Search PubMed⌕ Search

Biomedical subjects

R Kern

Publications and source records attributed to R Kern.

At least 37 records · Page 2Linked to original sources

Optomotor course control in flies with largely asymmetric visual input.

We have studied freely flying and walking flies as well as flies flying in a flight simulator in order to discover how functionally blinding one of the eyes affects the fly's ability to move straight. It is hard to tell just by observing the animals' movements whether they have been deprived of vision in one eye. Statistical analysis is need to show that there are differences in the locomotory paths of monocular and binocular flies: monocular flies tend to turn slightly towards the side of the seeing eye. It is possible that the superimposed translational and rotational optic flow fields, generated on the trajectory of monocular flies, sum to zero net flow. This overall flow over the retina of the open eye might lead to a state of optomotor equilibrium.

Animals↗

Neuronal representation of optic flow experienced by unilaterally blinded flies on their mean walking trajectories.

Asymmetries in the optic flow on both eyes may indicate an unintended turn of an animal and evoke compensatory optomotor responses. On a straight path in an evenly structured environment, the optic flow on both eyes is balanced corresponding to a state of optomotor equilibrium. When one eye is occluded an optomotor equilibrium is expected to be reached on a curved path provided that the translatory optic flow component is cancelled by a superimposed rotation. This hypothesis is tested by analysing how the HSE cell, a constituent element of the fly's optomotor system, represents optic flow in behavioural situations. The optic flow as seen on the average trajectory of freely walking monocular flies is reconstructed. This optic flow is used as stimulus of the HSE cell in electrophysiological experiments and as input of a model of the fly's optomotor system. The responses of the HSE cell and of the model fluctuate around the resting potential. On average, they are much smaller than the responses evoked by optic flow experienced on a straight path. These results corroborate the hypothesis that the mean trajectory of monocular flies corresponds to a path of optomotor equilibrium.

Animals↗

Competition between the replication initiator DnaA and the sequestration factor SeqA for binding to the hemimethylated chromosomal origin of E. coli in vitro.

BACKGROUND: Following replication initiation, the replication origin (oriC) in Escherichia coli enters a hemimethylated state at Dam methylation sites which are recognized by the SeqA protein. SeqA binds preferentially to hemimethylated GATC sequences of DNA in vitro. SeqA is essential for the synchronous initiation of chromosome replication from oriC copies in vivo. RESULTS: We show that: (i) purified SeqA binds AT-rich and 13-mers regions and two DnaA boxes, R1 and M, of hemimethylated oriC. (ii) SeqA inhibits the in vitro replication of a hemimethylated oriC plasmid more efficiently than the fully methylated, (iii) SeqA inhibits competitive binding of DnaA protein to the regions of the hemimethylated oriC plasmid, explaining the mechanism of its inhibitory effect. The inhibition of DnaA binding by SeqA also occurs efficiently on a small hemimethylated oriC fragment containing both R1 and M DnaA boxes, but not the 13-mer region. CONCLUSIONS: SeqA binds strongly the long region from the AT-rich region to the M DnaA box of the hemimethylated oriC DNA and releases DnaA molecules from the long region.

AT Rich Sequence↗

Arterial gas embolism after decompression: correlation with right-to-left shunting.

Paradoxical gas embolism is a possible cause of neurologic sequelae after decompression in divers. The authors detected arterial bubbles after decompression from chamber dives in two of six divers using transcranial Doppler sonography (TCD). Arterial bubbles correlated with the size of right-to-left shunting as diagnosed by contrast TCD. The pathway of spontaneous paradoxical embolism was tracked for the first time, supporting the concept of paradoxical gas embolism as a cause of early neurologic sequelae after decompression in at-risk divers.

Adult↗

Application of pharmacokinetics to electron-beam tomography of the abdomen.

RATIONALE AND OBJECTIVES: The purpose of this study was to evaluate the pharmacokinetics of abdominal time-attenuation curves obtained at electron-beam tomography. MATERIALS AND METHODS: Computed tomographic enhancement data of the aorta, portal vein, vena cava, liver, spleen, and pancreas were obtained in 25 patients after injection of 50 mL of contrast medium. These data were used to calculate pharmacokinetic parameters such as half-lives, mean residence times, and areas under the curve with a computer program. RESULTS: Maximal enhancement was observed in the aorta 24 seconds +/- 5 (mean +/- standard deviation) after starting the injection of contrast medium (178 HU +/- 56), in the portal vein after 42 seconds +/- 14 (60 HU +/- 17), in the vena cava after 35 seconds +/- 7 (66 HU +/- 23), in the liver after 58 seconds +/- 15 (24 HU +/- 6), in the spleen after 35 seconds +/- 12 (42 HU +/- 16), and in the pancreas after 39 seconds +/- 15 (42 HU +/- 10). Half-lives of the last phase observed were 108 seconds +/- 123 in the aorta, 33 seconds +/- 30 in the portal vein, 49 seconds +/- 40 in the vena cava, 50 seconds +/- 54 in the liver, 62 seconds +/- 33 in the spleen, and 22 seconds +/- 27 in the pancreas. The computer program allowed for excellent fitting curves to the measured attenuation values and for subsequent calculation of pharmacokinetic parameters. New dosage regimens also could be simulated successfully. CONCLUSION: The pharmacokinetic parameters evaluated might be useful in the optimization of dosing and scanning parameters of the abdomen for ultrafast and helical CT.

Aorta, Abdominal↗

Hemi-methylated oriC DNA binding activity found in non-specific acid phosphatase.

The lacZ-hobH fusion clone, containing an Escherichia coli DNA segment located at 92 min on the chromosomal map, was screened as a producer of E. coli oriC hemi-methylated binding activity. We have purified the protein encoded by this locus to near homogeneity. The protein corresponds to the monomeric form of a non-specific acid phosphatase (NAP) whose gene has been designated aphA. oriC DNA footprinting experiments showed protection of hemi-methylated probe by partially purified NAP, but not by purified preparations. Yet, gel retardation experiments with an oriC oligonucleotide demonstrated DNA binding activity of purified NAP in the presence of Mg2+. This experiment also showed an increased affinity of the protein for the hemi-methylated probe compared with the fully or unmethylated form. Indirect immunofluorescene microscopy revealed the existence of discrete NAP foci at mid-cell in cells with two nucleoids, but at cell poles in those with one nucleoid.

Acid Phosphatase↗

Stereolithography versus milled three-dimensional models: comparison of production method, indication, and accuracy.

Computed tomography (CT) based three-dimensional (3-D) lifelike models have proved to be of great value, especially in craniomaxillofacial surgery. They improve and facilitate diagnosis, therapeutic planning, model operations, and definitive treatment in tumor surgery, traumatology, dysgnathia, alveolar atrophy, and congenital and asymmetrical malformations. From 1988 to 1998, 760 stereolithography (STL) and milled 3-D models were employed in our department. These two production methods have become the msot commonly used approaches, and the question as to which is preferable is the subject of controversy in the literature. Using two test models the STL and the milling method were compared with regard to production method and accuracy, and the resulting differences in indications for their use are discussed. The limiting factor for increased accuracy is the distance between each pair of CT scans. Milled models show a higher precision compared to STL models within the CT-scan plane, but the more the measurements deviate from this plane (becoming oblique) the more inaccurate the data becomes. STL exhibits greater deviations, but the inaccuracy is randomly distributed. The mean variation from the original was 0.81 mm for STL and 0.54 mm for milled models. Although 90% of the milled and 80% of the STL values are within a deviation of +/- 1 mm, it should be noted that maximum deviations of 3.15 mm in milled models and 2 mm in STL have been shown. Both methods are sufficiently accurate for clinical use. In standard cases involving the upper and lower jaw, malar bone, orbita, and calvaria the shorter production time and lower costs make milled models preferable. In special cases, in which hollows and fine structures play a major role (e.g., those involving the skull base, paranasal sinuses, inner ear, and mandibular canal), STL is indicated.

Computer Simulation↗

Visual position stabilization in the hummingbird hawk moth, Macroglossum stellatarum L. I. Behavioural analysis.

Optomotor responses of freely flying hawk moths, Macroglossum stellatarum, were characterized while the animals were hovering in front of and feeding on a dummy flower. Compensatory translational and rotational movements of the hawk moth were elicited by vertical grating patterns moving horizontally, mimicking imposed rotational and translational displacements of the animal in the horizontal plane. Oscillatory translational and rotational pattern motion leads to compensatory responses that peak in the frequency range between 2 Hz and 4 Hz. The control systems mediating the translational and rotational components of the optomotor response do not seem to influence each other. The system mediating translational responses is more sensitive in the fronto-lateral part of the visual field than in the lateral part; the opposite is true for the rotational system. The sensitivity of the translational system does not change along the vertical, whereas the rotational system is much more sensitive to motion in the dorsal than in the ventral part of the visual field. These sensitivity gradients may reflect an adaptation to the specific requirements of position stabilization in front of flowers during feeding.

Animals↗

Visual position stabilization in the hummingbird hawk moth, Macroglossum stellatarum L. II. Electrophysiological analysis of neurons sensitive to wide-field image motion.

Response properties of neurons in the cervical connectives of the hummingbird hawk moth, Macroglossum stellatarum L., were determined. All neurons described in this account respond directionally selectively to motion in large parts of the visual field of either eye. They respond maximally to bilateral stimulation, preferring either motion as induced on the eyes during translatory movements of the animal or when it turns around one of its body axes. Cells most sensitive to rotational motion either respond best to rotation of the patterns around the vertical axis of the animal or around its longitudinal body axis. Neurons most sensitive to translational pattern motion respond best to either simulated translations of the animal along its vertical or along an oblique axis. Most types of neurons respond tonically and do not habituate. The sensitivity to motion stimuli is not evenly distributed within the receptive field of any investigated neuron. Part of these neurons might play a role in visual position and course stabilization.

Animals↗

[Stereolithography models vs. milled 3D models. Production, indications, accuracy].

Anatomic, life-like, three-dimensional models have a definite place in cranio-maxillofacial surgery. Our experience with 541 computer tomographic (CT)-based three-dimensional (3D)-models for diagnostic purposes, preoperative planning and model operations in our department was gained using stereolithographic and milled models. The question of which production method is preferable is a matter of controversy in the literature. Both methods are based on CT and magnetic resonance imaging data, but the differences in production give rise to specific advantages and disadvantages. For comparison we scanned two measurement models, fabricated milled and stereolithographic models, and analyzed the differences concerning accuracy and shape of specific structures. The scan distance (feed) is the limiting factor for the accuracy for both methods. Milled models show the highest precision in the plane of CT scanning--the more oblique the measurements became to this plane, the greater the decrease in accuracy. Hollows and undercuts can only be produced by splitting the model. Stereolithographic models show higher deviations with randomly distributed errors. The mean deviation was 0.81 mm on stereolithography and 0.54 mm on milled models. The accuracy of both methods is sufficient for clinical use. In routine cases the milling method seems to be superior because of shorter production time and lower costs. In special cases, where hollows and fine structures play a major role, stereolithography is the method of choice.

Humans↗

Increased expression of a hemimethylated oriC binding protein, SeqA, in an aphA mutant.

In Escherichia coli, the origin of DNA replication, oriC, becomes transiently hemimethylated at the GATC sequences immediately after initiation of replication and this hemimethylated state is prolonged because of its sequestration by a fraction of outer membrane. This sequestration is dependent on a hemimethylated oriC binding protein such as SeqA. We previously isolated a clone of phage lambda gt11 called hobH, producing a LacZ fusion protein which recognizes hemimethylated oriC DNA. Very recently, Thaller et al. (FEMS Microbiol. Lett. 146 (1997) 191-198) found that the same DNA segment encodes a non-specific acid phosphatase, and named the gene aphA. We show here that the interruption of the aphA reading frame by kanamycin resistance gene insertion, abolishes acid phosphatase (NAP) activity. Interestingly, in the membrane of the null mutant, the amount of SeqA protein is about six times higher than that in the parental strain, suggesting the existence of a regulatory mechanism between SeqA and NAP expression.

Acid Phosphatase↗

[Quantitative determination of left ventricular myocardial perfusion with electron beam computerized tomography].

Myocardial perfusion is one of the most important functional parameters of the heart. Presently various indirect methods are used to determine coronary blood flow or myocardial perfusion as inertgas-, thermodilution-, Doppler catheter- and radiopharmacological techniques. Electron-beam-computed-tomographical technology is able to perform CT data acquisition with a very short exposure time of 50 ms. Using this method it is not only possible to determine left ventricular volumes but also to measure myocardial perfusion in ml/100 g/min. The measurement of the left myocardial perfusion is performed using the short axis view. This position is obtained by moving the table 25 degrees to the patient's right and 15 degrees caudally. To determine the position of the left ventricle, a localization scan is obtained in multi-slice-mode using all for target-rings, thus obtaining 8 tomographic levels over 68 mm (each tomographic level having a slice thickness of 7 mm, with an interslice gap of 4 mm between each two adjacent tomographic levels). In this short axis position, using the multi slice flow mode with 3 target-rings and after administration of 50 ml of contrast medium intravenously with a flow of 3 ml/s, 6 tomographic levels are imaged. Each tomographic level is obtained 13 times at 80% of the R-R-interval at each 2 or 3 heart beat (ECG-gated). The left ventricular myocardial contrast enhancement is measured by drawing manually the outline of the left ventricular myocardium using time-density-software of the Imatron workstation. For calculation of the myocardial perfusion the so-called "slope method" is used and the results are expressed as the maximum slope of enhancement of the myocardium divided by the difference of the precontrast and peak CT-value in the left ventricle. The global myocardial perfusion is calculated as a mean of all evaluated tomographic levels. In this study left ventricular volumes as enddiastolic volume endsystolic volume and stroke volume were measured and ejection fraction and cardiac output calculated. The measurements were performed in the log axis view. This view is obtained by moving the table 15 degrees to the patients left in a horizontal position. In this long axis position 6 tomographic levels are imaged using the multi-slice-cine-mode with 3 target-rings after administration of 50 ml of contrast medium intravenously with a flow of 3 ml/s. Each tomographic level is obtained 13 times starting at 0% of the R-R-interval (ECG-triggering). The exposure time is 50 ms with an interscan time delay of 8 ms. In 9 studied patients of whom one had 3 significant coronary artery stenotic lesions (> 50%), 2 patients had each 2 non significant stenotic lesions (< 50%) and 6 revealed nearly normal coronary angiograms. The mean global myocardial perfusion was 70 ml/100 g/min (min.32 and max. 116 ml/100 g/min). This mean value of 70 ml/100 g/min is reflecting 5% of the cardiac output supposing that the mean heart weight of these patients was 300 g. In this study the mean of the left ventricular muscle mass determined by the use of EBCT was 130 g. A comparative evaluation of coronary angiographic findings in these patients with the measured myocardial perfusion values revealed, that is not sufficient to look only at the absolute values of the measured myocardial perfusion. Furthermore it seems to be necessary to interpret these perfusion values with respect to the calculated cardiac output. Additional studies of well defined patients groups are necessary to determine normal values of myocardial perfusion at rest in patients with and without coronary artery disease. This seems to be important as comparative analysis of myocardial scintigraphic and EBCT-studies is difficult because of methodical inherent differences. The results of this study suggest that despite the presence of some beam hardening artifacts it is possible to measure myocardial perfusion using EBCT in patients with suspected coronary artery disease in the

Aged↗

[Electron beam computerized tomography (EBCT) of the heart].

Using electron-beam computed tomography (EBCT) with short exposure times of 100 or 50 ms and the capability of acquiring up to 2 x 17 images/s it is possible to study most of the important morphological and functional determinants of the heart. Various examples of studies in acute and chronic cardiac diseases are shown to demonstrate the use of EBCT to determine quantitatively left ventricular volumes (ml), myocardial mass (g), wall thickness changes over the cardiac cycle (mm/s), myocardial perfusion (ml/ 100 g/min) and the extent of coronary calcification (calcium score) and qualitatively the state of the proximal 4-6 cm of the subepicardial coronary arteries. The knowledge of these determinants seems very useful in excluding cardiac dysfunction, in the early recognition of cardiac disease and in the evaluation of the haemodynamic severity of coronary artery stenotic lesions. Further interdisciplinary studies are necessary to assess the clinical validity of these cardiac determinants, especially myocardial perfusion, using this advanced CT technology.

Aged↗

Edge detection by landing honeybees: behavioural analysis and model simulations of the underlying mechanism.

The mechanism of edge detection in the honeybee was investigated by examining the effects of combining different kinds of visual cues that define an edge. Free-flying bees were trained to land at three different types of edges which were defined by texture and relative motion cues either in isolation or in combination with each other. Bees are able to detect and land at the three types of edges, but do so with different frequencies. In contrast to the naive expectation that edges jointly defined by two cues can be detected better than those defined by a single cue in isolation, the combination of the cues does not increase and may even decrease the detectability of an edge. When bees land at an edge the orientation of their body axis is strongly affected by the visual cues defining this edge. Model simulations were performed to test whether the experimental findings can be explained on the basis of a single edge detection mechanism sensitive to both types of visual cues. In the model, the information from both types of cues is sensed by two fields of movement detectors that receive their input signals from two adjacent patches in the visual field. The output of all detectors subserving either patch is pooled by integrating cells. The signals of the two integrating cells subserving the two adjacent patches are compared at a subtraction stage. The resulting signal is then rectified and forms the output signal of the model. The model simulations closely resemble the experimental results, thus providing evidence that edge detection by the bee could be mediated by a single mechanism.

Animals↗

Vascular air embolism: location, frequency, and cause on electron-beam CT studies of the chest.

PURPOSE: To determine the frequency and location of venous air emboli that occur with the use of electron-beam computed tomographic (CT) studies of the chest. MATERIALS AND METHODS: Findings from 677 patients who underwent chest electron-beam CT with intravenous administration of contrast material were reviewed. Unenhanced CT studies were performed in 127 (18.8%) of these patients while the intravenous cannula was in place but before injection of contrast material. RESULTS: Air emboli were observed on CT studies in 79 (11.7%) of 677 patients. Emboli were small (up to three air bubbles less than 1 cm in diameter) in 70 (10.3%) patients and were moderate (more than three air bubbles or bubbles 1-2 cm in diameter) in nine (1.3%) patients. Air emboli were located in the main pulmonary artery (n = 54 [8.0%]), superior vena cava (n = 12 [1.8%]), right ventricle (n = 10 [1.5%]), subclavian or brachiocephalic vein (n = 6 [0.9%]), and right atrium (n = 5 [0.7%]). Seven patients (1.0%) had emboli in more than one location. Air emboli were depicted on unenhanced CT scans of seven (5.5%) of 127 patients. No association was found between the frequency of air embolism and injection flow, injection site, or amount or type of contrast agent. CONCLUSION: Intravenous administration of contrast material may cause small to moderate-sized air emboli. Knowledge of the common locations of emboli can help radiologists distinguish them from image artifacts or paravasal air collections.

Brachiocephalic Veins↗

[Functional studies of swallowing with electron beam computerized tomography].

Functional evaluation of swallowing disorders requires rapid imaging modalities. Videofluorography and cinematography are the gold standard, but they have their limitations: no transverse plane imaging is achieved and structural resolution for exact topographic analysis is limited. Three cases preselected by videofluorography were studied to evaluate whether electron beam tomography (EBT) permits more detailed dynamic imaging of swallowing disorders focusing on the mesonasopharyngeal segment, the hypopharynx and the upper esophageal sphincter (UES). Immediately after videofluorographic examination of the oropharyngeal deglutition, EBT is performed. The patient is in a supine position and while the patient swallows a 20 ml bolus of water or diluted iodine containing contrast agent, a sequence of 20 images per level is scanned. The levels, which are determined by using the scout view, are oriented parallel to the hard palate either at the level of the hard palate to image the mesonasopharyngeal segment or just above the hyoid bone to focus on the hypopharynx or at the location of the UES. The scan technique is a single-slice cinemode with a slice thickness of 3 mm (exposure time 100 ms, interscan delay 16 ms, 130 kV, 620 mA). The following structural interactions that we have so far been unable to image can be clearly demonstrated with EBT: (1) during normal swallowing, the mesonasopharyngeal segment is completely and symmetrically closed by the soft palate and Passavant's cushion; (2) lateral hypopharyngeal pouches can be located more precisely; and (3) disorders of the UES can be differentiated into functional or morphologically caused disorders (e.g., goiter or cervical osetophytes). Videofluorography and cinematography are still the gold standard in functional evaluation of swallowing disorders. However, EBT permits dynamic imaging of pharyngeal deglutition in a preselected transverse plane and can give useful additional information concerning functional anatomical changes in the pharynx during swallowing. Further clinical evaluation is needed.

Adult↗