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Biomedical subjects

R Masseyeff

Publications and source records attributed to R Masseyeff.

At least 73 records · Page 4Linked to original sources
PubMed1975

Enzymoimmunoassay of human alpha-fetoprotein.

A non-competitive method for the determination of human alpha-fetoprotein (AFP) in serum, using a pure specific antibody linked to glucose oxidase is described. When applied to human AFP, this method gives reproducible results in the range 0.7 to 15 ng/ml, in a relatively short time (6 hr). AFP sera levels of healthy human adults, pregnant women and adults with liver diseases, were tested both by enzymoimmunoassay and radioimmunoassay. In all cases, good agreement was noted between the two methods.

Adsorption↗
PubMed1975

A sandwich method of enzymoimmunoassay. I. Application to rat and human alpha-fetoprotein.

An enzymoimmunoassay (EIA) for the quantitation of soluble antigens having at least two antibody-combining sites is descirbed. This non-competitive sandwich method comprises three steps: 1) the antigen to be assayed is reacted with the antibody-coated cellulose immunosorbent, 2) the enzyme-labelled antibody is then incubated with the antigen bound to the solid phase, 3) the enzymatic activity of the immunosorbent is then measured. This activity increases with the quantity of antigen to be assayed. Examples of the application of this method to the assay of rat and human alpha-fetoprotein (AFP) are given. When applied to rat and human AFP, this assay gives reproducible results in the range of 10--1000 ng/ml and 3-1000 ng/ml respectively. AFP sera concentrations of normal and pregnant rats were assayed by EIA, radioimmunoassay RIA and rocket-immunoelectrophoresis (RIE). In all the cases good agreement was noted among these three techniques. Rheumatoid factor, whenever present, may interfere with the assay. However, the effect of this interaction can be eliminated. The advantages of the EIA method can be listed as follows: a) no pure antigen is required, b) the final color reaction is developed from the solid phase. This feature eliminates most non-specifically interfering factors, c) the range of the assay covers a 2 log-scale, d) only inexpensive equipment is used, e) results are obtained within 24 hr, f) sensitivity and reproducibility lie within a range comparable to that of RIA.

Animals↗
PubMed1975

[Bioclinical study of the electro-immunodiffusion technic for the assay of serum alpha fetoprotein].

The author discusses the result that may be expected in clinical medicine from an electro-immunodiffusion method for the detection of alpha-foetoprotein. The samples studied were obtained from pregnant women, patients with liver cell carcinoma or severe digestive diseases, whether malignant or not, and a few amniotic fluids and sera obtained from the umbilical cord. This simple, rapid method is characterized by a sensitivity threshold of about 300 ng/ml and permits one to detect about 75% of cases of liver cell carcinoma.

Adult↗