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Biomedical subjects

R L Russell

Publications and source records attributed to R L Russell.

At least 37 records · Page 2Linked to original sources
PubMed1997

Association of a baculovirus-encoded protein with the capsid basal region.

An open reading frame homologous to AcMNPV ORF9 (ORF1629) was characterized in the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV). Sequence analysis indicated that the OpMNPV homolog (called ORF2) encoded a protein predicted to contain 545 amino acids with a molecular weight of 61 kDa. The first 80 amino acids did not have a counterpart in the AcMNPV homolog. The remainder of the ORF was poorly conserved with 29% amino acid identity overall with the AcMNPV ORF. However, the amino terminal 150 amino acids of AcMNPV ORF9 demonstrated about 45% amino acid sequence identity with OpMNPV ORF2 and conserved runs of proline residues were present in internal regions of both molecules. Transcriptional mapping indicated the ORF2 transcripts were initiated at a late promoter sequence, ATAAG, beginning about 24 hr p.i. These transcripts terminated near the 3' end of the ORF2 reading frame. Antibodies were produced against a fusion protein derived from the bacterial gene encoding the maltose binding protein and most of the ORF2 sequence. These antibodies reacted with a protein of 69 kDa on Western blots and the protein was found to be associated with virions isolated from both polyhedra and budded virus. The OpMNPV ORF2 antiserum also reacted with the AcMNPV ORF9 gene product. Immunoelectron microscopic analyses indicated that ORF2 was associated with the ends of the capsids which contain the basal structure. This end appears to be oriented away from both the virogenic stroma and membranes involved in intranuclear envelopment. In addition, as virions bud from the nucleus into the cytoplasm, this end also appears to be oriented away from the nuclear membrane.

Amino Acid Sequence↗
PubMed1997

Correspondence between DSM-III-R and DSM-IV attention-deficit/hyperactivity disorder.

OBJECTIVE: To evaluate the correspondence between DSM-III-R and DSM-IV definitions of attention-deficit/hyperactivity disorder (ADHD) in clinically referred children. Results of the field trials led to the hypothesis that there would be a strong correspondence between DSM-III-R and DSM-IV subtypes. METHOD: The sample consisted of all children and adolescents consecutively referred to a pediatric psychopharmacology clinic (N = 405). Children were comprehensively evaluated with structured diagnostic interviews assessing both DSM-III-R and DSM-IV ADHD. DSM-III-R symptoms were used to approximate DSM-IV subtypes. Kappa statistics and conditional probabilities were used to examine the correspondence between DSM-III-R and DSM-IV ADHD. RESULTS: Ninety-three percent of children who received a DSM-III-R diagnosis of ADHD also received a DSM-IV ADHD diagnosis. The kappa coefficient assessing the agreement between DSM-III-R and DSM-IV ADHD was .73 (z = 14.6, p < .0001). The kappa coefficient assessing the agreement between the DSM-III-R-approximated subtypes and the actual DSM-IV subtypes was .71 (z = 15, p < .0001). CONCLUSION: These results confirm previous findings and indicate that the change from DSM-III-R to DSM-IV results in minimal changes in case identification and provides support for diagnostic continuity between the two classification systems.

Adolescent↗
PubMed1996

Psychotherapy research in historical perspective. Implications for mental health care policy.

This article is a sketch of the historical development of the field of behavioral and non-behavioral therapy research. Four phases are characterized: (1) establishing scientific research (1927-1954), (2) searching for scientific rigor (1955-1969), (3) expansion and organization (1970-1983), and (4) consolidation and reformulation (1984-present). Continuities between and key developments within successive phases are outlined, with emphasis given to methodological innovations. The corroboration of select findings about process and outcome and the development of several critical discourses within the third and fourth phases have implications for the provision of mental health care and for policy discussions.

Health Policy↗
PubMed1996

People: the keys to profitability.

Various studies report that investment in people pays off two to one over other capital investments. Because business is fundamentally a matter of relations among human beings, training is essential for the development of the practice staff to effectively work together and give quality service to clients. Interpersonal skills are essential, as illustrated in the Gallop Poll organization discovery that most customer service in America is poor and that much of it is awful. This report enumerates five steps to aid in the development of the practice staff to be able to meet the expectations of the client so that each client visit is a pleasant, educational, and rewarding experience. The five steps are (1) developing rapport, (2) creating a professional presence, (3) focusing on getting and giving information, (4) showing how much you care, and (5) placing a high priority on staff training.

Animals↗
PubMed1996

Confirmatory P-technique analyses of therapist discourse: high-versus low-quality child therapy sessions.

From a sample of 35 child therapy sessions, 3 of the highest and 3 of the lowest quality sessions (HQS and LQS) were identified by independent ratings on the Loyola Child Psychotherapy Process Scales. Each of the 535 therapist-utterances in the HQS and the 372 therapist-utterances in the LQS were rated on 15 language interaction scales. Principal-components P-technique analyses were applied separately to the HQS and LQS, revealing 3 factors, Responsive Informing (RI), Initiatory Questioning (IQ), and Positive Regard (PR), that seemed to correspond across session types. Using confirmatory P-technique, a general measurement model with acceptable goodness-of-fit was determined for HQS. Two factors emerged: RI and IQ but not PR. Using a bootstrap procedure, random halves of the HQS were repeatedly sampled and compared via multigroup confirmatory factor analysis to create a reference distribution of the magnitudes of their difference chi-squares. Random resamples of HQS and LQS were then compared, resulting in a second reference distribution. Statistical tests and effect-size measures revealed replicable and large differences between sessions types.

Child↗
PubMed1996

Is childhood oppositional defiant disorder a precursor to adolescent conduct disorder? Findings from a four-year follow-up study of children with ADHD.

OBJECTIVE: To evaluate the overlap between attention-deficit hyperactivity disorder (ADHD) and oppositional defiant disorder (ODD), addressing whether ODD is subsyndromal form of conduct disorder (CD) and, if so, whether it is a precursor or prodrome syndrome of CD. METHOD: Assessments from multiple domains were used to examine 140 children with ADHD and 120 normal controls at baseline and 4 years later. RESULTS: Of children who had ADHD at baseline, 65% had comorbid ODD and 22% had CD. Among those with ODD, 32% had comorbid CD. All but one child with CD also had ODD that preceded the onset of CD by several years. ODD+CD children had more severe symptoms of ODD, more comorbid psychiatric disorders, lower Global Assessment of Functioning Scale scores, more bipolar disorder, and more abnormal Child Behavior Checklist clinical scale scores compared with ADHD children with non-CD ODD and those without ODD or CD. In addition, ODD without CD at baseline assessment in childhood did not increase the risk for CD at the 4-year follow-up, by midadolescence. CONCLUSIONS: Two subtypes of ODD associated with ADHD were identified: one that is prodromal to CD and another that is subsyndromal to CD but not likely to progress into CD in later years. These ODD subtypes have different correlates, course, and outcome.

Adolescent↗
PubMed1995

Interactional structures of therapist and client participation in adult psychotherapy: P technique and chronography.

In previous studies, the utterances of 6 clients and their therapists in early, middle, and late sessions were rated on 34 and 39 speech categories, respectively. P-technique analyses revealed 4 client and 4 therapist factors. For the present study, therapist and client utterances were assigned factor scores. A 17 x 17 correlation matrix was constructed, consisting of correlations across 4 lagged utterances (therapist-client-therapist-client), with each utterance represented by 4 factor scores and a score for the third from which the utterance was sampled. Principal-components analysis of this matrix revealed 4 therapist-client interaction factors: Mutual Therapeutic Engagement, Therapeutic Negotiation, Undirected Client Reminiscence, and Sustained Therapist Work. Unsuccessful cases deviated most from successful cases on at least 1 factor. Comparisons of interaction chronographs of episodes drawn from a successful and unsuccessful case revealed meaningful differences. Discussion highlights the power of P technique to reveal structures of psychotherapeutic discourse.

Adult↗
PubMed1995

The paradox of the contented working woman in intercollegiate athletics administration.

We investigated the paradox of the contented working woman among middle and first-line intercollegiate athletics administrators. In this paradox, women report lower salaries than men but express comparable job satisfaction. The sample comprised 143 women and 371 men in 106 National Collegiate Athletic Association (NCAA) Division I-A institutions. Job satisfaction was measured with the Job Descriptive Index (Smith, Kendall & Hulin, 1969) and Job in General (Ironson, Smith, Brannick, Gibson & Paul, 1989) scales. Analyses revealed the existence of the paradox: Women were paid significantly less, but were equally satisfied with their jobs. Neither gender was satisfied with promotion opportunities or pay, but both were highly satisfied with their jobs in general--another apparent paradox. The disproportionate number of male respondents raised questions about gender equity in athletics administration.

Administrative Personnel↗
PubMed1995

Introduction to the special section on multivariate psychotherapy process research: structure and change in the talking cure.

In response to the search for novel investigative strategies to apply in the study of psychotherapy processes, several investigators have turned to the use of factor-analytic methods of investigation. Four studies are presented that use such methods to investigate the structure of, and change in, aspects of the client and therapist discourse and symptoms over the course of treatments. The corpora of process data, culled from investigations taking place on three different continents, represent some of the largest samples of in-therapy behavior that have been analyzed in microanalytic detail. Providing both methodological advances and important substantive findings, the studies included were conducted to stimulate further use and development of factor-analytic methods to identify and track key processes over the course of psychotherapeutic treatment.

Humans↗
PubMed1994

Orgyia pseudotsugata baculovirus p10 and polyhedron envelope protein genes: analysis of their relative expression levels and role in polyhedron structure.

To investigate the regulation of p10 and polyhedron envelope protein (PEP) gene expression and their role in polyhedron development, Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis viruses lacking these genes were constructed. Recombinant viruses were produced, in which the p10 gene, the PEP gene or both genes were disrupted with the beta-glucuronidase (GUS) or beta-galactosidase (lacZ) genes. GUS activity under the control of the PEP protein promoter was observed later in infection and its maximal expression was less than 10% the level for p10 promoter-GUS constructs. Tissues from O. pseudotsugata larvae infected with these recombinants were examined by electron microscopy. Cells from insects infected with the p10- viruses lacked p10-associated fibrillar structures, but fragments of polyhedron envelope-like structures were observed on the surface of some polyhedra. Immunogold labelling of cells infected with the p10-GUS+ virus with an antibody directed against PEP showed that the PEP was concentrated at the surface of polyhedra. Although polyhedra produced by p10 and PEP gene deletion mutants demonstrated what appeared to be a polyhedron envelope by transmission electron microscopy, scanning electron microscopy showed that they had irregular, pitted surfaces that were different from wild-type polyhedra. These data suggested that both p10 and PEP are important for the proper formation of the periphery of polyhedra.

Animals↗
PubMed1994

Reduced pressure in extended coverage anti-G-trousers with assisted pressure breathing.

Six Swedish Air Force pilots and two Armstrong Laboratory subjects volunteered for this study to determine whether reduced pressure in extended coverage anti-G trousers (ECT), in combination with positive pressure breathing for G protection, affected +Gz tolerance. Group mean gradual onset run (GOR) time for the low pressure schedule (0.8 psig.G-1) was significantly different from the other two conditions (1.1, 1.5 psig.G-1)--84.1, 95.6, and 94.1 s, respectively. Group mean simulated aerial combat maneuver (SACM) times statistically differed between the low and middle pressure rates compared to the highest pressure schedule--147.6, 164.8 and 232.3 s, respectively. SACM heart rate (HR) responses for the +9 Gz plateaus were not statistically different among conditions. However, +5 Gz HR's were lower for the highest pressure condition. Similarities were identified between the middle and high pressurization conditions for GOR. However, reducing pressure in the ECT during SACM exposures was not supported by these data.

Gravity Suits↗
PubMed1993

A baculovirus encoded 16-kDa glycoprotein localizes near the nuclear membrane of infected cells.

An open reading frame (ORF 2) located upstream of the polyhedron envelope protein gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) was cloned in frame into a trpE expression vector. The fusion protein produced by this construct was used for the production of a monospecific antiserum. Western blot analysis of OpMNPV-infected Lymantria dispar cells detected a 16-kDa protein at 24 hr postinfection. The 16-kDa protein was determined to be N-glycosylated by tunicamycin treatment of infected cells. Immunofluorescence microscopy localized the 16-kDa protein to foci of intense cytoplasmic staining near the nuclear membrane. Immunoelectron microscopy indicated that the 16-kDa protein is associated with lamellar-like structures peripheral to the nuclear membrane and with envelopes of virus that have budded into the cytoplasm. The 16-kDa protein was not associated with extracellular budded or polyhedron-derived virions.

Animals↗
PubMed1993

A 25-kDa protein is associated with the envelopes of occluded baculovirus virions.

Antiserum produced against preoccluded virions of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV) was used to screen an OpMNPV lambda gt11 expression library. One of the immunoreactive clones contained an insert that hybridized to a portion of the OpMNPV HindIII-P fragment. A 2-kb region of HindIII-P was sequenced and found to contain three open reading frames, each preceded by a late promoter element. The insert from the lambda gt11 clone was derived from the third open reading frame, which encodes a predicted protein of 25 kDa. The lambda gt11 insert was cloned into a pMALcR1 bacterial expression vector and the fusion protein was expressed, isolated, and used for antibody production. This antiserum detected a doublet of approximately 25 kDa on Western blots of a time course of OpMNPV-infected Lymantria dispar cells. The protein was first detected at low concentration by 18 hr p.i.; by 36 hr p.i., the protein concentration had increased significantly and remained at this level for the duration of the time course. Similar results were seen on Western blots of Autographa californica MNPV (AcMNPV)-infected Spodoptera frugiperda cells. No evidence of O- or N-linked glycosylation of the OpMNPV p25 was found. Immunoelectron microscopy showed that p25 was present in the nuclei of OpMNPV-infected cells and localized to the envelopes surrounding polyhedron-derived virions.

Amino Acid Sequence↗
PubMed1993

Earvin "Magic" Johnson's HIV serostatus disclosure: effects on men's perceptions of AIDS.

The effects of celebrity self-disclosure of human immunodeficiency virus (HIV) seropositivity on perceptions of HIV and Acquired Immunodeficiency Syndrome (AIDS) were investigated. AIDS-related interest and knowledge measures were collected from 468 men before and after basketball star Earvin "Magic" Johnson's self-disclosure of HIV seropositivity. Increased interest in AIDS paralleled media coverage of the announcement, with the most substantial effects occurring within 2 weeks. Perceived impact of the disclosure was greatest among African-American men and men who had not previously known someone with HIV-AIDS. Celebrity self-disclosure appears to affect perceptions through mechanisms similar to those involved in personally knowing someone infected with HIV.

Acquired Immunodeficiency Syndrome↗