Search PubMed⌕ Search

Biomedical subjects

R Kirsch

Publications and source records attributed to R Kirsch.

At least 73 records · Page 4Linked to original sources
PubMed1975

Laboratory investigations in sheep with a new anthelmintic.

The efficacy of fenbendazole against the following gastrointestinal nematodes was tested in experimentally infected lambs: O circumcincta, H contortus, T colubriformis and N filicollis. As low a dose as 0.5 mg per kg reduced the egg output of a patent infection with H contortus and T colubriformis by 85 per cent to 100 per cent. A dose of 3.5 mg per kg has a 100 per cent effect on three or 10-day-old stages of H contortus and one of more than 99 per cent or 100 per cent T colubriformis. The same dose reduces seven-day-old stages of O circumcincta and N filicollis by more than 94 per cent or 100 per cent, while 10 mg per kg produces a 100 per cent elimination of seven-day-old stages of O circumcincta. In field trials and efficacy of more than 99 per cent was determined after oral dosage with 5.0 mg per kg against Ostertagia, Haemonchus, Trichostrongylus, Cooperia, Nematodirus and Chabertia. Fenbendazole has a wide therapeutic-toxic dose ratio. Discoloration of the wool does not occur. Fenbendazole can be administered as a drench or with the feed.

Animals↗
PubMed1975

[Structural modifications of the esophagus linked to osmoregulation in eels].

In comparison with the fresh-water Eel (Anguilla anguilla L.), the oesophagus of the sea-water Eel displays an almost complete regression of the multilayered epithelium and consequently the disappearance of mucous cells. In contrast, a large development of a columnaŕ epithelium is associated with a tremendous proliferation of the subepithelial vascularization. These modifications enable one to interpret the changes in ionic permeability of the oesophagus during transfer from freshwater to seawater.

Animals↗
PubMed1975

[Experimental investigations on the effectiveness of fenbendazole in parasitic helminths of the stomach, intestines and lung of cattle (author's transl)].

Helminth-free bulls were injected artificially with Ostertagia ostertagi (120 000 L3), Cooperia oncophora (240000 L3), Haemonchus spec. (1 000 L3) and/or Dictyocaulus viviparus (60 L3/kg) orally or intraruminally. The animals were treated with fenbendazole during the prepatent period or after having reached maturity. The effect of 5 mg fenbendazole/kg p.o. on 3-d, 10-d- and adult stages of Ostertagia ostertagi, Haemonchus spec. and Cooperia oncophora ranges between less than 94% and 100%. Particularly noticeable was the effect on 10-d-old Ostertagia ostertagi with less than 94%. 5 mg/kg fenbendazole orally removed nearly 100% of 6-d-, 13-d-, and adult stages of Dictyocaulus viviparus. Fenbendazole may be administered as a drench or as medicated feed. The safety of the compound permits the administration of excessively high doses without clinical signs.

Animals↗
PubMed1975

Structural and functional studies of ligandin, a major renal organic anion-binding protein.

Sephadex gel filtration of the 1000,000 g supernate of homogenates of rat kidney revealed binding of various organic anions (penicillin, Bromsulphalein [BSP], bilirubin, phenolsulfonphthalein [PSP], phlorizin, glutathione [GSH], p-amino hippurate (PAH), probenecid, conjugated bilirubin, and BSP-GSH) to a nonalbumin-containing protein fraction (Y), which precipated on addition of monospecific anti-rat liver ligandin (Y protein)-IgG, but not control IgG. Quantitatively similar organic anion binding was observed in vivo after injection of BSP, BSP-GSH, phlorizin, probenecid, conjugated bilirubin, PAH, or penicillin. The binding protein was purified to apparent homogeneity and is a basic protein (pI 8.9) of 44,000 daltons with two apparently identical subunits of 22,000 daltons. Monospecific antibody was produced against the renal protein. The results of binding studies in vivo and in vitro and phsicochemical, immunologic, structural, and binding site investigations indicate that the renal protein is identical to hepatic ligandin. Immunofluorescent studies utilizing anti-ligandin IgG previously localized ligandin in the kidney to all proximal tubular cells. By quantitative radial immunodiffusion, the concentration of renal ligandin was 31.2 plus or minus 2.2 mug/mg supernatant protein and was increased 160% above basal values by pretreatment of rats with tetrachloro-dibenzo-p-dioxin. Pretreatment with phenobarbital, DDT, or pregnene-16alpha-carbonitrile did not increase renal ligandin concentration but doubled hepatic ligandin concentration. Circular dichroism studies of renal ligandin revealed percent helical structure similar to hepatic ligandin and primary association contrasts were derived for BSP (10-6 M-1) and PAH, probenecid, and penicillin (10-3 M-1). Administration of BSP or probenecid simultaneously with [C14] penicillin resulted in increased plasma retention and reduced kidney and urinary bladder content of [14C] penicillin and a correlation coefficient of -0.8 between total kidney/plasma radioactivity and percent of protein-bound radioactivity bound to ligandin in the kidney. These studies indicate that renal and hepatic ligandin are identical. Their response to drugs and chemicals varies. Competitive binding between several organic anions for ligandin correlated with their renal uptake from plasma, which suggests that ligandin may function in the proximal tubular cell as a component of the renal organic anion transport system.

Amino Acids↗
PubMed1975

Laboratory investigations on pigs with the new anthelmintic fenbendazole.

The efficacy of fenbendazole was investigated in piglets infected artificially with Hyostrongylus rubidus and Oesophagostomum spp. After administration of 3.5 mg/kg, five-day-old stages of H rubidus were reduced by 72.5 per cent; an effect of 78.4 per cent, 96.0 per cent and 100 per cent was achieved against five-day-old, 16-day-old and 42-day-old stages, respectively, of H rubidus using a dose of 5 mg/kg. A 55 per cent effect was obtained against five-day-old stages after the administration of 3.5 mg/kg. A dose of 5 mg/kg reduced five-day-old, 16-day-old and 42-day-old stages of Oesophagotomum spp by 72.6 per cent, 44 per cent and 100 per cent respectively.

Animals↗
PubMed1975

[Water balance in the european eel (Anguilla anguilla L.). Role of the oesophagus in the utilisation of drinking water and a study of the osmotic permeability of the gills (author's transl)].

10 New experimental devices are described which allow chonic measurements of drinking rate and osmotic gill permeability in the eel. 20 The oesophagus of the seawater (SW) silver eel plays a role in osmoregulation. It decreases the concentration of Cl- and Na+ of the ingested SW without losing water in the serosal to mucosal direction. This allows for immediate water absorption in the intestine and decreases the quantity of ions actively absorbed by the intestine. In the freshwater (FW) silver eel, the oesophagus is impermeable to water, Cl- and Na+. The ionic impermeability exists only in the serosal to mucosal direction. A mucosal to serosal permeability to Cl- and Na+ exists in the FW oesophagus receiving hypertonic drinking water, this promotes seawater adaptation. 30 The osmotic gill permeability, measured in vivo in the silver eel, is very low in FW and decreases slightly in SW. Thus, the silver eel has an osmotic gill permeability preadapted to SW life. The kinetics of FW to SW adaptation are described.

Adaptation, Physiological↗