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Biomedical subjects

R L Teplitz

Publications and source records attributed to R L Teplitz.

At least 19 recordsLinked to original sources
PubMed1992

Prenatal diagnosis by enzymatic amplification and restriction endonuclease digestion for detection of haemoglobins A, S and C.

The detection of the single base pair mutations at codon 6 of the beta-globin gene is important for the prenatal diagnosis of sickle-cell anaemia and SC disease. A novel procedure has been designed to create a restriction site at both the beta A and beta C alleles to facilitate the discrimination of haemoglobins A, S and C. The general principle of this procedure is to enzymatically amplify genomic DNA using a modified primer containing an altered 3'-terminal nucleotide to create these restriction sites. After this modified primer has been efficiently incorporated into amplified DNA, the PCR products are digested with the restriction enzymes Ava I and Sty I. Ava I recognizes a site in amplified DNA containing a beta A allele, and Sty I recognizes a site in DNA containing a beta C allele. Since the beta A and beta C alleles can be distinguished directly by the presence of a restriction site, the beta S allele can be identified indirectly. All three beta-globin alleles are easily distinguished by size and pattern of electrophoresed fragments on agarose gels. This procedure is specific and sensitive, thus permitting rapid, economical diagnosis of sickle-cell anaemia and SC disease.

Anemia, Sickle Cell↗
PubMed1979

Genetics.

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DNA Repair↗
PubMed1979

An enzyme-linked immunosorbent assay for antibodies to native and denatured DNA.

A modification of the enzyme-linked immunosorbent assay (ELISA) is described, that permits determination of antibodies to native DNA (nDNA). The same approach can be used to measure antibodies to denatured DNA (dDNA). Poor binding of nDNA to the polystyrene solid phase has presented difficulties in using the ELISA method for assaying anti-nDNA activity (Engvall, 1976), but we find that precoating of the solid phase with protamine sulfate circumvents this problem. Assays for anti-dDNA are also enhanced by the use of protamine sulfate coated tubes. We have used the ELISA method to assay 15 SLE and 27 non-SLE sera for anti-nDNA and anti-dDNA activity. The results are compared with those obtained using the GF/A glass fiber filter assay, previously described by Lewis et al. (1973).

Animals↗
PubMed1979

Autosensitization to DNA: evidence for an immunologic basis.

A 59-year-old female with spontaneous painful ecchymoses developed ecchymoses after intracutaneous injection of washed autologous whole blood cells and calf thymus DNA. Immunofluorescent studies of the spontaneous lesions revealed granular deposits of IgM, C3, factor B and properdin at the dermal-epidermal junction but no deposits in her normal skin. T cells were decreased in number but responded normally to polyclonal mitogens and did not transform in response to DNA containing antigens. Repair of UV-damaged DNA by her lymphocytes appeared to be depressed. The findings presented here are the first immunologic abnormalities uncovered in this disorder and may help in understanding the pathogenesis of the inflammatory lesions seen in autosensitization to DNA.

Autoantibodies↗
PubMed1978

Genetics.

Explore the source record for details and available documents.

Animals↗
PubMed1978

Ataxia telangiectasia.

Ataxia telangiectasia has been described as a single-gene autosomal recessive disorder. It affects multiple systems. Several attempts to present an etiological hypothesis that will account for the multisystem involvement have been made. Those reviewed haer are (1) aberration of inductive signaling, which is predicted on a deficient mesoderm, leading to vascular and thus multiorgan effects (a critical timing of events is a basic assumption); (2) developmental imbalance, based on multisystem supression of embryological development; (3) the autoimmune hypothesis, which requires the presence of a cell surface antigen on thymic and nerve cells analogous to the thy 1.1 antigen of mouse and rat. The development of cytotoxic autoantibody to this antigen is postulated as an explanation of the pathogenesis.

Ataxia Telangiectasia↗
PubMed1978

Establishment and characterization of a cell line from the American opossum (Didelphys virginiana).

A permanent tissue-cultured cell line (designated OK) has been established from kidney tissue of an adult American opossum. The OK line has been characterized with respect to morphology, chromosome constitution, tissue-culture requirements, and attainable mitotic arrest. The cells are epithelial-like with a stable nondiploid chromosomal modal number of 23. Cells grown in Eagle's minimal essential medium with 10% fetal bovine serum have a mean doubling time of 18 hr. The cell line OK is potentially useful for the isolation and purification of the mammalian X chromosome because of the size differential between the smaller X's and the larger autosomes.

Animals↗
PubMed1978

Solubilization and developmental distribution of embryonic antigen of the chick red blood cell plasma membrane.

An antigen(s) on the surface of embryonic and newly hatched chick red blood cells was studied with antiserum absorbed with adult red blood cells. Because of the reappearance of the antigen or some cross-reactive antigen during myeloblastosis, attempts at solubilization and characterization of the antigen were pursued. Antigen was solubilized from whole red blood cell lysates or from red blood cell ghosts with 0.01 M Tris:0.1% Nonidet P40, pH 8.0. Antigen was assayed with an enhancement of agglutination assay. Enhancement apparently occurs because of available specific receptors for the antigen on newly hatched chick red blood cells. Antigen was also found to be present in plasma, and both the membrane-derived antigen and the plasma antigen were excluded from Sephadex G-100. Isoelectric focusing of the antigen extract indicated the presence of more than one molecular species with antigenic activity.

Age Factors↗
PubMed1978

NZB/WF1 hybrid autoimmune disease: a genetic analysis.

This study analyzes the role of genetic and/or viral factors as etiologic agents in systemic lupus erythematosus in NZB/NZW F1 hybrid mouse, first by a backcross within the NZ strains. A separate study involved an outcross, production of an F2 generation, and then backcross to both NZ parents. The results were assayed by histologic evaluation of renal disease. Assay techniques for anti-DNA and proteinuria showed no evidence for segregation and were not useful. Histology of the kidney showed distinct segregation patterns consistent with the interpretation of the etiology as co-dominant genetic factors. While the virus[es] was not considered etiologic, mechanisms for relating activity to the pathogenesis were discussed.

Animals↗
PubMed1976

Ultrastructural differentiation during Drosophila neurogenesis in vitro.

Drosophila melanogaster field neuroblasts differentiate in vitro, and each gives rise to a cluster of about 18 daughter neurons. Electron microscopic observations of single clusters show that axons from daughter neurons form a neuropile within the cluster of cell bodies. The neuropile increases in size and complexity for several hours, during which time chemical, and probably electrotonic, synapses form between neurites. Clear vesicles with diameters of about 35 nm and dense core vesicles with diameters of about 60 and 160 nm were detected. The development of the neuropile indicates that the prerequisite cell recognition phenomena were manifested during differentiation in vitro, and the complexity of the neuropile suggests it may have attained the capacity to process information.

Animals↗
PubMed1976

Ultrastructural differentiation during embryonic Drosophila myogenesis in vitro.

Cultures of embryonic Drosophila melanogaster cells were examined by electron microscopy and events in myogenesis were recorded. Thick and thin myofilaments, T-tubules and sarcoplasmic reticulum all appeared at about the same time, 10.5 hr. This was about 5 hr after the final division of myoblasts and about the time that muscle cells were elongating, aligning and fusing. Sarcoplasm typical of insect muscle was detected by 18.5 hr, as were myotendonal and tendocuticular junctions. Two populations of myocytes were detected, the cytoplasm of one more electron-dense than the other. The only previous report of myofibrilogenesis in invertebrate embryos had described novel mechanisms. In Drosophila embryonic material, however, the sequence of myofibrilogenesis resembled that in postembryonic insect or vertebrate material.

Animals↗
PubMed1975

Effect of peyote on human chromosomes. Cytogenetic study of the Huichol Indians of Northern Mexico.

Fify-seven Huichol Indians with a lifelong individual history and a 1,600-year cultural tradition of ingestion of peyote, a mescaline-containing cactus possessing hallucinogenic properties, were compared with 50 Huichol Indian controls and ten laboratory controls for effects on lymphocyte chromosomes. The frequency of abnormalities in the experimental and control groups did not differ significantly. Our results indicate that multigenerational ingestion of peyote is not associated with abnormalities in lymphocyte chromosomes.

Adolescent↗