Search PubMed⌕ Search

Biomedical subjects

R Jonsson

Publications and source records attributed to R Jonsson.

At least 163 records · Page 9Linked to original sources
PubMed1987

Expression of HLA-D-locus (DP, DQ, DR)-coded antigens, beta 2-microglobulin, and the interleukin 2 receptor in Sjögren's syndrome.

The expression of beta 2-microglobulin (beta 2m), class II major histocompatibility complex (MHC) antigens (HLA-DP, -DQ, -DR) and the interleukin 2 receptor (IL-2R) on resident and infiltrating cells in labial salivary glands of patients with Sjögren's syndrome (SS) was studied using an immunoperoxidase technique based on staining with monoclonal antibodies. The progression of the inflammatory process was accompanied by increasing numbers of immunocompetent cells as well as glandular epithelial cells expressing beta 2m and class II MHC antigens. Up to 60% of the infiltrating lymphocytes were DQ- and DR-positive while fewer cells stained for DP antigen. Glandular epithelium (acinar and ductal cells) stained for products of all three HLA-D subregions but with varying degrees of expression following the pattern DR greater than DP greater than DQ related to the severity of inflammation. A minor portion of the infiltrating lymphocytes expressed IL-2R. These findings emphasize the potential importance of epithelial expression of class II antigens in the local activation of T lymphocytes in salivary gland lesions of SS patients. They also indicate that differences may exist in this respect between T-cell reactions restricted by different class II MHC-encoded gene products.

Arthritis, Rheumatoid↗
PubMed1987

Immunohistochemical characterization of sialadenitis in NZB X NZW F1 mice.

The spontaneously developing sialadenitis in female autoimmune NZB X NZW F1 (NZB/W) mice has been studied with the help of immunohistochemistry and monoclonal antibodies to cell surface antigens. Semiquantitative assessment of stained cells within the infiltrates disclosed a progressive focal inflammation most pronounced in submandibular and parotid glands. The majority of cells expressed Ly-1 (all T cells) and L3T4 (T helper) phenotype, whereas only few Lyt-2 (cytotoxic/suppressor) expressing T cells were seen. A large proportion of the infiltrating cells stained for Ia antigens, which was also found on salivary gland ductal epithelium in the proximity of lymphoid infiltrates. The phenotypic pattern in sialadenitis of NZB/W mice thus closely resembles the pattern previously described for human Sjögren's syndrome (SS). Accordingly, immunomorphological analysis of the NZB/W sialadenitis may be useful in further studies of pathogenesis and therapy of both experimental and human SS.

Animals↗
PubMed1987

T cell subsets and expression of immunological activation markers in the arterial walls of patients with giant cell arteritis.

Immunohistochemical features of infiltrating mononuclear cells (MNC) and resident cells were studied in the temporal artery biopsy specimens of 13 patients with histological verified giant cell arteritis (GCA) and in six biopsy specimens from patients with GCA with negative histological findings. Eight temporal artery biopsy specimens from seven patients with unrelated diseases served as controls. In all patients with GCA proved by biopsy an infiltration of T lymphocytes in the arterial wall was observed, most being of the helper/inducer subset. No B lymphocytes, or very few, were seen. Lymphocytes in 10 out of the 13 positive biopsy specimens displayed staining for the class II major histocompatibility complex (MHC) antigen HLA-DR, whereas this was found in only two of eight controls. A minor number of the infiltrating T lymphocytes from seven out of 13 patients with GCA proved by biopsy stained for transferrin receptors, and in six out of the 13 cases they reacted with anti-interleukin 2 receptor antibody. In the arterial wall from all patients with histologically verified GCA we also found an increased number of macrophages, many of them expressing HLA-DR antigens and transferrin receptors. The immunohistochemical pattern of cell phenotypes found in the arterial wall of patients with GCA suggests that the infiltrating T cells are immunologically activated. This finding supports the hypothesis of a predominantly cellular immunological pathogenesis of giant cell arteritis.

Aged↗
PubMed1987

Sialadenitis in the MRL-l mouse: morphological and immunohistochemical characterization of resident and infiltrating cells.

The MRL-lpr/lpr (MRL/l) mouse spontaneously develops an autoimmune disease associated with various rheumatic manifestations. We have studied the histological features of sialadenitis and utilized an immunohistochemical staining technique on frozen tissue sections to analyse the cellular composition of the salivary glands at varying stages of disease. Semiquantitative assessment of the infiltration disclosed a focal inflammation which started at 2 months of age and was progressive until 5 months of age in submandibular glands. A high frequency of Ly-1 and L3T4-positive cells was observed at all stages of sialadenitis, whereas Lyt-2 positive cells were found at a lower frequency. Ia expression was seen on a large proportion of the infiltrating cells and also on ductal and glandular epithelial cells in the vicinity of the inflammatory lesions. The presence of Ia antigens on many inflammatory as well as resident cells and the high frequency of lymphocytes with 'helper' phenotype may indicate a perpetuated immunological activation within salivary glands.

Animals↗
PubMed1986

A demineralization procedure for immunohistopathological use. EDTA treatment preserves lymphoid cell surface antigens.

An evaluation of the usefulness of EDTA treatment for decalcification of murine bone tissue in order to preserve both morphological details and immunologically intact cell surface antigens has been performed. The ABC immunohistochemical staining technique employing monoclonal antibodies to subsets of T-lymphocytes, B-lymphocytes and to Ia antigens was used on frozen sections. Treatment of mouse hindlegs with EDTA for 14 days resulted in an efficient decalcification and good preservation of morphological details. When lymphoid tissues were handled in the same manner monoclonal antibodies, defining Ly 1, Ly 2, L3T4, MAS 034 and Ia molecules, were shown to retain their reactivity comparable to that of directly frozen tissues. In contrast, formic acid, the commonly used decalcification agent, destroyed most of the antigenic reactivity. We conclude that EDTA treatment of non-fixed, bone-containing tissues provides a suitable demineralization procedure in the immunohistochemical study of, e.g., arthritis and periodontitis.

Animals↗
PubMed1986

The salivary gland component of Sjögren's syndrome: an evaluation of diagnostic methods.

The diagnostic value of sialography, scintigraphy, sialometry, and labial salivary gland biopsy--as indicators of salivary gland dysfunction in Sjögren's syndrome (SS)--was evaluated in 41 patients suspected of having SS. In about 70% of the cases, each of the four examinations showed changes that could indicate a salivary gland component of SS. However, the disease specificity of sialographic and scintigraphic examinations was low. Although the specificity of the labial salivary gland biopsy examination is considered high, our study revealed some cases in which a focus score of 1 to 3 was not accompanied by abnormal changes in either sialometry, sialography, or scintigraphy. At a focus score of more than 3, a fair amount of agreement on the results of the various diagnostic procedures was found. Because labial salivary gland examination shows only minor salivary glands and sialography and scintigraphy include only major salivary glands, variance between different diagnostic procedures is expected. This indicates a potential diagnostic role for sialographic and scintigraphic examination when the labial salivary gland biopsy is insufficient.

Adult↗
PubMed1985

Alterations in fibronectin distribution in oral mucosal vesiculo-bullous lesions.

The distribution of fibronectin in human oral mucosal vesiculo-bullous diseases was studied by indirect immunofluorescence. Subepithelial (benign mucous membrane pemphigoid and erythema multiforme) as well as intraepithelial (pemphigus vulgaris and dyskeratosis follicularis) lesions were selected. A marked accumulation of fibronectin was generally found in the lamina propria and basement membrane region, except in dyskeratosis follicularis. Intraepithelial staining occurred in erythema multiforme and dyskeratosis follicularis. In pemphigus vulgaris intercellular staining was seen in relation to acantholysis. Both mucous membrane pemphigoid and erythema multiforme showed a linear accumulation corresponding to the intact basement membrane. However, only pemphigoid lesions disclosed fibronectin on the epithelial side of the vesicles.

Darier Disease↗
PubMed1984

Ultrastructural localization of fibronectin in duct cells of human minor salivary glands and its immunochemical detection in minor salivary gland secretion.

Fibronectin (Fn) was localized in duct cells by means of a light and electron microscopic immunohistochemical technique. The subcellular localization demonstrated that Fn is synthesized in these cells and thus not exclusively produced by cells with a mesodermal background. Fn concentration, as measured by radioimmunoassay, was higher in saliva from the minor salivary glands than in unstimulated whole saliva, whereas Fn was undetectable in stimulated parotid saliva. Fn is thus a conspicuous component in unstimulated resting saliva. Fn may be an important factor, negative or positive, for the integrity of the oral hard and soft tissues because it has the capability to bind and agglutinate microorganisms.

Fibronectins↗
PubMed1984

Assessment of Langerhans' cells in oral lichen planus using monoclonal antibodies.

In order to demonstrate Langerhans' cells in epithelium of oral lichen planus, monoclonal antibodies were used as immunological markers in combination with immunohistochemistry. By the use of anti-Ia antibodies the Langerhans' cells were shown to express an increased number of Ia-like antigens in comparison to the amounts found in healthy oral mucosa. The subepithelial infiltrate of mononuclear cells expressed identical Ia-like antigens on their surfaces. With anti-T6 antibodies as immunological markers, the number of Langerhans' cells was found to be virtually identical in diseased and healthy epithelium. Treatment of oral lichen planus with tretinoin resulted in a decrease of epithelial Ia-like antigens compared with the number found in untreated lesions. However, treatment with tretinoin did not alter the frequency of Langerhans' cell marked with anti-T6 antibodies. The present data demonstrate an increased amount of Ia-like antigens per number of T6-positive Langerhans' cells in diseased oral mucosa compared to healthy conditions. The increased expression of Ia-like antigens on Langerhans' cells and the contemporary finding of Ia-like antigens on the subepithelial T-cells support the opinion that the pathogenesis of oral lichen planus is mainly a cell-mediated type of immunological reaction.

Antibodies, Monoclonal↗
PubMed1984

Oral mucosal lesions in systemic lupus erythematosus--a clinical, histopathological and immunopathological study.

The oral mucosa was investigated in 51 patients with systemic lupus erythematosus (SLE). Lesions were seen in 23 patients (45%). Most lesions were of discoid type or erythemas; only 3 were seen in 23 patients (45%). Most lesions were of discoid type or erythemas; only 3 were ulcerations. The hard palate, buccal mucosa and vermilion border were the most frequent sites of involvement. The overall prevalence was not related to disease activity, but discoid lesions and ulcerations were only seen in patients with active disease. Histopathological investigation showed liquefactive degeneration in 6 biopsies of 10 lesions. Immunofluorescence examination was positive in all cases.

Adult↗
PubMed1983

Temporomandibular joint involvement in systemic lupus erythematosus.

Temporomandibular joint involvement was studied in 37 systemic lupus erythematosus patients not selected with regard to this involvement. One-third had current complaints and two-thirds had a history of severe symptoms. Objective findings indicating systemic lupus erythematosus arthritis were locking or dislocation, tenderness to palpation, and pain on movement of the mandible. Such changes were found in 8 patients (22%) but in none of the healthy controls. Radiographic changes of the condyles, including flattenings, erosions, osteophytes, and sclerosis, were seen in 11 patients (30%), 4 of whom had deformity of other joints as well.

Adult↗
PubMed1983

Changes in bone mineral content of the axial skeleton in relation to aging and the menopause. Results from a longitudinal population study of women in Gothenburg, Sweden.

Patterns of bone loss in the axial skeleton have been studied in a sample of Swedish women participating in a longitudinal population study which was started in 1968. In 1976, the mineral content of the lumbar spine (predominantly trabecular bone) was measured in vivo in 130 women by dual photon absorptiometry. Premenopausal or recently postmenopausal women were compared with women of identical age who had been postmenopausal for a long time. The first group was found to have significantly higher values of bone mineral content. Five years later, in 1981, the same women were re-examined with identical techniques. A slight decrease in bone mineral content with age was found in postmenopausal women. The findings were mostly in agreement with those of the first cross-sectional study, with bigger differences in bone mineral content between women of different menstrual status than between women of different age. In addition, the lower values in women with early menopause compared to those with late menopause remained in spite of increasing age.

Aged↗