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Biomedical subjects

R Lewis

Publications and source records attributed to R Lewis.

At least 307 records · Page 17Linked to original sources
PubMed1979

Cervical cancer cell lines containing herpesvirus markers.

Five epithelioid cell lines were established from invasive cervical carcinoma tissue and were examined for herpes simplex virus (HSV) antigens or virus-induced changes. Immunofluorescence studies with antiserum to an HSV type 2 nonstructural polypeptide detected perinuclear antigens. In these cell lines, concentrations of a membrane fucopeptide that is regularly present in normal cells were reduced to levels similar to those previously observed in virus-transformed cells. One of the cell lines inoculated into athymic mice produced tumors.

Animals↗
PubMed1978

Genetic toxicology of bleomycin.

Bleomycin (BLM), an antibiotic obtained from Streptomyces verticillus, is of significance as an antineoplastic agent. The compound is actually the mixture of some 200 related forms which differ from each other in the amine moiety. The drug, at low concentrations, can cause elimination of bases, particularly thymine. This causes strand breakage of DNA and inhibition of cell growth. The influence of BLM on cell growth may be unrelated to the effects on DNA. In general, mitotically dividing cells show more DNA damage than non-dividing cells. G2 seems to be the most sensitive phase indicating that cell death may not be related to a direct effect of BLM on DNA replication. The antibiotic shows specific effects on chromatin and causes chromosomal damage in all sub-phases of interphase. It can affect early prophase chromosomes also. Suggestion has been made that BLM-induced breakage and cell death are similar to those induced by densely ionizing radiations. Whereas the antibiotic affects the frequency of somatic crossing over and produces micronuclei, the data on mutation induction and production of sister-chromatid exchanges do not permit classifying BLM as a potent inducer of these phenomena. The genetic effects of BLM can be modified quantitatively by thiol compounds, caffeine, hyperthermia and H2O2. It is concluded that the available data do not permit assessment of genetic damage in the offsprings of BLM-treated patients. Such studies are urgently needed, as are the studies to find out the effects of BLM on meiotic phenomena.

Animals↗
PubMed1978

Association of cytomegalovirus (CMV) infection with cervical cancer: isolation of CMV from cell cultures derived from cervical biopsy.

Cytomegalovirus (CMV) was isolated from cell cultures derived from 2 of 10 cervical cancer biopsies from patients in an advanced stage of the disease. Five serial passages were necessary before extensive cytopathic changes characteristic of CMV infection appeared. All patients tested had complement-fixing antibodies to both isolates in higher titers than to the prototype AD169 strain. 6 of 8 patients tested also had herpesvirus type 2 antibodies.

Adult↗