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Biomedical subjects

R Janisch

Publications and source records attributed to R Janisch.

At least 37 records · Page 2Linked to original sources
PubMed1985

Ultrastructure of eggs of Ascaris lumbricoides Linnaeus, 1758. I. Egg-shells.

Under the light microscope the chitin-protein layer of egg-shells in ascarids appears to be a regular, hyaline and nonstructural layer of 1.5 to 2.00 microns in thickness. The outer uterine layer is usually removed during the preparation. The lipid (ascaroside) layer covers the inner surface of the chitinous layer and seems to be irregularly undulated and regularly thick over the whole surface, with the thickness up to 1 micron. In electron micrographs the fibrous structure of the lipid layer is not evident as a rule. This is probably due to washing the lipids away from this layer during the dehydration of deeper layers of egg-shells that are imperfectly fixed with glutaraldehyde. A very low permeability of the egg-shells is typical of geohelminth eggs. The layer lipid shows a distinct lamellate structure only after a prolonged fixation with osmium at higher temperature. This is supported by the studies using the method of freeze-fracturing.

Animals↗
PubMed1981

Membrane structures of Acholeplasma laidlawii and its virus.

The main types of ultrastructures found in the freeze-fracture faces of Acholeplasma laidlawii S 2 and its virus MV-Lg-L 172 were (1) particles 7-19 nm in diameter, mostly located in the convex cytoplasmic fracture faces. (2) small bulges or aggregates, 13-25 nm in diameter, which occupied only limited areas of both inner and outer fracture faces of some mycoplasmas, (3) numerous tiny grains and/or spikes 2-6 nm in diameter, protruding from a finely structured background, especially in the outer concave mycoplasmal fracture faces, and (4) linear structures, most probably fibrils and thicker filaments, both in the fracture faces and around mycoplasmas and viruses and connected with them. There was a high degree of structural similarity between mycoplasmal and viral membranes: no obvious significant difference was found.

Acholeplasma laidlawii↗
PubMed1981

Mycoplasma-virus contacts, budding and scars remaining in the membranes of Acholeplasma laidlawii and its virus.

Various stages of virus and mycoplasma budding indicated that both virus and, most probably some mycoplasma progeny developed by budding. Besides this alternative, binary fission was the mode of mycoplasma reproduction. Mycoplasma-virus and mycoplasma-mycoplasma connections by stems were observed. Circular scars, 40-80 nm in diameter, often in groups, were left in the membrane of mycoplasmas by the budding bodies. cytoplasmic structures seen in cross-fraction are presented. a relatively small number of globular virus-like bodies, not identical with MV-Lg-L 172, were observed budding from mycoplasma cells in the non-infected host culture.

Acholeplasma laidlawii↗