Bovine leucosis virus: antibody in cattle in New South Wales.
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Biomedical subjects
Publications and source records attributed to R J Hoare.
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A mastitis control program based on teat dipping and dry cow therapy was evaluated in 35 herds over a 3 year period. The incidence of subclinical mastitis as detected by the Rapid Mastitis Test (RMT) fell from 34% to 12% quarters positive, a reduction of 65%. Clinical mastitis was reduced from 37 clinical cases per 100 cows in the first 3 month period to 12 clinical cases per 100 cows in the last 3 month period, a reduction of 68%. Variations in response to the program in the reduction of subclinical and clinical mastitis are discussed and the results compared with similar trials conducted overseas. The Modified Whiteside Test was used on bulk milk samples from the control herds and these results were significantly correlated with the prevalence of subclinical mastitis assessed by RMT individual quarters.
Bacteriological examinations were made on quarter samples from cows in 35 herds over a 3 year period to monitor the response in a mastitis control program. Initially, Staphylococcus aureus predominated in 32 of the herds and the mean herd prevalence was 26%. The control measures halved this rate but there was considerable variation in response between herds. The decline occurred rapidly and there was a significant reduction (P less than 0.01) by 3 months. Streptococcus agalactiae predominated in 3 herds and the overall infection rate was 4.9%. Control measures eliminated the infection completely from most herds but reinfection occurred in 2 herds. The greatest decline occurred in the first 6 months and was significant (P less than 0.05). The measures had little effect upon Str. uberis and Str. dysgalactiae which remained fairly consistently at low levels. Initially, strains of Staph. aureus resistant to penicillin were dominant in most herds. In a minority of herds strains resistant to streptomycin predominated and in these herds there was a concurrent resistance to penicillin. These patterns did not change greatly over the control period. Resistance by Str. agalactiae to streptomycin occurred in most herds at the start of the program.
In conjunction with a Coulter Counter, somatic cells in milk were sized by electronic analysis. Quarter milk from cows with mastitis had a cell volume peak with a modal cell volume of 102 mu3 while milk from healthy quarters had no peak. Bulk milks with a peak had higher cell counts than milks where there was no peak. Dimensions of peak from bulk milks were the same as from quarter milks. Modal cell volume and cell count varied after milks were fixed with varying concentrations and types of fixative. The concentration of fixative recommended by the International Dairy Federation was sufficient, but only marginally so, and it should be increased.
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Iron uptake and release by ferritin molecules of different iron contents show similar profiles. These are discussed in relation to the structure of the ferritin molecule. Two models of iron uptake and release are considered. One involves iron oxidation-reduction sites on the protein. The other allows direct interaction of reagents with the iron-core crystallites. It is concluded that the second model accounts better for the experimental results presented now and in previous publications.
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