THE REPLICATION OF BACTERIOPHAGE MS2. IV. RNA COMPONENTS SPECIFICALLY ASSOCIATED WITH INFECTION.
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Biomedical subjects
Publications and source records attributed to R L SINSHEIMER.
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The single-stranded DNA of coliphage X174 has been examined with the electron microscope by a modification of the protein-monolayer-adsorption technique. The molecules were found to be circular with a total length of 1.77 +/- 0.13 microns.
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Electron micrographs of surface films containing the replicative form of the DNA of bacteriophage, øX174 show ring structures, whose average contour length is 1.64 micro, which have the characteristic appearance of double-stranded DNA throughout most of their length.
When a preparation containing the DNA of the bacteriophage øX174 in its replicative form together with DNA from Escherichia coli is treated with exonucleases, the replicative form retains its characteristic physical properties, while the bacterial DNA is degraded. The evidence suggests that the mature replicative form exists as a closed ring.
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