Biomedical subjects
Publications and source records attributed to R K Archer.
Healthy Lac:P Wistar rats reached a plateau for red-cell parameters at about 18 weeks of age. For the next 9 months the values remained substantially constant. White-cell parameters varied in a more complex manner, and were largely unrelated to body-weight.
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Haematological determinations were carried out on 7 inbred strains of rats using a standardized procedure. Significant genetic variation was found for many of the parameters although none of the strains had values which could be termed pathological. Measurements also varied significantly from day to day. Factorial analysis of variance is shown to be a powerful method for the investigation of variation in haematological parameters.
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A sedimentation method for counting human blood platelets is proposed which uses a fluid of sp.gr. i.057 at an osmolarity of 320 milliosmols. The fluid is a mixture of Lymphoprep and Isoton II. After centrifugation at about 200 platelets are counted on an electronic particle counter.
In the search among biological particles for a standard for counting human platelets, a strain of Absidia corymbifera was found to have spores that resembled platelets. After fixing in formalin and autoclaving the spores had a similar mean cell volume to that of human platelets. A suspension of these killed spores was tested in 100 laboratories and gave consistent results as a standard for human platelet counting. The Absidia corymbifera standard can be used in electronic counting methods but not in sedimentation methods, as the spores will be removed by centrifugation.
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Based on morphologie studies on bone marrow, lymphnode and blood preparations the cells of equine blood and their developmental stages are described and illustrated with 32 coloured photographs.
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