Biomedical subjects

R Kamimura

Publications and source records attributed to R Kamimura.

58 records · Page 4Linked to original sources

PubMedDate not supplied

Technique for procurement of heart, liver and kidneys from a single donor for transplantation.

The purpose of this report is to present a safe, simple technique for procurement of the heart, liver, and kidneys from a single donor with no warm ischemic time. Twenty mongrel dogs were divided into 5 groups of 3 recipients and 1 donor each. Liver and kidney mobilization was performed when the core temperature of the liver reached 27 degrees C, a result of ice slush in the abdominal cavity. As the core temperature of the liver reached 20-22 degrees C, the aorta and inferior vena cava were clamped just above the diaphragm. After excision of the liver and kidneys, another team harvested the heart as it continued to beat. The heart and liver were transplanted orthotopically after simple preservation for 12 and 6 hours, respectively. The kidney was transplanted heterotopically after simple 12-hour preservation. The maximum survival time was 7 hours for heart transplantation and 16 days for liver transplantation. Kidney homogenate ATP levels decreased to 19% of baseline after preservation and were restored to 68% one hour after reperfusion. Our method is simple and safe for harvesting the heart, liver, an kidneys for transplantation and may offer a definite possibility for clinical application.

Animals↗

PubMedDate not supplied

Simultaneous retrieval of the heart and liver from a single donor: an evaluation through preservation and transplantation.

The simple, safe, and feasible procurement technique for the heart and liver with no warm ischemic time is reported. Fifteen mongrel dogs were used to form two recipients and one donor combination in each experiment. A midline incision is extended from the suprasternal notch to the pubis, and a catheter is advanced into the aortic root by means of the brachiocephalic artery for monitoring systemic arterial pressure and later for coronary vascular washout with a cold cardioplegic solution. Liver mobilization is carried out first when the core temperature of the liver reaches 27 degrees C, obtained with ice slush in the abdominal cavity. As the core temperature of the liver reaches 20 degrees to 22 degrees C, the aorta and inferior vena cava are clamped just above the diaphragm. After excision of the liver a second team harvests the heart while it continues to beat. The heart and liver were transplanted orthotopically after simple preservation into a cold solution for 12 and 6 hours, respectively. The maximum survival time was 7 hours in heart transplantation and 16 days in liver transplantation. Our method is a simple, safe, feasible technique for acquiring the heart and liver or other visceral organs for transplantation and may have broad clinical application.

Adenosine Triphosphate↗

PubMedDate not supplied

Small giant cell carcinoma of the lung diagnosed preoperatively by transthoracic aspiration cytology. A case report.

Giant cell carcinoma (GCC) of the lung is considered an aggressive form of lung cancer. Most patients with GCC have large tumors with widespread metastases on hospital admission. We describe a small GCC in the lower lobe of the right lung. Preoperative aspiration cytology showed numerous bizarre, giant epithelial cells, highly suggestive of GCC. The resected tumor measured 1.2 x 1.0 x 0.7 cm in diameter, with an ill-defined margin. Histologically the tumor was composed of pleomorphic mononucleate or multinucleate giant cells without adenocarcinoma or squamous cell differentiation. The tumor cells were loosely organized and noncohesive or free in the alveolar space. The histology was interpreted as GCC of the lung. The clinicopathologic features of giant cell carcinoma are discussed.

Biopsy, Needle↗

PubMedDate not supplied

Effects of invert soap with 0.05% sodium hydroxide on infectious bursal disease virus.

The effects of invert soaps with sodium hydroxide on infectious bursal disease virus (IBDV) were studied. Didecyldimethylammonium chloride was most effective, followed by alkylbenzyldimethylammonium chloride and [mono-bis(tri-methylammonium-methylene chloride)]-alkyl (C9-15) toluene. Dilutions without NaOH had little effect on virus titer. Didecyldimethylammonium chloride was further tested for its effects on IBDV by varying temperature, concentration of invert soap, and pH of the dilution. The effect of the invert soap was strong at 40 C, moderate at room temperature, and weak at 4 C. The concentration of invert soap influenced its efficacy at room temperature but not at 4 C. At pH values below 12.9, the invert soap showed decreased efficacy.

Animals↗