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Biomedical subjects

R K Gupta

Publications and source records attributed to R K Gupta.

At least 685 records · Page 38Linked to original sources
PubMed1981

The long-term use of garlic in ischemic heart disease--an appraisal.

The hypocholesterolemic and fibrinolysis-enhancing properties of garlic were assessed in patients with ischemic heart disease (IHD) and in healthy control subjects. The peak of blood fibrinolytic activity (BFA) achieved at the 4th week of garlic therapy was not sustained despite its continuous use and returned to about the pre-garlic values at the 12th week. Garlic withdrawal did not cause any further change in BFA. Under the same conditions serum total cholesterol (STC) values did not show any significant change. Both of the foregoing features were observed in the IHD as well as in the control group. Garlic therapy for 12 weeks did not cause any appreciable changes in serum triglyceride, beta-lipoprotein, plasma fibrinogen levels or coagulation time in either IHD or control subjects. The evidence cited above does not appear to substantiate the prevalent popular belief in the efficacy of garlic in the management of IHD either as a hypocholesterolemic or as a fibrinolytic agent.

Adult↗
PubMed1981

Melanoma. Associated antigens in benign melanocytic disorders.

Three instances of depigmentation occurring in children with congenital large nevocytic nevi are reported. Serologic studies for tumour-associated antigens to melanoma were performed by indirect immunofluorescence and complement fixation on these patients and others with benign melanocytic disorders. Positive reactivity in several children was observed. The small numbers studied and lack of uniformity of other testing methods leave open the question of relevance of such reactivity to malignant conversion or protection in congenital nevi.

Adolescent↗
PubMed1981

Isoantigens A, B and H in primary liver malignancies.

Formalin-fixed tissues from 37 cases of primary liver malignancies were investigated by the technique of Specific Red Cell Adherence (SRCA) for studying the presence or absence of blood group isoantigens A, B and H. These tumours comprised 15 cases of hepatocellular carcinomas, 5 hepatoblastomas, and 17 cholangiocarcinomas. Results of the SRCA were consistently negative in all hepatocellular carcinomas and hepatoblastomas. Fifteen of 17 (88%) cholangiocarcinomas gave a positive SRCA, but no correlation could be obtained between the intensity of the reaction and their histological grading or metastatic potential. Reasons are given for the negative reactions in hepatocellular carcinomas and hepatoblastomas, an possible explanations are offered for the high rate of positivity in cholangiocarcinomas. It is concluded that the inherent properties of these 3 tumours limit the application of the SRCA procedure as a potential gauge of prognosis.

ABO Blood-Group System↗
PubMed1981

Thymus derived rosette forming cell counts and lymphocyte response to phytohaemagglutinin in animals affected with squamous cell carcinoma of horn.

A non-immune erythrocyte rosette test was used to examine the levels of thymus derived lymphocytes in the peripheral blood of 12 bovine horn cancer patients and an equal number of age-matched unaffected controls. The proportion of rosette forming cells (RFC) in horn cancer affected animals was found to be approximately half that of unaffected controls. Phytohaemagglutinin was used to examine lymphocyte transformation (LT), in vitro, of lymphocytes from the peripheral blood of six horn cancer affected animals as well as unaffected controls. Transformation values in affected animals were found to be much lower than in age-matched controls. Lower values of RFC as well as LT in the horn cancer affected animals compared with unaffected controls are suggestive of depression of cell mediated immunity in this condition.

Animals↗
PubMed1980

Hymenolepis nana: transfer of acquired immunity in mice through sensitized peritoneal exudate cells.

Sensitized peritoneal exudate cells from syngeneic donor Swiss albino mice infected with single and repeated doses of viable eggs of Hymenolepis nana produced a significant adoptive immunity in test mice when compared with animals which received non-sensitized (normal) cells. No significant difference was observed among the 2 recipient groups which received singly or repeatedly sensitized peritoneal exudate cells.

Animals↗
PubMed1980

Determination of incidence and partial characterization of tumor-associated antigens found in the urine of patients bearing solid tumors.

Urine samples from patients with solid tumors and from donors without malignant disease were concentrated and tested for the presence of tumor-associated antigens. In the complement-fixation assay using serum from a source autologous with the source of the urine, 87.4% of cancer patients were positive, while only 6.9% of control donors were positive. When serum from an allogenic source was used, 94.7% of cancer patients and 35.1% of control donors were positive. Absorption of a cancer patient's serum with autologous tumor cells removed antibody activity to autologous and allogeneic urine samples. Normal lymphocytes, skin, or muscle-cell suspensions were ineffective as absorbents. The excretion of antigen into urine is dependent upon the presence of tumor. Surgical removal of tumor resulted in cessation of antigen excretion. The urine remained antigen-negative as long as the patient remained disease-free. The antigenic activity was heat-stable and comprised molecules of greater than 1,000,000 daltons which could be dissociated into smaller molecular weight active fractions by treatment with 6 M urea.

Adolescent↗
PubMed1980

Presence of a carcinoma-associated antigen(s) in the spent chemically defined medium of a human colon carcinoma cell line.

The human colon carcinoma cell line HT-29 was adapted to grow in chemically defined medium (CDM). The spent CDM (S-CDM) was concentrated by Amicon filtration and the crude HT-29 S-CDM purified by 40% saturated (NH4)2 SO4 precipitation. The purified antigen was tested by a microcomplement fixation (MCF) assay against the sera of cancer patients of various histologic types and against the sera of normal donors. Fifteen of 20 (75%) colon cancer, 16/20 (80%) breast cancer sera, 14/19 (74%) lung cancer sera, and 13/20 (65%) miscellaneous carcinoma sera were positive in the MCF. By contrast, 2/21 (10%) melanoma sera, 7/20 (35%) sarcoma sera, and 2/19 (11%) normal sera were positive. These data suggest the presence of a carcinoma-associated antigen in the spent CDM of the HT-29 colon carcinoma cell line adapted to grow in CDM.

Antibodies, Neoplasm↗
PubMed1980

Production and characterization of xenogeneic antisera to tumor-associated antigen(s).

Tumor-associated antigen(s) from a specimen of human malignant melanoma and from spent culture medium of a melanoma cell line were extracted and purified. The incidence of antibody activity to these antigens, in sera from cancer patients with neoplasms of various histologic types, was higher (65--83%) than normal donors' (19--25%) by the complement fixation assay. These purified antigens were then used to raise antisera in rabbits and sheep. After absorption with various human normal tissues, these antisera reacted against melanoma, sarcoma, and carcinoma extracts, but not against human normal liver, skin, or muscle extracts. However, the antisera showed reactivity against a human fetal homogenate. Results indicate that the xenogeneic antisera contained antibodies to fetal antigens and to tumor-associated antigen(s). Xenogeneic antisera could be used to purify tumor-associated antigens and oncofetal antigen(s) from crude extracts by affinity chromatography.

Animals↗