Biomedical subjects
Publications and source records attributed to R L Morgan.
This report is the first description of a secondary ovarian liposarcoma. The primary growth was in the mediastinum. The growth rate of the ovarian tumor was very rapid, but the patient died of cardiopulmonary causes. The mode of spread is discussed, and it probably occurred by transcelomic migration and surface implantation.
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Investigations of the physiological disposition of cyanide during thiosulfate therapy has necessitated the development of a convenient and sensitive method for cyanide analysis. A fluorometric method involving the catalytic conversion of pyridoxal to 4-pyridoxylactone was adapted for use with biological fluids by employing microdiffusion analysis. The presence of the cyanide antagonist, sodium thiosulfate, interferes with the formation of the fluorophore. In order to circumvent this interference, the pH of the diffusion media was altered to selectively diffuse cyanide. After testing various acidifying agents, an acetate buffer (pH = 5.2) was determined to be satisfactory. The fluorometric method was then correlated with the classical colorimetric procedure by an in vivo study. Blood from mice treated with sodium nitrite and sodium thiosulfate, prior to receiving potassium cyanide, was analyzed by both procedures and no significant difference was demonstrated between the results of the two methods of analysis.
1,1'-Trimethylene [1,1'-14C]bis(4-aldoximinopyridinium) ion (TMB-4) was synthesized and its metabolic disposition was investigated in vivo. Rats were administered multiple doses of TMB-4 (25 mg/kg) by the intraperitoneal route and urine was collected over a 48-hr period. Approximately 98% of the administered radioactive dose could be accounted for in the urine during that time. A urinary metabolite, trimethylene-1-(4-aldoximinopyridinium)-1'-(4-carboxamidopyridinium) ion (TACARB), was isolated by ethanol extraction, charcoal adsorption chromatography, and ion-exchange chromatography. The metabolite was then characterized by comparing its spectral, chromatographic, and electrophoretic properties with those of authentic TACARB ion. Possible reaction mechanisms involved in the biochemical pathways for the formation of this metabolite from TMB-4 are discussed.
Eight-week-old chickens were inoculated with one of two exotic viruses to determine the effect of composting on virus survival. Group 1 chickens were inoculated with highly pathogenic avian influenza (HPAI) virus via the caudal thoracic air sac. Group 2 chickens were inoculated with the adenovirus that causes egg drop syndrome-76 (EDS-76) by the oral route. Five days after inoculation, lung, trachea, and air sacs for HPAI and spleen, cecal tonsils, and bursa of Fabricius for EDS-76 were collected and composted with poultry carcasses. At the end of the first 10 days of composting, virus-isolation efforts showed that the HPAI virus had been inactivated, and only 1 of 20 tissue samples yielded the adenovirus of EDS-76. The viruses of HPAI and EDS-76 were completely inactivated at the end of the second 10-day period of the two-stage composting process. Control tissues collected at necropsy and frozen at -70 C for virus isolation were all positive for virus.