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Biomedical subjects

R Kale

Publications and source records attributed to R Kale.

24 records · Page 2Linked to original sources
PubMed1983

No liquid holding recovery for chromosomal aberrations or sister-chromatid exchanges in irradiated G1 human lymphocytes.

We have tested G0 phase human peripheral lymphocytes for liquid holding recovery (LHR) mediated decreases in X-ray-induced chromosomal aberration yields and increases in sister-chromatid exchange (SCE) levels such as have been demonstrated for confluency-inhibited mouse cells in culture (Nagasawa and Little, 1981). No influence on either aberration yields or SCE levels was demonstrated. However, an effect at least superficially similar to the LHR effect was seen for rings and dicentrics, but not for deletions or SCE in lymphocytes in transition between G0 and G1 following PHA stimulation.

Chromosome Aberrations↗
PubMed1983

Cell and virus sensitivity studies with recombinant human alpha interferons.

The cell sensitivity of recombinant human alpha interferons (rIFN-alpha) of greater than 95% purity (A,D and the hybrid A/D) and crude nature (B and F) was studied in human (WISH, HeLa, AG1732), bovine (MDBK, BT), monkey (Vero), mouse (L), rabbit (RK-13), and hamster (BHK-21) cells. Based on an activity of 100% in WISH cells, the other cells responded to rIFN-alpha A as follows: AG1732 (90%), HeLa (94%), and MDBK and BT cells (170-190%). Rabbit, mouse, and hamster cells had a relative sensitivity of less than 1%. rIFN-alpha B and F were essentially equivalent to rIFN-alpha A in terms of cell sensitivity, but MDBK and BT cells were about 20 times more sensitive to rIFN-alpha D than were WISH cells and rIFN-alpha D was 1/5 to 1/10 as active on L cells as on WISH cells. The activity of the hybrid IFN A/D on bovine, mouse, and human cells was similar. The inhibitory dose50 (U/ml) of rIFN-alpha A, B, D, and F against virus infections in WISH cells were: vesicular stomatitis virus (1-4), rhinovirus types 1 and 42 (2-18), and herpes simplex virus (HSV) type 2 (45-70). Type 1 HSV, Semliki Forest (SFV) and encephalomyocarditis (EMC) viruses were tested against only rIFN-alpha A and D where SFV and EMC were the most sensitive to both IFNs (ID50-SFV, 0.2 U/ml, EMC, 1.2 U/ml) while 13 U/ml of rIFN-alpha A and D inhibited Type 1 HSV. The various rIFN-alpha s did not exhibit different antiviral spectra in vitro. When tested in mice rIFN-alpha A did not protect against infections with SFV, EMC, HSV, or influenza viruses. rIFN-alpha D and A/D protected mice infected with EMC, SFV, or HSV.

Animals↗
PubMedDate not supplied

JAMA editor sacked.

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American Medical Association↗
PubMedDate not supplied

Laparoscopic orchiopexy: is closure of the internal ring necessary?

BACKGROUND: Narrowing of the internal ring around the pulled through spermatic cord in cases of laparoscopic orchiopexy is the norm. AIM: To carry out a prospective study to see if closure of the internal ring is really necessary, hypothesis being that mobilization of the impalpable testis leaves a raw surface, which, coupled with the presence of the spermatic cord results in effective closure of the internal ring. METHODS AND MATERIALS: 51 patients with 65 impalpable testes underwent laparoscopic orchiopexy between July 1998 and June 2003. An impalpable testis was present in 21 cases on the left, 16 cases on the right and bilateral in 14 cases. Following complete mobilization required for orchiopexy, all testes with adequate length of spermatic cord were pulled down into the scrotum through the inguinal canal. No suture was applied to narrow the internal ring around the pulled through spermatic cord. Five testes were pulled down by an opening in the medial end of the inguinal canal due to inadequate length of the spermatic cord. In these cases the internal ring was closed by a suture. RESULTS: Follow up of all cases ranged between 1.5 years to 6.5 years. Not a single case has reported with recurrence of a hernia. CONCLUSION: The results suggest that narrowing of the internal ring around the pulled through spermatic cord may not be necessary.

Child↗