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Biomedical subjects

R Jenkins

Publications and source records attributed to R Jenkins.

At least 181 records · Page 10Linked to original sources

Society of Gastrointestinal Radiologists Roscoe E. Miller Award. Monitoring hepatic cryosurgery with sonography.

The intraoperative sonographic appearance of frozen hepatic tumors and frozen normal liver was correlated with the histologic appearance of pathologic specimens from six patients. Frozen tissue was visualized as a hyperechoic rim with posterior acoustic shadowing. Normal liver that was frozen and then thawed appeared hypoechoic when compared with normal unfrozen liver. These findings allowed visualization of the extent of freezing in relation to tumor margins. Pathologically, frozen tumors showed definite evidence of necrosis. Normal liver tissue was extremely sensitive to cold and was completely necrotic after freezing. The sonographic appearance of frozen and thawed liver correlated precisely with the gross morphologic and histologic changes seen in the cryolesion. It appears that intraoperative sonography allows accurate monitoring of hepatic cryosurgery.

Carcinoma↗

Implementation of an i.v. morphine infusion program in a community hospital.

Pharmacy departments play a role in helping to design and implement a pain management program in community hospitals. The history, documentation of need, and benefits of the program are presented. Protocol guidelines for the intravenous infusion of morphine are outlined, and specific job functions of the nurse and pharmacist members of the pain management team are defined. In addition, the results of a pain management study are reported, which describe the efficacy of the treatment protocol in 22 patients.

Hospital Bed Capacity, under 100↗

Use of intraoperative blood salvage during orthotopic liver transplantation.

Human liver transplantation is a developing surgical technique that requires a large volume of blood support. There are no published results of the use of intraoperative blood salvage in liver transplantation. We used automated intraoperative blood salvage during 13 initial consecutive human liver transplants. The procedures required a median of 32 units of packed red blood cell support, of which an average of 45% was supplied by intraoperative salvage. The percent of the total blood use provided by intraoperative salvage increased with increasing total blood needs. Intraoperative blood salvage applied to liver transplantation resulted in a net savings of blood and hospital costs of approximately $1,000 per procedure.

Adult↗

Immunohistochemical demonstration of S-100 protein in salivary gland neoplasms.

A peroxidase-antiperoxidase technique for S-100 protein has been applied to 68 salivary glands. These included 17 pleomorphic adenomas, seven adenoid cystic carcinomas, 23 adenolymphomas and a number of other neoplasms. In addition, five specimens of myoepithelial sialadenitis ('benign lymphoepithelial lesion') and five normal parotid glands were included. Consistent results were obtained, myoepithelial cells and cells in myxoid and chondroid areas in pleomorphic adenomas staining intensely. In adenoid cystic carcinoma, on the other hand, there was no staining. The adenolymphomas possessed intensely S-100 protein-positive cells in the interfollicular lymphoid areas; these were probably interdigitating reticulum cells. In addition, branching structures, probably corresponding to Langerhans' cells, were observed in the epithelium of adenolymphomas.

Adenolymphoma↗

Sex differences in minor psychiatric morbidity: a survey of a homogeneous population.

Epidemiological methods of psychiatric assessment were used to examine whether there is a sex difference in the constitutional vulnerability of the male and female phenotype to minor psychiatric morbidity. In order to minimise the effect of environment and of sex roles and stereotypes as far as possible, a sample of relatively homogeneous employed men and women are studied. No sex difference in prevalence of minor psychiatric morbidity or its outcome was found in this population of men and women of similar age, education, occupation and social environment. However, women did report significantly more somatic symptoms of psychogenic origin.

Adult↗

A study of the classification of mental ill-health in general practice.

An outline is provided of an experimental study of the classification of mental ill-health presented to general practitioners (GPs). Videotaped real-life consultations were assessed by 27 GPs for the purpose. A high degree of inter-observer variation was recorded. Some of the implications of the findings are discussed.

Family Practice↗

Sex differences in minor psychiatric morbidity.

This monograph is concerned with some epidemiological observations of minor psychiatric morbidity. Differences in rates of specific disorders have historically formed a crucial part of epidemiological enquiry. Sex differences in the prevalence of minor affective disorders have been demonstrated in studies of populations in treatment and in community populations, and have been variously ascribed to constitutional or environmental factors. A review of the literature exposes the methodological problems of measurement and study design, and the conflicting nature of the findings. Efforts have recently concentrated on establishing the social factors important in the aetiology of minor affective disorder without adequate exclusion of constitutional factors. The importance of life events, chronic social stress and inadequate social supports in the aetiology of minor affective disorder has been demonstrated, but the variance explained by such factors is small, reinforcing the view that constitutional factors of some kind are likely to be of importance. The evidence for a genetic contribution to minor affective disorder is tenuous, but there is circumstantial evidence that changes in gonadal hormones are linked to mood changes in women. Until further evidence is available this must remain a powerful possibility in the genesis of sex differences in minor affective disorder. This investigation used epidemiological methods to assess whether there is a sex difference in the constitutional vulnerability of the male and female phenotype to minor psychiatric morbidity. In order to minimize the effects of environment and of sex roles and stereotypes as far as possible, a sample of relatively homogeneous employed men and women (drawn from a population of executive officers in the Home Office) was studied. The data obtained from this population of men and women of similar age, education, occupation and social environment were compatible with the null hypothesis that there is no sex difference in the prevalence of minor psychiatric morbidity, or its outcome in such a homogeneous group. However, women did report significantly more somatic symptoms than men. The second hypothesis, that there is a substantial sex difference in the self-perception of illness, illness behaviour and sickness absence in individuals with minor psychiatric morbidity among a homogeneous group of men and women of similar age, education and occupation, and subject to similar levels of social stress and support, received only partial support from the findings of the study.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗

The effect of non-steroidal anti-inflammatory therapy on plasma neuropeptide concentrations in patients with osteoarthritis.

Plasma levels of the endogenous opioid peptides beta-endorphin and [Met]enkephalin were estimated in 10 osteoarthritic patients during treatment with a sustained-release indomethacin preparation (Osmosin). Significant diurnal variation of beta-endorphin levels was evident both on and off treatment (p less than 0.05, respectively), but the therapy was nevertheless accompanied by decreased morning levels of this peptide; no such changes were recorded for [Met)enkephalin. Whilst the treatment was associated with a reduction of pain, particularly in the evening (p less than 0.02), no correlation was evident between pain and beta-endorphin levels, nor between the patients' perception of pain relief and changes in beta-endorphin levels. The results suggest either that prostaglandins may be involved in the synthesis/release of beta-endorphin, or that the alleviation of stress may in turn reduce the need for continued beta-endorphin production in these patients.

Aged↗

Immunohistochemical localization of cathepsin G in human tissues.

An antiserum to human cathepsin G has been raised in sheep and its reactivity with human tissues has been tested. The indirect immunoperoxidase staining sequence was employed and was applied to routinely processed paraffin sections. Mature granulocytes, especially those of the neutrophil variety, were intensely and consistently stained. Activity was not observed in other cell or tissue types. Many of the cells of acute and chronic myeloid leukemia were strongly stained, in contrast to those of acute lymphoblastic or chronic lymphocytic leukemias. The results of the technique are compared with those described with staining for muramidase (lysozyme), alpha 1-antitrypsin, leukocyte elastase, and naphthol-AS-D-chloroacetate esterase, and with certain monoclonal antisera directed against granulocyte determinants.

Cathepsin G↗

An immunohistochemical study of branchial cysts.

Twenty five specimens of branchial cyst from the same number of patients have been examined. On staining with haematoxylin and eosin a consistent finding was that the mural lymphoid follicles were always aligned with their mantle zones towards the luminal epithelium. With conventional staining lymphatic sinuses were noted in 17 of the specimens, but with immunohistochemical staining these structures were apparent in 23 cysts. Their frequent occurrence in branchial cysts supports the theory that these lesions are derived from epithelial inclusions in lymph nodes. Immunohistochemical techniques for a range of other markers, using polyclonal and monoclonal antisera, showed a distribution of lymphoid and non-lymphoid tissue elements, as seen in lymph nodes and, for example, palatine tonsils. The lining luminal epithelium also shared many features in common with the crypt epithelium of tonsils.

Adult↗

Cells which contain S-100 protein in Hodgkin's disease: a quantitative study.

Paraffin sections from a series of 50 lymph nodes affected by Hodgkin's disease were examined by means of the unlabelled primary antibody peroxidase-antiperoxidase method to detect those cells which contained S-100 protein. In addition, 15 lymph nodes showing reactive follicular hyperplasia were studied. A simple enumeration procedure (eyepiece graticule) was used to count the number of such cells in 20 standard 25 X microscope fields. In the specimens of nodular sclerosing Hodgkin's disease many cells positive for S-100 protein were present, in contrast to the other Rye subtypes, which showed a relative paucity. By comparison, the lymph nodes showing reactive follicular hyperplasia contained a similar number of cells containing S-100 to those seen in the nodular sclerosing lymph nodes affected by Hodgkin's disease.

Cell Count↗

Minor psychiatric morbidity in employed young men and women and its contribution to sickness absence.

An epidemiological survey of male and female executive officers in the Civil Service showed a prevalence of 33% of minor psychiatric disorders. Follow up one year later found that only half were better. The presence of minor psychiatric disorder was associated with increased rates of sickness absence, particularly certified absence. Such a high prevalence, associated as it is with handicap to the individual and consequences for the working environment, indicates the need for early medical detection and treatment.

Absenteeism↗

Minor psychiatric morbidity and labour turnover.

The relation of minor psychiatric morbidity with labour turnover is examined, using data from a study of young, predominantly middle class, white collar men and women. The results suggest that the presence of psychiatric symptomatology is at least as important as occupational attitudes in identifying individuals who would subsequently leave the organisation.

Absenteeism↗

Glutamic dehydrogenase from rat heart mitochondria. I. Purification and physical properties including molecular weight determination.

Glutamic dehydrogenase extracted with tris buffer from fresh freeze-thawed rat heart mitochondria was purified by ammonium sulphate fractionation, affinity chromatography on GTP agarose, hydroxyapatite chromatography and concentration using a molecular sieve. The final specific activity is 80 units/mg protein. Thin gel SDS electrophoresis of the purified enzyme preparation after reduction with dithiothreitol shows a major band with a molecular weight of 38 000 Daltons. Two minor bands are also present. Sucrose density gradient centrifugation reveals a molecular weight of 230 000 Daltons for unreduced mitochondrial GDH activity. By gel filtration rat heart mitochondrial glutamic dehydrogenase has a major peak at 230 000 Daltons, a minor peak at 300 000 Daltons and some larger molecular weight species. Rat liver mitochondrial glutamic dehydrogenase has a minor peak at 230 000, a major peak at 300 000 and some larger molecular weight species. The rat liver mitochondrial glutamic dehydrogenase predominance at 300 000 is unchanged by incubation, extraction and purification with rat heart mitochondria. The purified GDH is stable frozen at -10 degrees C in tris-HCl buffer with EDTA. It loses activity at 4 degrees C especially when stored in 0.2 M phosphate buffer. It also loses activity when dialyzed for 24 h. This loss of activity is not completely prevented by adding nucleotides to the buffer (AMP or ADP) but is decreased by their presence.

Animals↗

Glutamic dehydrogenase from rat heart mitochondria. II. Kinetic characteristics.

Glutamic dehydrogenase purified from rat heart mitochondria has been characterized with regard to its substrate kinetics and the influence of nucleotides and potassium phosphate on its kinetic properties. The enzyme had characteristics similar to liver mitochondrial glutamic dehydrogenase. These included several double reciprocal plots which were biphasic, indicating homotropic interaction; inhibition by GTP, which was overcome by ADP and phosphate; and activity with both NAD(H) and NADP(H). There were a number of significant differences however, in the specific kinetic properties of heart mitochondrial glutamic dehydrogenase. The Vmax of reductive amination was four-fold greater with NADH than with NADPH. The maximum rate of oxidative deamination was ten-fold greater with NAD compared to NADP. The differences also included: saturating levels of NADH and NADPH were stimulatory rather than inhibitory; ammonia was stimulatory at millimolar levels; NADP and alpha-ketoglutarate were both inhibitory at saturating levels; and ADP increased reductive amination 30% at lower levels of NADH but inhibited at higher (stimulatory) levels of NADH.

Adenosine Diphosphate↗