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Biomedical subjects

R L Nichols

Publications and source records attributed to R L Nichols.

At least 145 records · Page 8Linked to original sources
PubMed1970

Antibody type specificity to trachoma in eye secretions of saudi arab children.

The presence of trachoma antibody in eye secretions of Saudi Arab children appears to be a direct response to the infecting organism. In 35 of 36 cases, the trachoma antibody type found in eye secretions was identical to the strain type isolated concurrently. Strain types were identified by use of donkey hyperimmune sera prepared against types 1, 1b, and 2. The specificity of the sera was enhanced by absorption with trachoma antigens of heterologous type. Similarly, antibody types were determined by absorbing samples of eye secretions with types 1, 1b, and 2. Complete absorption of trachoma antibody activity was achieved only by the antigen type homologous to the patient's own strain. In one instance, antibody could not be typed. No change in antibody or strain type was seen in four children examined twice during a 3- to 6-month period. Typing of trachoma antibody from eye secretions has potential as an immunological and epidemiological tool.

Journal Article↗
PubMed1970

Immunoglobulins, secretory component, and transferrin in eye secretions of infants in regions with and without endemic trachoma.

Eye secretions were collected from Boston and Saudi Arab infants between 1 day and 6 months of age. These secretions were then tested with monospecific antisera in double diffusion and immunoelectrophoresis for the presence of immunoglobulin (Ig)A, IgM, IgG, secretory component, and transferrin. (The term "secretory component" has been chosen by the World Health Organization to replace the terms "secretory piece" and "transport piece.") Eye secretions of all newborns contained secretory component. IgA was found attached to the secretory component in samples from some infants as young as 8 days of age and in samples from most infants over 3 weeks of age. IgG and transferrin were found in most samples regardless of the age of the infant. The prevalence of the respective proteins in the eye secretions of Boston infants was found to be similar to their prevalence in the eye secretions of Saudi Arab infants, including four who developed trachoma during the study. Specific antibody to trachoma was demonstrated in the eye secretions of those infants with active trachoma, and the immunoglobulin class was determined.

Journal Article↗