Glomerular epithelial protein 1 (GLEPP1) is a podocyte receptor membrane protein tyrosine phosphatase located on the apical cell membrane of visceral glomerular epithelial cell (VGEC) foot processes. Double label immunofluorescence, immunoelectron microscopy, and peroxidase immunohistochemistry were used to map the GLEPP1 distribution in the developing glomerulus and in minimal-change nephropathy (MCN), congenital nephrotic syndrome of the Finnish type, and focal-segmental glomerulosclerosis (FSGS). In MCN GLEPP1 was shifted away from the glomerular basement membrane on the apical cell membrane of effaced foot processes. These data are compatible with the previously suggested concept that MCN can be considered a form of dedifferentiation of the podocyte phenotype. Similarly, changes seen in congenital nephrotic syndrome of the Finnish type can be considered a consequence of failure to complete normal podocyte development. In FSGS glomeruli GLEPP1 was frequently absent from VGECs, even when no sclerosis was detectable in that glomerulus. Therefore, in FSGS, VGECs may lose GLEPP1, and this loss appears to occur in the absence of scarring and may, therefore, precede the scarring process. We speculate that a changed VGEC phenotype that does not express GLEPP1 might have properties similar to the early undifferentiated VGEC developmental phenotype. GLEPP1 distribution pattern and absence from glomeruli of individuals with nephrotic syndrome may, therefore, represent a useful phenotypic marker.

Animals↗

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[The transsigmoidal ureterosigmoidostomy (sigmoid-rectum-bladder):surgical technique and determination of total potassium].

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Acidosis↗

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[Verbal behavior and communication experience of psychosomatic patients in the first psychoanalytic interview in connection with the concept of "pensée opératoire'].

This pilot-study was concerned with differences in speech behaviour and communication as experienced in psychoanalytically oriented initial interviews. In line with the ideas of Marty, Fain, de M'Uzan, David and Sami-Ali, 38 patients were divided into 3 diagnostic groups according to the extent of "pensée opératoire" and the level of functioning of the psychic sphere in each case. The communication experienced was recorded with the help of a communication questionnaire developed in Giessen. The speech behaviour for the interview as a whole and in its development over time was registered automatically and record in 29 categories by the Giessen Speech Analyzer. The following results are reported: --The two groups with more extensive "pensée opératoire" (A1, A2) differed from the character-neurotic patients in 13 of the 29 speech categories registered. The differences between groups A1 and A2 were not significant. --The communication as experienced by the patients did not differ between groups, but the therapist experienced the patients from the 3 diagnostic groups differently. The information gathered from the therapist in the communication questionnaire agreed by and large with the data collected automatically. --For the development of the interview over time, characteristic changes in the frequency of various speech categories were found but these were in the main unrelated to the diagnostic groupings.

Adult↗