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Biomedical subjects

R L Teplitz

Publications and source records attributed to R L Teplitz.

At least 109 records · Page 6Linked to original sources
PubMed1965

Sex chromatin of cone cells of human retina.

Each retinal cone cell of the female contains a sex-chromatin body not present in coinparable material from the male. Therefore the lack of detectable "patch" formation in female heterozygotes for red color-blindness (as expected from the X-inactivation hypothesis) cannot be attributed to failure of Barr-body formation.

Humans↗
PubMedDate not supplied

Metastatic behaviour of canine lung carcinoma in autochthonous and xenotransplant hosts.

Specimens from 24 chemically induced canine non-small cell lung cancers (NSCLC) were xenotransplanted into nude mice. Twenty-one tumour lines were established in serial transplantation; four were from NSCLC that arose from orthotopically induced NSCLC in four dogs, and 17 were from NSCLC that arose heterotopically in 15 subcutaneous bronchial autografts (SBA) in seven dogs. Distant metastases developed in recipients of two orthotopic NSCLC after three and eight consecutive tumour growth cycles; no metastases have occurred after three and six growth cycles of two other orthotopic tumour lines. Recipients of eight heterotopic tumour lines developed metastases after 3-9 consecutive tumour growth cycles, while no metastases have occurred after 4-11 growth cycles in recipients of nine other heterotopic tumour lines. In three instances, both metastasizing and non-metastasizing tumour lines resulted from NSCLC that arose in different SBAs in the same dog. These findings indicate that, in the canine SBA bronchogenic cancer model as expanded by tumour xenotransplantation, those molecular events involved in both the initiation and the full progression of a single cancer may be investigated serially and concomitantly.

Animals↗
PubMedDate not supplied

Gradient separation of normal and malignant cells. II. Application to in vivo tumor diagnosis.

Data were obtained concerning the usefulness of discontinuous Ficoll and Isopaque-Ficoll gradients in separating malignant from non-malignant cells from a variety of sources. Most of the material was from effusions, but there is no theoretical restriction of the methods to effusions. Animal and human material were studied, utilizing non-malignant as well as malignant material, the former acting as controls. In the discontinuous Ficoll gradient, concentration of malignant cells at a specific interface was mainly a function of cell density. Thus, while most malignant cells banded in the last two fractions, adenocarcinomas, especially those high in mucus (which decreases total cell density) banded higher in the gradient. The Isopaque-Ficoll technique also proved useful in concentrating malignant cells. The advantage of the discontinuous Ficoll gradient was its ability to separate cells from small specimens (1-10 X 10(6) cells). Contrarily, the Isopaque-Ficoll gradient was preferable for processing specimens of larger volumes.

Adenocarcinoma↗
PubMedDate not supplied

Rapid staining techniques employed in fine needle aspirations of the lung.

Fifty-two consecutive patients ranging in age from 22 to 90 years underwent percutaneous transthoracic fine needle aspiration biopsy under fluoroscopic guidance. A quick staining and rapid interpretation technique was used on samples from the 37 most recent cases. Rapid staining was accomplished within five minutes, and interpretation was available less than 15 minutes after obtaining the specimen. Successful diagnosis was obtained in 36 of 37 patients (97%). Standard methods utilizing a single aspiration sample yielded a successful diagnosis in 13 of 15 patients (86%). The improved rate of successful diagnosis following the rapid stain technique reflects the advantage of being able to determine the adequacy of specimens before releasing patients from the procedure room.

Adult↗