Biomedical subjects
Publications and source records attributed to R L Prestidge.
Buffer electrofocusing, utilizing a mixture of low-molecular-weight buffer compounds to establish a stable linear pH gradient covering the range 3.5-6.0 on granulated gel media, has been employed in the purification of Interleukin I with good recovery of biological activity. The difficulties often experienced in the removal of commercial synthetic ampholytes after conventional preparative electrofocusing are avoided in this method.
The murine macrophage-derived cell line P388D1 is commonly used for the production of Interleukin 1 (IL1) and other macrophage products. In order to circumvent the tendency of this cell line to undergo selection for a non-adherent subpopulation of cells which does not produce IL1, we have grown P388D1 cells attached to microcarrier beads. IL1 produced by these cells is indistinguishable from that elaborated by cells grown in monolayer or suspension culture. Moreover, several volumes of conditioned medium can be produced from one batch of cells suitable for the large-scale purification of this mediator.
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Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).
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