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Biomedical subjects

R Lallier

Publications and source records attributed to R Lallier.

At least 55 records · Page 3Linked to original sources
PubMed1976

Escherichia coli strains isolated from diarrheic piglets in the Province of Quebec.

During 1972 to 1974, 112 Escherichia coli strains isolated from diarrheic piglets were recieved from different parts of the Province of Quebec, Canada. Fifty-six strains elicited a positive gut loop response in three week old piglets and were then considered as Moon's class 1 enteropathogens, while four of the 56 remaining strains reacted only in ten day old piglets and were classified as class 2 enteropathogens. Forty-eight strains produced both a heat-labile and a heat-stable enterotoxin, while 12 isolates which included the four class 2 enteropathogens produced only a heat-stable enterotoxin. Fifty-one enterotoxigenic strains could be serogrouped using OK antisera against E. coli strains commonly associated with colibacillosis in piglets. The most common serogroups encountered were O157: K "V17"; 88a,c, O149:K91; 88a.c. O157:K"V1c, O149:K91; 88a.c, O157:K"V17"; 88 a,c or a,b and O45:K"E5"; 88a,c. No significant difference was observed in the fermentation patterns, antibiotic susceptibility, colicin production, production of a filterable hemolysin and transferable tetracycline resistance between the enterotoxigenic and the nonenterotoxigenic strains.

Animals↗
PubMed1976

Pathological and microbiological observations made on spontaneous cases of acute neonatal calf diarrhea.

The purpose of this report is to describe clinical signs, gross and microscopic lesions, bacteriological and immunofluorescence observations made on spontaneous cases of acute neonatal calf diarrhea (NCD) in dairy and beef herds. The following diagnostic tools were used: 1) direct smears of intestinal content, 2) Escherichia coli counts, 3) aerobic bacterial cultures of the small intestine and other organs (The O serogroup and the enterotoxigenicity of the E. coli isolated was determined), 4) detection of the two Nebraska NCD viruses (reo-like and corona-like) by the fluorescent antibody technique and 5) histological examination on different segments of the digestive tract. The following etiological diagnoses were suggested after post mortem examination of 55 cases of NDC (34 were submitted alive): reo-like virus only (1), reo-like virus + E. coli (4), reo-like virus + cryptosporidium (2), reo- + corona-like viruses (5), reo- + corona-like viruses + cryptosporidium (3), reo- + corona-like viruses + infectious bovine rhinotracheitis virus (1), coronavirus-like agent only (2), coronavirus-like agent + mycotic abomasitis (1), coronavirus-like agent + crytosporidium (1), E. coli only (6), cryptosporidium only (5), mycotic abomasitis (3), mycotic rumenitis + reticulitis (1) and undetermined (20). Most of the calves in the last group were submitted dead.

Acute Disease↗
PubMed1975

Relationships of intestinal enzymes and serum antitoxin to the pig response to Escherichia coli enterotoxin.

The intestinal loop technique was used to evaluate the response of three week old piglets to the heat labile (LT) and the heat stable (ST) enterotoxins produced by Escherichia coli F11(P155). The serum anti-LT activity and the lipase, amylase and trypsin activities in the jejunal lumen of these pigs were determined. Piglets responded independently ti each toxin and no relationship between these responses and serum anti-LT activity or the enzyme activities of the jejunal content could be demonstrated.

Animals↗
PubMed1973

Growth of normal and transformed rat embryo fibroblasts: effects of glycolipids from Salmonella minnesota R mutants.

Addition of glycolipids obtained from Salmonella minnesota R mutants to normal, spontaneously transformed, and SV40-transformed rat embryo fibroblasts in culture results in an inhibition of growth of transformed cells but not of normal cells. In the presence of the glycolipid with the smallest carbohydrate chain length, spontaneously transformed cells stop growing when they reach confluency. Inhibition of growth of transformed cells is inversely related to the chain length of the core sugars. Glycolipid mR595 is shown to bind with the cell membrane of transformed cells and elicits an augmentation in the intracellular level of cyclic AMP. Normal cells bind relatively less glycolipid mR595 and show a lower percent of increase in cyclic AMP due to glycolipid mR595 than do transformed cells.

Animals↗
PubMed1973

Ganglioside patterns and phenotypic characteristics in a normal variant and a transformed back variant of a simian virus 40-induced hamster tumor cell line.

Ganglioside patterns of a cloned Simian virus 40- (SV40) induced hamster tumor cell (Cl(2)TSV(5)-S), its normal variant (Cl(2)TSV(5)-R) which are Cl(2)TSV(5)-S gradually adapted to grow in the presence of 2 microg/ml actinomycin D and exhibit certain normal phenotypic characteristics, and its back variant (Cl(2)TSV(5)-RR), which are Cl(2)TSV(5)-R cells grown in the absence of actinomycin D for more than 60 passages and which exhibit greater phenotypic similarity to Cl(2)TSV(5)-S cells, have been analyzed. All three cell lines contain N(acetylneuraminyl) galactosylglycosyl ceramide (hematoside, GM(3)), N-acetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosyl ceramide (GM(2)), and a higher ganglioside tentatively identified as disialohematoside. However, Cl(2)TSV(5)-R have more GM(2) than Cl(2)TSV(5)-S whereas Cl(2)TSV(5)-RR contain an intermediate amount of GM(2). The amount of GM(2) is correlative with the activity of UDP-N-acetylgalactosamine: hematoside N-acetylgalactosaminyl transferase in the extract of the three cell lines and with their agglutination by wheat germ agglutinin.

Animals↗