar in both groups. In conclusion, by allowing patients to sit up to an angle of 60 degrees, flexible sheaths have a beneficial effect in reducing back pain and the need for analgesics after PTCA.

Aged↗
PubMed1985

Prevalence of coma in black subjects.

Coma, commonly known as "being unconscious" or "out cold," is a state of consciousness characterized by a total inability to perceive incoming stimuli. A retrospective phenomenologic study found that 10 of 36 control, 19 of 36 precare, and 20 of 36 aftercare subjects (49 of 108, or 45.4 percent) had experienced coma at least once during their lives. The implications of these findings are discussed.

Black People↗
PubMed1985

Altered states of consciousness profile: an Afro-centric intrapsychic evaluation tool.

In an effort to develop an Afro-centric intrapsychic evaluation tool, the Community Mental Health Council, Inc., Altered States of Consciousness Research Team, developed a structured interview used to quantify and qualify the 17 states of consciousness(1) that occurred in black control, precare, and aftercare subjects. Differences were noted in the three groups as to the incidence, prevalence, and quality of the various states of consciousness. It was also noted that the profile obtained from the interviews yielded a sharp clinical picture of the subjects' total intrapsychic propensities.

Africa↗
PubMed1984

Prevalence of isolated sleep paralysis in black subjects.

Sleep paralysis is a state of consciousness experienced while waking from sleep or falling asleep. It is characterized by an experience of being unable to move for several seconds or minutes. This study represents the first survey to measure the incidence of this disorder in a black population of healthy subjects and psychiatric patients.

Adult↗
PubMed1978

Self-observation and self-reinforcement as sources of self-control in children.

Eight fifth- and sixth-grade black males engaged in a set of single-subject, multiple-baseline studies to determine the relative effects of self-observation and self-reinforcement. A black male college student employed the children as research assistants who would study their own behavior. The employer negotiated a series of individualized contracts with each boy. The contracts specified what self-regulation procedures the subject would perform. Overall, the children were more consistent in carrying out their contracts when they were on self-reinforcement than when they were on self-observation. Second, the children were more effective in increasing behavioral deficits than they were in decreasing excesses. Third, self-reinforcement was clearly a superior means of improving their own behavior than was self-observation.

Black or African American↗