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Biomedical subjects

R J Long

Publications and source records attributed to R J Long.

17 recordsLinked to original sources

Effect of strategic feed supplementation on productive and reproductive performance in yak cows.

A total of 2230 yak cows (5-13 years of age) in two populations with different milking systems were investigated. One population had a system of milking once a day (MOD), and the other population twice a day (MTD). The average milk yield of MOD cows was 0.7 +/- 0.2 kg/day within a milking period of 109 +/- 9 days. This compared with an average of 1.24 +/- 0.3 kg/day in 127 +/- 6 days in MTD yaks (p < 0.01). The cows showed a calving rate of 71% under the MOD system and 51.4% under the MTD system. Three farms with a total of 104 MTD cows between six and 12 years of age were used to provide three different feeding groups. The groups were fed with, or without oat hay or highland barley straw in amounts of 1-1.5 kg/head/day from December to April. The three farms were designated as Farm I, Farm II, and Farm III. Farm I had 41 cows with body weight of 230 +/- 67 kg each for grazing with no supplement (GNS). Farm II had 30 animals with body weight of 216 +/- 28 kg each for grazing + oat hay (GOH). Farm III had 33 animals body weight of 221 +/- 34 kg each for grazing + highland barely straw (GBS). The calving rates of the cows in GOH and GBS were 23 and 19% higher, respectively, than GNS cows (p < 0.01), and the highest rate reached 76.7% in GOH. The live weight loss of the cows in GNS was considerably higher (p < 0.01) than in the two other groups. Ten GOH cows and 12 GNS cows were used to collect milk samples for measuring the progesterone concentration using RIA kits provided by IAEA/FAO: Milk was sampled every five days from calving until 90 days postpartum. In the unsupplemented group, milk progesterone (P4) levels suggested that cows had started cyclic ovarian activity by 40 days postpartum, whereas only 25% had been observed in estrus. In the supplemented group, 80% of cows had started cyclic ovarian activity by the same time and 70% had been seen in estrus. Two types of cyclic activity in terms of progesterone changed were found. With Type I (normal), 50 and 80% of cows from GNS and GOH, respectively, had cyclic changes of P4 in milk at 40 days postpartum. With Type II, the P4 levels in the milk remained 0.89 ng/ml until 90 days postpartum. A total of 46 grazing cows between five and 13 years of age (body weight 214 +/- 68 kg) was used to collect blood samples to measure concentrations of nutrient of metabolites at two weeks pre-calving and at two weeks, two months and four months postcalving, respectively. The concentrations of nutrient metabolites [albumin, globulin, urea, beta-hydroxybutyrate (BHB) and inorganic phosphorus] suggested general underfeeding of energy and protein in the winter/late pregnancy period with some recovery in lactation. Energy constraints appeared again as the summer progressed. No dietary phosphate deficiency was found. BHB and albumin testing on serum yaks could be a useful tool to identify poor nutritional status during the winter and so illustrate the need for supplementation.

Animal Feed↗

Occupational injuries: a study of health care workers at a northwestern health science center and teaching hospital.

1. This retrospective study examined the magnitude of the problem of work related health hazards affecting professional and non-professional employees of a state university health science center and hospital. 2. From the 1,513 injury episodes that occurred among the 9,668 employees during the 32 month study period, it was determined that the highest risk categories for both department and job were nursing, housekeeping, food service, and laboratory technicians. 3. Injury rate for females (11.2 per 100 person years) was more than twice that of males (5.1). Injury rates declined from 11.6 per 100 person years at ages 25 to 39 to 3.8 at ages over 60. Puncture wounds (32.7%), mostly by needlesticks, were the most frequently reported injury type, followed by strains and sprains (17.2%), lacerations (12.5%), and contusions (12.1%).

Academic Medical Centers↗

Inability of awake patients to correctly locate a cough stimulus.

To determine whether patients can accurately identify the lung in which a cough reflex originates, a unilateral cough stimulus was administered to 67 patients during fiberoptic bronchoscopy. Although the stimulus produced a vigorous cough reflex in all subjects, 51 of 67 individuals were unable to identify the side stimulated. These results suggest that impulses from endobronchial irritant receptors are not perceived cortically as originating within a particular hemithorax.

Bronchoscopy↗

Endotoxin protects selenium-deficient rats from hyperoxia.

Rats treated with low doses of bacterial endotoxin have been shown to be protected from oxygen poisoning under normobaric conditions. Induction of lung activity of the antioxidant enzymes glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) has been reported to occur with endotoxin administration. GSH-Px is a selenoenzyme and selenium-deficient rats have decreased lung GSH-Px activity and enhanced lung toxicity during a hyperoxic exposure. To determine whether bacterial endotoxin administration can provide protection for animals with decreased antioxidant defenses, selenium-deficient and control rats received daily intraperitoneal injections of 250 micrograms/kg bacterial endotoxin or phosphate-buffered saline (PBS) during normobaric exposure to greater than 95% O2. Both groups of animals were protected from hyperoxia by bacterial endotoxin administration despite the extremely low lung GSH-Px activity in the selenium-deficient rats. GSH-Px, SOD, or CAT activities were not induced in the selenium-deficient rats by 48 hr (the time when the selenium-deficient rats treated with PBS began to die). In the selenium-deficient rat, mechanisms other than enzyme induction appear to be providing early protection from hyperoxia.

Animals↗