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Biomedical subjects

R M Cunningham

Publications and source records attributed to R M Cunningham.

At least 19 recordsLinked to original sources
PubMed1989

Effects of staurosporine on protein kinase C and amylase secretion from pancreatic acini.

The effects of staurosporine, a recently isolated microbial alkaloid, on amylase secretion and protein kinase C activity of guinea pig pancreatic acini were investigated. Staurosporine at a concentration of 1 microM completely inhibited both acinar protein kinase C activity (IC50 = 5.5 +/- 1.4 nM) and amylase secretion induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) (IC50 = 4.1 +/- 0.4 nM). At this concentration, staurosporine reduced amylase secretion elicited by maximally effective concentrations of carbachol and cholecystokinin by approximately 50% but did not appreciably alter the potencies of the two secretagogues. In the presence of staurosporine, amylase secretion induced by carbachol was linear for at least 60 min. Staurosporine had no effect on amylase release elicited by the Ca2+ ionophore A23187. It did, however, inhibit secretion induced by vasoactive intestinal peptide, although with a reduced potency relative to its effects on amylase release stimulated by TPA, carbachol, and cholecystokinin (IC50 = 34 +/- 17 nM). These results indicate that staurosporine is a potent inhibitor of protein kinase C activity in pancreatic acini and that protein kinase C has an important role as an intracellular mediator of digestive enzyme secretion induced by cholecystokinin and carbachol in the acinar cell. In addition, a separate staurosporine-insensitive coupling pathway, most likely involving Ca2+, appears to be equally important and can maintain long-term secretion in the absence of functional protein kinase C activity.

Alkaloids↗
PubMed1979

It's a question of ethics.

Ethical considerations must be taken into account whenever a merger of the closing of a facility or service is contemplated/Some guiding principles that can help in making decisions.

Administrative Personnel↗
PubMed1979

This is the way it was in the 1970s.

Although regulatory, economic, and other concerns appear to have taken center stage in the health care field in the past decade, the real work of healing and comforting the sick goes on.

Cost Control↗
PubMed1979

It's not that kind of a job.

The duties of the corporation president and the hospital administrator are less publicly visible than those of the senator and the physician, but that doesn't make them less valuable.

Allied Health Personnel↗
PubMed1979

The PR war isn't the real war.

Although hospitals are losing the competition for headlines and television news time, public opinion can make itself evident with astonishing force when hospitals are threatened.

Hospital Planning↗
PubMed1979

When enough is enough.

The goals of hospitals and hospices are not necessarily incompatible, and hospice care may be an opportunity for hospitals to further humanize their services.

Aged↗
PubMed1979

Who really likes HMOs?

The growth of HMOs has been slow, mainly because of a lack of acceptance by doctors and patients. Thus, the "swing" toward HMOs is likely to continue to proceed at a stately pace.

Delivery of Health Care↗
PubMed1978

Synopsis'78--NHI: a matter of degree.

Every year around this time, health care oracles ask the same questions about national health insurance: Will we get it? What kind? How much? When? We already have NHI, the author says. The question now is whether or not we should have more.

Medicaid↗
PubMed1978

Regulation--Hey! We're in this together.

If health care professionals and legislators work cooperatively to set goals and criteria, it may be possible to resolve the confusion about regulations.

Delivery of Health Care↗