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Biomedical subjects

R Kaufmann

Publications and source records attributed to R Kaufmann.

At least 325 records · Page 18Linked to original sources

Solubilized DNA-dependent RNA polymerase from human placenta: A Mn(2+)-dependent enzyme.

The 100-fold purified RNA polymerase activity from human placenta is completely dependent upon added DNA. The enzyme is most active at 3 mM Mn(2+) in the presence of 100 mM (NH(4))(2)SO(4). Denatured DNA is a better template than native DNA. alpha-Amanitin completely inhibits the incorporation of 3H-UMP, while rifampicin has no influence upon the enzymatic activity.

Journal Article↗