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Biomedical subjects

R M Fauve

Publications and source records attributed to R M Fauve.

10 recordsLinked to original sources
PubMed1992

Immunostimulatory human urinary protein.

We have previously extracted a protein from inflammatory mouse granuloma that fully protects normal mice against lethal Listeria monocytogenes infection. We now report that polyclonal antibodies directed against this protein react with a human urinary fraction that also provides full protection of normal mice against L. monocytogenes. Murine monoclonal antibodies completely inhibit the protective activity of the human urinary fraction. We have purified to apparent homogeneity a human glycoprotein of 43 kDa (HGP.43), pI = 3.2 +/- 0.2. HGP.43 injected i.v. at 250 micrograms/kg fully protected mice against a lethal inoculum of L. monocytogenes. Such resistance followed injection of HGP.43, 4 days before and, still significantly, 8 hr after Listeria infection. In scid mice lacking T and B cells, similar resistance to L. monocytogenes was observed. Inflammatory murine macrophages became cytostatic against Lewis carcinoma cells after incubation with 1.5 nM HGP-43.

Animals↗
PubMed1990

Inflammation and anti-tumor resistance. V. Production of a cytostatic factor following cooperation of elicited polymorphonuclear leukocytes and macrophages.

When Lewis tumor cells (3LL) included in a gel of polyacrylamide microbeads are injected into mice and recovered 1 day later, incorporation of 125I-UdR is strongly reduced. In contrast, no reduction is observed in irradiated mice. The cytostatic effect is non-existent in 6 hr-old granuloma (granulocytes 90%, macrophages 10%), but is maximal in 48 hr-old granuloma (granulocytes 50%, macrophages 50%). When 6 hr-old granuloma cells (which are not cytostatic) are incubated with bone-marrow-derived macrophages (BMs) (which are slightly cytostatic), considerable cytostasis against 3LL is observed, such an effect being strongly reduced if the 2 cell populations are separated. This suggests that close contact of live polymorphs with macrophages is necessary for full expression of cytostasis. No increase in cytostasis of bone-marrow macrophages is observed when BMs are incubated with a cell-free extract of freeze-thawed 6-hr-old granuloma cells. Cell contact is not necessary between granuloma cells and 3LL since the cytostatic activity is found in the supernatant of incubated phagocytic cells. Cytostasis is also observed with B16, P815, J774 cells and normal mouse bone-marrow cells. An active cytostatic fraction has been purified from the supernatant of 48-hr-old granuloma cells following molecular filtration and HPLC. The cytostatic factor has a low molecular weight (400-500 daltons), is not a peptide and presents maximum absorption at 267 nm.

Animals↗
PubMed1977

[Increased resistance in mice to Listeria monocytogenes after treatment with a fraction from an inflammatory granuloma].

The multiplication of Listeria monocytogenes in the spleen was decreased between the 3rd and the 11th day following the inflammatory reaction induced in Mice by subcutaneous implantation of talc embedded in a calcium phosphate gel into the dorsal area. A similar activity was observed after injection of SO4 (NH4)2 fractionated extracts from granuloma. The major activities were found in the 33% saturation precipitate and in the 80% saturation supernatant.

Animals↗
PubMed1976

[Immunorepulsion].

Immunorepulsion is a local immunodepression which allows embryos, tumour cells, some bacteria and many parasites to survive and grow unharmed by the host immunological surveillance system. Such a local immunodepression results from the action of immunorepellents synthesized by some aggressors. These immunorepellents are able to prevent inflammatory reactions from occurring in the vicinity of the aggressors and to impede the leucocytes from fulfilling their functions.

Bacterial Infections↗
PubMedDate not supplied

[Inflammation and host resistance against bacteria. I.--Increased resistance against Listeria monocytogenes and Salmonella typhimurium in mice, following their treatment with bradykinin, kallidin and methionyl-lysyl-bradykinin (author's transl)].

Mice pretreated with kinins are more resistant to a lethal challenge of Listeria monocytogenes. The multiplication of Listeria is decreased in the liver and spleen and the blood clearance of Salmonella typhi-murium is increased.

Animals↗
PubMedDate not supplied

[Inflammation and host resistance against tumours. II. -- Antagonism between bradykinin and a fraction isolated from the supernatant of cultured malignant cells on the spreading of macrophages (author's transl)].

When murine peritoneal macrophages are incubated in presence of 10(-6) to 10(-8) M of bradykinin, their spreading is increased. When macrophages are incubated in presence of a low molecular weight fraction of the supernatant from cultured Lewis carcinoma's cells (AP) their spreading is decreased. When macrophages are incuated first with bradykinin and later on with AP, no inhibition of spreading is observed. When macrophages are incubated first with AP and later on with bradykinin, the increase of spreading is not observed.

Bradykinin↗
PubMedDate not supplied

[Immunological responsiveness in C57BL/6 mice during the growth of a tumoural graft: the Lewis' lung carcinoma (author's transl)].

Using antibody and delayed type hypersensitivity responses to sheep red blood cells, immunological responsiveness was studied during the course of 3LL tumor growth. Acquired specific resistance to Listeria monocytogenes of tumor bearing mice was also investigated. Experimental results obtained, showed that the ability of 3LL bearing mice to establish a specific immune response is not markedly impaired.

Animals↗
PubMedDate not supplied

[Inflammation and host resistance against tumours. I. Delayed growth of Lewis tumour and inhibition of lung metastasis in mice having an inflammatory reaction distant from the inoculation site of tumour cells (author's transl)].

Following the inflammatory reaction induced in mice by subcutaneous implantation into the dorsal area of talc embeded in a calcium phosphate gel, the growth of the Lewis tumour was found to be delayed. This delay was observed even with inocula 1,000 times higher than the minimum required for the development in tumours in all animals. When 10(6) tumour cells were inoculated subcutaneously into the footpad, in contrast with normal animals, no metastases were found in the lungs if inflammatory reactions were induced 4 days before or 1 day after the inoculation of tumour cells.

Animals↗