Prevention of deleterious oral habits.
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Child↗
Biomedical subjects
R K Roy
Publications and source records attributed to R K Roy.
Effects of deleterious oral habits on the dento-facial complex.
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Adolescent↗
Synthesis and processing of RNA by isolated human placental nuclei.
Conditions for isolating intact and active nuclei from human term pacenta and for studying their transcription products are described. The isolated nuclei can synthesize cell-free RNA for a prolonged period at 29 degree C in a medium containing 100 mM KCl and 5 mM MgCl2. Actinomycin D inhibited 92 per cent of RNA synthesis, whereas approximately 60 per cent of RNA synthesis was sensitive to alpha-amanitin. When nuclei were incubated at 29 degrees C for 1 h, about 27 per cent of the newly synthesized RNA was released into the medium outside the nucleus. Analysis of this released material by affinity chromatography on an oligo(dT)-cellulose column revealed that 2.4 per cent of the total released RNA was adsorbed at high salt concentration. Most of this fraction was eluted with a low-salt buffer at 45 degrees C and the remainder by 50 per cent formamide, conditions that are necessary for elution of poly(A)-containing mRNP particles from oligo(dT)-cellulose. These results show that placental nuclei incubated in vitro synthesize poly(A)-containing RNA, which is released as a protein-bound complex. This procedure allows exploration of changes in mRNA release during placental development.
Cell Fractionation↗
Improving the process quality using statistical design of experiments: a case study.
A technique known as Statistical design of experiments is a powerful technique for process characterization, optimization, and modeling. It has been widely accepted in manufacturing industry for improving product performance and reliability, process capability, and yield. This article illustrates the application of statistical design of experiments based on the Taguchi approach in a certain company that manufactures electromagnetic clutch coils. The objective of the study was to improve the quality of the existing process and thereby achieve heightened customer satisfaction for the product. An eight-trial experiment was conducted with the aim of reducing the number of rejects from the process. The expected savings per month was estimated to be over $11,500. The results of the study have provided a greater stimulus for the wider application of statistical design of experiments in other core processes within the company.
Analysis of Variance↗