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Biomedical subjects

R M Browne

Publications and source records attributed to R M Browne.

At least 73 records · Page 4Linked to original sources
PubMed1987

An immunocytochemical study of the inflammatory cell infiltrate and epithelial expression of HLA-DR in odontogenic cysts.

The presence and distribution of lymphocytes, Langerhans cells, HLA-DR+ infiltrating cells and the expression of HLA-DR by lining epithelium was investigated in the walls of odontogenic cysts using an indirect immunoperoxidase method on acetone-fixed cryostat sections. The 23 cysts studied consisted of 14 dental (radicular) cysts, 5 keratocysts, 2 dentigerous cysts, 1 surgical ciliated cyst and 1 incisive canal cyst. The cell populations detected in the walls of all cysts were similar and consisted of HLA-DR+ macrophage-type cells and a mixture of T and B lymphocytes. Analysis of the T cell subsets revealed that in all cases the CD4+, Th/i subset predominated over the CD8+, Ts/c subset. 18/22 cyst linings contained cells expressing a Langerhans cell phenotype (CD1+). Cytoplasmic epithelial expression of HLA-DR was detected in 7/22 specimens. Neither the presence of HLA-DR+ epithelial cells nor LC were restricted to a given type of cyst. These findings indicate the occurrence of similar cellular processes irrespective of the proposed developmental or inflammatory aetiology of these lesions.

Cell Count↗
PubMed1987

A comparative study of the clinical and histological features of recurrent and non-recurrent odontogenic keratocysts.

The clinical features of 44 recurrent odontogenic keratocysts were compared with those of 228 single non-recurrent keratocysts which had been followed for 5 or more years. Histological comparisons were made with 44 non-recurrent cysts matched for age, sex and site. There were no significant differences in the age, sex and site between patients with recurrent and non-recurrent cysts. There were no significant histological differences except for a greater amount of inflammation in the non-recurrent cysts. It is suggested that operative factors have a major influence on the likelihood of recurrence.

Adolescent↗
PubMed1987

The basal cell naevus syndrome.

The basal cell naevus syndrome, classically described as a triad of multiple basal cell carcinomas, odontogenic keratocysts of the jaws and skeletal anomalies, is well known by oral and plastic surgeons and dermatologists. This paper describes the clinical features in a series of 60 patients, and highlights some problems of diagnosis and management.

Adolescent↗
PubMed1986

Pulpal response to an experimental adhesion promoter.

The pulpal response to an experimental adhesion promoter (PTP) used in conjunction with a composite resin material, Biogloss, is described, in lined and unlined cavities. Dycal was the lining and negative control material. Standardised Class V cavities were prepared in ferret canine teeth and the pulpal responses studied after 1, 2 and 4 weeks and 6 months post-operatively. Pre-treatment of cavities with PTP causes a reduction in pulpal inflammation at 7 and 14 days compared with Biogloss. There was no obvious difference at the longer time intervals. Pre-treatment with PTP delays the ingress of microorganisms at the cavity wall/material interface but does not prevent it in the long term in this situation. The effect of PTP is not altered if the fluid is allowed to dry naturally rather than by blow drying.

Acrylates↗
PubMed1985

Intra-epithelial hyaline bodies in odontogenic cysts: an immunoperoxidase study.

Fourteen dental cysts containing intra-epithelial hyaline bodies were stained for keratin, Factor VIII-related antigen, haemoglobin and fibrinogen using immunoperoxidase methods. Although the hyaline bodies were negative for all tested antigens fibrinogen was detected in the cores of some circular and polycyclic forms. These observations do not provide support for the proposed keratin, capillary endothelium or erythrocyte nature of these structures. The core staining for fibrinogen may support the theory that hyaline bodies are produced by a cellular reaction to extravasated serum.

Epithelium↗
PubMed1984

In vitro cytotoxicity of a glass ionomer cement of a new generation.

The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Testing of set samples of the material showed that its toxicity decreased rapidly with setting. ChemFil behaved in a manner similar to two older glass ionomers, ASPA and ChemBond, although it was generally slightly less irritant.

Acrylic Resins↗
PubMed1984

Autofluorescent granular cells in odontogenic cysts.

Three odontogenic cysts, two of dentigerous and one of dental type, are reported in which large numbers of cells containing autofluorescent granules were present, in both the capsule and cyst lining. Histochemical and electron microscopical studies suggest that the granules are composed of lipofuscin. Examination of 30 examples each of dental cysts, dentigerous cysts and odontogenic keratocysts indicated that the prevalence of occasional cells of similar appearance was about 40%, 50% and 17% respectively. The pathogenesis of these cells is discussed.

Adult↗