Search PubMed⌕ Search

Biomedical subjects

R Lipińska

Publications and source records attributed to R Lipińska.

At least 19 recordsLinked to original sources
PubMed1994

[Evaluation of selected immunologic parameters in healthy persons infected with mycobacterium tuberculosis bacillus].

Selected immunological parameters of peripheral blood leukocytes in 30 tb contracts and control group consisting of 30 healthy blood donors in similar age were examined. All contacts showed the exudate type Mantoux reaction (over 20 mm in diameter) and all revealed normal chest X-ray. No differences between both groups in total T cells, CD4, CD8 counts, CD4/CD8 ratio and in proliferative response to mitogens (PHA and Con A) were found. Tb contacts had the similar serum IgM and elevated IgG and IgA concentrations as compared with the controls. Tb contacts showed depressed granulocyte metabolic activity as measured by CL response to chemostatic peptide N-FORMYL-MET-LEU-PHE (FMLP) in comparison with control subjects.

Adult↗
PubMed1992

[Assessment of the value of stimulation from a preparation of Tołpa peat for treatment of trauma related osteomyelitis].

Tołpa peat preparation at the dose of 5 mg per day was administered to 10 patients with chronic posttraumatic osteomyelitis for 6 weeks period of time. Good clinical result (ceasing of the inflammatory process) was found in 4 patients but 3 of them were concurrently treated surgically. Immunological examination (angiogenesis test and chemiluminescence test) performed before the onset and after completion of the treatment with Tołpa preparation did not reveal meaningful changes indicating immunomodulating action of the preparation on the course of posttraumatic osteomyelitis.

Adolescent↗
PubMed1991

[Cellular analysis of bronchoalveolar lavage fluid. I. Detection of macrophage populations].

The aim of the study was to compare the results of routine counts of percentage of BAL macrophages, using morphological differential staining method--Pappenheim stain (M) and staining for nonspecific esterase activity (E) with those obtained by APAAP technique, using specific for monocytes (macrophages monoclonal antibodies (Ki-M1) and the complex APAAP (alkaline phosphatase--mouse anti alkaline phosphatase complex). The mean counts were comparable, although different criteria of estimation were used. Significant differences were seen in smokers when the criteria were based on morphology and expression of surface antigens. The results indicate that correct estimation of macrophage counts and their characteristics (enzymatic activity, expression of surface antigens) can only be made using many methods based on various criteria of estimation.

Adult↗
PubMed1981

The application of immunodiffusion test to the estimation of hydrolysis of mycobacteria proteins by proteolytic enzymes of mice peritoneal cells.

The possibility of applying the immunodiffusion (ID) test in agarose gel to detect the degradation of the somatic proteins of mycobacteria BCG-Poland by proteolytic enzymes of non activated and activated mice peritoneal cells was investigated. Degradation of those proteins was determined by disappearance of some precipitin lines resulting from the hydrolysis. The immunodiffusion test was found to be useful to show the degradation of protein of mycobacteria by cathepsins of mice peritoneal cells. Disappearance of 4 precipitin lines in ID test occurred after 3 h incubation of mycobacteria proteins with extracts of non activated mice peritoneal cells at pH 3, which gives evidence of degradation of some mycobacteria antigens. The extention of the time of incubation up to 24 h gave the same results. At the incubation at pH 4.5 no disappearance of precipitin lines was observed. It was found that cathepsins no specifically activated mice peritoneal cells degradate mycobacteria proteins at pH 3 and pH 4.5. Degradation occurred more rapidly at pH 3 achieving maximal effect after 6 h. The degree of degradation at pH 4.5 increased during the whole period of examination, i.e. up to 24 h of incubation time after which a disappearance of 5 precipitin lines was found. No degradation was found after incubation at pH 6 and pH 8.

Acid Phosphatase↗
PubMed1978

An attempt at the use of immunological methods for the detection of enzymes of BCG substrains.

Immunological methods have been used to visualize peroxidase and dehydrogenase activities of substrains of BCG mycobacteria. Immunodiffusion (ID) and immunoelectrophoresis (IE) in agar gel and two-dimensional immunoelectrophoresis (2D-IE) in agarose gel were employed. In all these tests part of the ID, IE, and 2D-IE preparations were stained with Amido Black 10B (AB 10B) to visualize the total number of precipitin lines and part were stained at the same time with histochemical methods for vizualization of peroxidase and dehydrogenase activities.

BCG Vaccine↗
PubMed1978

The use of two-dimensional immunoelectrophoresis for the characterization of antigens of BCG substrains.

The usefulness of the method of two-dimensional immunoelectrophoresis (2D-IEP) in comparative studies and characterization of the antigenic spectra of various BCG substrains have been estimated. The BCG substrains used were: substrain BCG-Rio de Janeiro, substrain BCG-Poland, form-rough (R) and substrain BCG-Poland, form-smooth (S). The 2D-IEP technique was found to give characteristic immunoelectrophoretic patterns of antigenic composition of the different BCG substrains. The number of precipitin peaks was usually over 20, that is more than detected by conventional immunoprecipitation techniques such as immunodiffusion and immunoelectrophoresis in agar gel (ID, IE).

Antigens, Bacterial↗
PubMed1978

Studies on the morphology of colonies of bacilli of BCG-Poland substrain. I. The influence of iron on the type of BCG-Poland colonies.

The influence of iron concentration in Sauton's medium solidified with agar on the type of colonies of BCG-Poland substrains, BCG-Rio de Janeiro, BCG-France, BCG-Denmark and BCG-Japan substrains has been examined. Of all the studied BCG substrains only the BCG-Poland substrain formed rough (R) and smooth (S) colonies. In the investigated substrains rough colonies became smaller with the decrease of iron concentration but they retained their characteristic surface roughness. The smooth colonies which in the same conditions appeared only in BCG-Poland substrain did not display such dependency on iron concentration. When the incubation period was prolonged secondary rough colonies appeared among the smooth ones, regardless of the iron concentration in the medium.

BCG Vaccine↗
PubMed1978

Studies on the morphology of colonies of bacilli of BCG-Poland substrain. II. Dissociation of BCG-Poland substrain on Löwenstein-Jensen egg medium.

The dissociation of colonies of the substrain BCG-Poland on Löwenstein-Jensen medium has been studied. It had been stated previously (Lipińska and Rzucidło, 1974) that BCG-Poland substrain undergoes a process of dissociation which manifests itself in the appearance of smooth colonies alongside typical rough colonies. Attempts have been made to obtain pure culture of both colony types. Pure cultures composed of smooth or rough colonies respectively were obtained. The morphology and diameter of the smooth colonies depended on the size of the seed inoculum spread on the medium.

BCG Vaccine↗