Biomedical subjects

R K Naviaux

Publications and source records attributed to R K Naviaux.

24 records · Page 2Linked to original sources

PubMed1992

Retroviral vectors for persistent expression in vivo.

Retroviral vectors provide a safe and efficient method of introducing genes of therapeutic interest into dividing cells. The principle limitation of these vectors in the past has been poor gene expression in vivo. This problem has been overcome recently through the use of tissue-specific enhancers in commonly used retroviral vectors. In this review we discuss both the relevant biology and some of the practical applications of retroviral vectors in gene therapy.

Animals↗

PubMed1991

Human gene therapy.

Somatic cell gene therapy for the correction of many human genetic diseases is now technically possible. We review several methods of gene transfer that have been successfully used in animal studies, and discuss the promise and potential limitations of these methods in the treatment of human genetic diseases.

Cells, Cultured↗

PubMed1990

Construction and characterization of two infectious molecular clones of encephalomyocarditis virus.

We constructed and characterized two infectious molecular clones of encephalomyocarditis (EMC) virus. Both constructs, pDL and pDA, were assembled from five overlapping cDNA clones derived from the diabetogenic variant of EMC virus (EMC-D) and from two synthetic oligonucleotide cartridges. pDA contained a single point mutation at position 1720 within the "puff" region of capsid protein 1AB that was derived from the nondiabetogenic variant of EMC virus (EMC-B). This point mutation resulted in an amino acid substitution of arginine (EMC-B) for lysine (EMC-D). Our construction illustrates two novel findings: (i) that the problem of stably cloning long poly(C) tracts of EMC virus can be circumvented by the use of a shortened, synthetic, poly(dC-dG) oligonucleotide cartridge, and (ii) that a single point mutation in the puff region of the capsid protein 1AB leads to change in its electrophoretic mobility and to a change in the plaque size of recombinant virus.

Animals↗

PubMed1988

Comparison of the nucleotide sequences of diabetogenic and nondiabetogenic encephalomyocarditis virus.

The nucleotide sequences of a diabetogenic variant of encephalomyocarditis (EMC) virus (D variant, ifp- phenotype) and a nondiabetogenic variant of EMC virus (B variant, ifp+ phenotype) have been compared. These variants differ at eight sites. The poly(C) tract of EMC-B is three bases shorter than that of EMC-D. Of the seven sites at which nucleotide substitutions were confirmed using RNA templates, four resulted in amino acid changes in three different proteins, the leader peptide, 1B (VP2), and 1D (VP1). The biological significance of these differences can now be investigated.

Amino Acid Sequence↗

PubMed1986

Microimmunoassay permits determination of concentrations in immunohistochemistry controls.

A competition enzyme-linked immunosorbant assay (CELIA) can be used to determine the amount of antigen needed to immunoabsorb an antiserum. This is demonstrated using both polyclonal and monoclonal antibodies against human amylase. This method is of especial value when the tissue is particularly difficult to obtain, and especially valuable when CELIA has already been completed.

Amylases↗

PubMedDate not supplied

Micro competition enzyme linked immunosorbant assay for human apolipoprotein B.

A solid phase micro competition enzyme linked immunosorbant assay (microCELIA) was developed to measure apolipoprotein B (Apo B) in human plasma. The soluble Apo B competed with the solid phase antigen for antibody binding. After washing, alkaline phosphatase labeled goat anti-rabbit IgG antibody was added and the plates were washed and assayed. The minimal quantifiable concentration of Apo B was one mg per L. This enzyme immunoassay (EIA) yielded values which correlated with values of samples assayed in reference laboratories by radioimmunoassay (RIA) (r = 0.97) and by electroimmunoassay (r = 0.92). The electroimmunoassay overestimates the activity of hyperlipemic samples compared to microCELIA. The assay offers advantages over other existing techniques including short incubation time, high sensitivity, technical simplicity, elimination of radioisotopes, low cost, and use of a universal enzyme label.

Apolipoproteins B↗