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Biomedical subjects

R M Ferguson

Publications and source records attributed to R M Ferguson.

At least 199 records · Page 11Linked to original sources
PubMed1980

Cytomegalovirus as a risk factor in renal transplantation.

A prospective study of 276 patients that were greater than 12 years old and received transplants between October 1, 1977 and September 30, 1979 has been undertaken. Any patient with clinical findings compatible with overt cytomegalovirus (CMV) disease was placed on a "CMV disease diagnostic protocol." All diagnosed cases of CMV occurring before November 15, 1979 have been analyzed. Eighty patients (29%) had overt CMV disease. Seventy-two (90%) of them contracted CMV within the first 3 months post-transplant. The incidence of overt CMV varied with donor type. Eight percent (4 of 49), 17% (8 of 48), 20% (5 of 25), 40% (46 of 115) and 43% (15 of 35) of HLA-identical (ID) siblings, non-ID siblings, child donor, cadaveric donor, and parental donor, respectively, contracted CMV disease. Overt clinical CMV disease influenced the graft function and patient survival rates significantly (P < 0.01). Several risk factors have been considered as possible indicators of CMV disease. These include age, sex, diabetic status, time of onset of CMV, donor and recipient CMV complement-fixing (CF) and indirect fluorescence (IF) titers. The same variables were analyzed to determine whether they might also predict the severity of the disease. Donor CF is the single most important risk factor. Recipient serology alone was not found to be a significant risk factor but 15 of 27 (56%) persons who had a negative titer and received a kidney from a donor with a positive CF titer contracted overt CMV. Nine of those 15 (60%) had moderate, severe, or lethal illness.

Adolescent↗
PubMed1979

Partial characterization of cytotoxic cells infiltrating sponge matrix allografts.

Adapting the Roberts and Hayry sponge allograft model, we have demonstrated the presence of an enriched, specifically cytotoxic population of cells which infiltrate rejecting sponge allografts. The number of cells infiltrating a rejecting sponge allograft peaks on day 14 after transplantation. Utilizing a short-term 51chromium cytotoxicity assay, peak antiallogeneic killing was demonstrable on day 14 also. Only T cell killing was apparent for the first 15 days after transplantation. After day 20, specific cytolysis was present which was not sensitive to anti-theta serum and complement. The infiltrating cytotoxic cells are large, specifically cytotoxic, do not proliferate in culture, do not respond to mitogen, and do not respond in mixed lymphocyte culture even to the same alloantigen to which the animal had been sensitized. In contrast, spleens from sponge-bearing animals kill poorly, respond to mitogen, and respond vigorously in mixed lymphocyte culture to specific and nonspecific alloantigens. The following hypotheses are set forth with regard to the cytotoxic lymphocytes (1) Such cells may be end stage and cannot proliferate. (2) The cytotoxic cells may kill the stimulator cells more rapidly than they can be stimulated to proliferate. (3) The sponge cell population may be enriched for nonspecific supressor cells. (4) The sponge cells may be devoid of helper T cells.

Animals↗
PubMed1979

Differential susceptibility of human peripheral blood lymphocyte subpopulations to mitogenic activation. Influence of methylprednisolone.

The inhibition of the mitogenic activation of human peripheral blood lymphocyte (PBL) subpopulations by methylprednisolone (MP) was dependent on the mitogen used. Purified human T cells were more sensitive to the effects of MP than were B cells or PBLs, especially when these cells were activated by pokeweed mitogen (PWM). MP did not function by inhibiting binding of mitogen to the cell surface. After being mitogenically activated, human lymphocytes were resistant to the effects of MP. These effects of MP were shown to be reversible. Monocytes did not provide a significant degree of protection to mitogenically activated human T cells incubated with MP. These data suggest that MP-induced inhibition of the mitogenic activation of human PBLs may be a reflection of lymphocyte heterogeneity, and that the differential sensitivity of PBLs to MP may be used to isolate functionally different subpopulations of these cells.

Classification↗
PubMed1977

Host presensitization and renal allograft success at a single institution: First transplants.

From June, 1970, to January, 1975, 399 first transplants were performed at the University of Minnesota. Of these 399, 52 had performed antibodies against HLA antigens. When the results of transplantation to these recipients were compared with the results of transplantation to a recipient group matched for age, sex, presence of diabetes, time of transplant, and donor type, no differences were observed. Similarly, no differences were observed when either group of transplant recipients were compared with all first transplant recipients during this period of time. When the results were controlled for diabetes, related or cadaver donation, or degree of presensitization, no differences were observed. Four patients developed hyperacute rejection during this period of time. All four were found to have antibodies to the donor on retrospective analysis utilizing the most sensitive antiglobulin technique. In addition, 65 donor:recipient pairs negative by standard National Institutes of Health and cross-matching techniques proved to have positive prospective cross-matches utilizing the new antiglobulin technique. The correlation between the presence of anti-HL-A antibodies and negative antidonor cross-match and early kidney loss cannot be confirmed at a single institution. The results suggest that a highly sensitive antiglobulin cross-matching technique utilizing sera drawn just prior to the scheduled transplant and cross-matching utilizing the sera bearing the greatest number of anti-HL-A antibodies will eliminate correlation between anti-HL-A antibodies and early graft rejection in the presence of a negative cross-match.

Adult↗