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Biomedical subjects

R M Adams

Publications and source records attributed to R M Adams.

108 records · Page 6Linked to original sources
PubMedDate not supplied

Effective cryopreservation and long-term storage of primary human hepatocytes with recovery of viability, differentiation, and replicative potential.

Despite reports of successful cryopreservation of primary human hepatocytes, existing methods do not produce sufficient recovery of viable cells to meet the needs of basic research or clinical trials of hepatocellular transplantation. We now describe a protocol for efficient cryopreservation of primary human hepatocytes using University of Wisconsin (UW) solution, fetal bovine serum, and dimethyl sulfoxide (DMSO). This method provides > 90% viability of differentiated, primary human hepatocytes 8 mo after cryopreservation as measured by trypan blue exclusion, preserves hepatocyte morphology, liver-specific gene expression (alpha 1 antitrypsin), and replication. The effectiveness of UW solution as a cryopreservative agent suggests that metabolic as well as ultrastructural factors may be important in the effective cryopreservation of primary human hepatocytes. The present method represents an effective protocol for cryopreserving differentiated primary human hepatocytes for research. This method may allow characterization and banking of human hepatocytes for clinical applications, including hepatocellular transplantation and hepatic assist devices.

Cell Differentiation↗
PubMedDate not supplied

Patch testing for occupational allergens and the evaluation of patients with occupational contact dermatitis.

With the expanding interest in the environmental causes of disease, carefully performed, differential patch testing in suspected cases of contact allergy becomes a vitally important part of the investigation. Although all of the necessary allergens have not yet been released by the FDA for U.S. manufacture and distribution, it is hoped this will occur within the next few months. The thousands of patients with work-related allergic contact dermatitis can then hope for to learn the exact source of their disease, which can then lead to suitable work adjustments.

Allergens↗
PubMedDate not supplied

Dermatitis in the microelectronics industry.

Many of the chemicals used in the semiconductor industry are strong skin irritants, and several are capable of readily inducing contact allergic sensitization. In plants in which state-of-the-art equipment is used, the hazards to production workers are minimal today, but those workers who clean, repair, and maintain equipment remain at special risk. The major potential cutaneous hazards of each step in the production of microchips are the subject of this article.

Allergens↗