Search PubMed⌕ Search

Biomedical subjects

R Lira

Publications and source records attributed to R Lira.

22 records · Page 2Linked to original sources
PubMed1986

120 and 80 kd detergent-insoluble glycoproteins as markers of differentiation and adhesion in B16 melanoma.

Surface glycoproteins correlating with cell adhesion and differentiation have now been studied in pigmented and amelanotic B16 melanoma cell monolayers and aggregates, labelled with 3H-glucosamine. Solubilization with the non-ionic detergent octyl glucoside revealed mostly adhesion-related changes, which involved a decrease in 140 and 110 kd glycoproteins in both pigmented and amelanotic cell aggregates. Nevertheless, differentiation-regulated changes were preferentially evident in the remaining detergent-insoluble glycoproteins. Pigmented cell monolayers showed a preferential increase in glycoprotein species of about 120 kd and 80 kd, which were not detected in amelanotic monolayers or in pigmented or amelanotic cell aggregates. Our findings suggest the relevance of these cytoskeleton associated glycoproteins as markers of differentiation and adhesion in melanoma cells.

Cell Adhesion↗
PubMed1984

Relationship of a novel extracellular matrix glycoprotein to cell detachment in highly metastatic B16 melanoma: modulating effect of bromodeoxyuridine.

Growth of highly invasive B16 melanoma BL6 cells with bromodeoxyuridine (BUdR) decreases in vitro cell detachment and modulates extrapulmonary growth in vivo. We now show: (1) The presence of an 80 kd glycoprotein in the Triton-insoluble matrix of control BL6 cells but not in the corresponding fractions from BUdR-treated BL6 cells and poorly metastatic F1r cells. (2) The matrix fractions from the two last mentioned cells reveal Triton-insoluble glycoproteins of about 55-58 kd. (3) Mild trypsin treatment of intact cells before matrix preparation leads to the preferential disappearance of the 80 kd component from control BL6 matrix, suggesting its extracellular localization. (4) Prevention of Triton-mediated BL6 matrix detachment by zinc chloride pretreatment, and analysis of different BL6 clones with significant metastatic behavior, also revealed the presence of 80-90 kd matrix-associated glycoproteins in control but not in corresponding BUdR-grown cultures. Since BUdR decreases cell detachment, extrapulmonary metastasis and the levels of the 80-90 kd Triton-insoluble glycoprotein species in metastatic B16 melanoma, and this matrix component is also decreased in poorly metastatic F1r cells, we propose an involvement of this glycoconjugate in tumor cell detachment and metastatic behavior.

Animals↗
PubMedDate not supplied

The sterol composition of Trypanosoma cruzi changes after growth in different culture media and results in different sensitivity to digitonin-permeabilization.

Respiration, oxidative phosphorylation. and the corresponding changes in membrane potential (deltapsi) of Trypanosoma cruzi epimastigotes grown either in liver infusion-tryptose (LIT) or brain heart infusion (BHI) culture medium were assayed in situ using digitonin to render their plasma membrane permeable to succinate, ADP, safranine O, and other small molecules. When the cells were permeabilized with 64 microM digitonin, a concentration previously used with epimastigotes, the ability of the cells grown in LIT medium to sustain oxidative phosphorylation was demonstrated by the detection of an oligomycin-sensitive decrease in mitochondrial membrane potential induced by ADP. In contrast, the cells grown in BHI medium were not able to sustain a stable membrane potential and did not respond to ADP addition. Analyses of oxygen consumption by these permeabilized cells indicated that the rate of basal respiration, which was similar in both cell types, was significantly decreased by 64 microM digitonin. Addition of ADP to the permeabilized cells grown in LIT medium promoted an oligomycin-sensitive transition from resting to phosphorylating respiration in contrast to the cells grown in BHI medium, whose respiration decreased steadily and did not respond either to ADP or CCCP. Titration of the cells grown in BHI medium with different digitonin concentrations indicated that their mitochondria have higher sensitivity to digitonin than those grown in LIT medium. Analysis of the sterol composition of epimastigotes grown in the two different media showed a higher percentage of cholesterol in total and mitochondrial extracts of epimastigotes grown in BHI medium as compared to those grown in LIT medium, suggesting the involvement of this sterol in their increased sensitivity to digitonin-permeabilization.

Animals↗