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Biomedical subjects

R Itoh

Publications and source records attributed to R Itoh.

At least 163 records · Page 9Linked to original sources
PubMed1993

[Immunohistochemical determination of endometrial progesterone receptor (PR) content after intrauterine infusion of danazol in rabbits].

Danazol, an isoxazole derivative of 17 alpha-ethinyl-testosterone, has various effects on the female reproductive system. To examine its anti-estrogenic effects through the receptor system and clarify its direct effect on the endometrium in vivo, we applied danazol jelly into the rabbit uterine cavity. Recent studies performed in our laboratory using the ligand-binding assay have shown that danazol administered directly into the uterine cavity is absorbed by the endometrial tissue and exerts its anti-estrogenic effects possibly through progesterone receptors (PR) in the cells. In the present study we examined the variations in endometrial PR content during 7 days after the intrauterine administration of danazol into the rabbit uterine cavity. Immature female white rabbits were used. They were given daily subcutaneous injections of estradiol-17 beta (E2) for 3 days after which their abdomens were opened, and danazol gel was infused into the lumen of the right horn and HPC-jelly was infused into the lumen of the left horn, as the control. Thereafter and until they were killed, the same daily dose of E2 was injected. They were killed 6h, 24h, 3 days, 5 days or 7 days after intrauterine infusion of danazol and PRs were studied immunohistochemically using a specific antireceptor monoclonal antibody (PR-AT 4.14). Six hours after danazol infusion, the PR staining in the glandular epithelium and part of the stroma surrounding the epithelium had declined. On day 3, PR staining declined to its lowest level in the glandular epithelium and stroma. On Day 5, PR staining had been restored in the glandular epithelium but stayed low in the stroma.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1992

A comparative study on tissue distribution and metabolic adaptation of IMP-GMP 5'-nucleotidase.

1. Activity of a cytoplasmic 5'-nucleotidase which preferentially hydrolyzes IMP and GMP (IMP-GMP 5'-nucleotidase) was determined by a specific immunochemical method in two species of birds and two species of mammals. 2. The activity was markedly high in avian liver, and it increased two-fold in response to a high protein diet in chicken liver. 3. In mammals, the activity was high in testis and spleen. In the rat, the activities in liver, kidney and heart extracts increased by about 30% in response to the high protein diet, while they increased three-fold in regenerating liver. 4. Low activities were detected in skeletal muscles and in erythrocytes of all the species studied.

5'-Nucleotidase↗
PubMed1992

Pulmonary metastatic nodules: CT-pathologic correlation.

To elucidate the characteristics of pulmonary metastatic nodules on high-resolution computed tomographic (HRCT) scans, a correlative computed tomographic (CT)-pathologic study was performed with five human lungs after autopsy. The relationship of metastatic nodules to pulmonary vessels was studied with HRCT scans, radiographs of the specimen, and stereomicroscopic study in 264 nodules 0.6-9.0 mm in diameter. On radiographs and stereomicroscopic images, 190 small nodules (less than 3 mm in diameter) were in contact with the pulmonary lobule on the central bronchovascular bundles (n = 33 [17.4%]), located between the central bronchovascular bundle and the perilobular structure (n = 127 [66.8%]), or attached to perilobular structures (n = 30 [15.8%]). On HRCT scans, 21 small nodules (11.1%) were located on the central bronchovascular bundle; 130 small nodules (68.4%), between the central bronchovascular bundle and the perilobular structure; and 39 small nodules (20.5%), on the perilobular structure. On radiographs and stereomicroscopic images, 43 of 74 large nodules (greater than 3 mm in diameter) (58%) compressed both bronchovascular bundles and perilobular structures. The central bronchovascular bundle was invaded in only 13 large nodules (18%).

Humans↗
PubMed1992

Multivariate analysis of dietary habits in 931 elderly Japanese males: smoking, food frequency and food preference.

To define the influence of smoking on dietary patterns and food preference in elderly Japanese, a mail survey was conducted on about 3000 subjects aged 65 years and older. A multivariate analysis of dietary frequency data regarding 15 food items on 931 male respondents suggested that frequency of intake of plant food, including vegetables and fruits, was lower in smokers than in nonsmokers. Another multivariate analysis of food preference data regarding 18 food items on the same subjects suggested that smoking decreased the desire for plant food.

Aged↗
PubMed1992

Familial spinal xanthomatosis with sitosterolemia.

A family with multiple spinal xanthomas and sitosterolemia is described. A 48-year-old woman presented with paraplegia due to multiple intradural extramedullary tumors. The patient also showed marked tendon xanthomas and analysis of sterol composition in both plasma and the xanthoma established the diagnosis of the rare inherited metabolic disease, sitosterolemia and xanthomatosis. Two other siblings in the family presented with marked tendon xanthomas and coronary atherosclerosis, but did not show any neurological signs or symptoms. Magnetic resonance imaging (MRI) study revealed multiple intradural extramedullary tumors in spinal canals of the proband and her sister, but not in the other affected sibling (brother). This is the first report of familial occurrence of multiple extramedullary spinal tumors due to the inherited metabolic abnormality.

Cholesterol↗
PubMed1992

Scanning electron microscopic study on the lingual papillae in the Manchurian chipmunk, Tamias sibiricus asiaticus.

The superfacial structures of the tongues in the Manchurian Chipmunk, Tamias sibiricus asiaticus, were observed by scanning electron microscope. The tongues were long, tapering, narrow and thick with a long apical free portion and a small lingual prominence in the posterior half. In this animal, three circumvallate papillae were present in an inverted triangle, a minority of conical papillae on the pharyngeal part and parallel large conical papillae on the lateral border. The fungiform papillae were scanty on the dorsal surface. These characters suggested this animal was more primitive than the others in rodents.

Animals↗
PubMed1992

[Estimation of medroxyprogesterone acetate against a human endometrial tumor constituted from the established Ishikawa cancer cells by a subrenal capsule assay].

In order to establish a sensitivity test system for the evaluation of anti-cancer hormonal agents, we tried a long-term subrenal capsule (SRC) assay, using nude mice with a transplanted solid tumor of endometrial cancer cells (Ishikawa's line). Unlike DNA-affecting agents, anti-cancer hormonal agents exert cytostatic effects rather than cytocidal effects, and their evaluation in a short period is considered to be inaccurate. Our test system is somewhat difficult in terms of technique, but it is useful since it can (1) evaluate the agents in the relatively short period of 28 days and (2) compare the cytostatic anti-tumor efficacy of two or more agents under the same conditions. The rate of successful tumor transplantation in nude mice in our system is very high, i.e., more than 90%. Although there are some points which need improvement, our system is considered to be useful as an assay system for the development of anti-cancer hormonal agents and other similar chemotherapeutic agents for cancers. When medroxyprogesterone acetate (MPA) was evaluated by means of this system, the administration period, as well as the dosage, was found to be important.

Animals↗
PubMed1992

[Effect of high-dose medroxyprogesterone acetate on coagulative and fibrinolytic factors in patients with gynecological cancers].

Medroxyprogesterone acetate (MPA), which is widely used clinically as an anticancer steroid preparation, is a very useful drug that seldom causes severe side effects such as bone marrow suppression, and can be dispensed at the outpatient clinic for an oral administration at home to the advantage of QOL. Recently however, there have been several reports suggesting its relationship with thrombosis. We measured t-PA, protein C, factor X, AT III, TAT, plasminogen, PIC, fibrinogen, and D-dimer in 11 patients with gynecologic malignancies who are treated with MPA (600 mg/day) and 11 controls. Then we examined the effects of the drug on blood coagulation and fibrinolytic activities. No changes in these parameters clearly suggested thrombogenesis in either group at this measurement or during the observation period (17 months at the maximum). The present study found no remarkable abnormalities in the blood coagulation and fibrinolytic activities. Thus, to avoid the use of MPA to patients at risk is considered to be the most important precaution for prevention of thrombosis.

Antithrombin III↗
PubMed1991

The bialaphos biosynthetic genes of Streptomyces viridochromogenes: cloning, heterospecific expression, and comparison with the genes of Streptomyces hygroscopicus.

The bialaphos resistance gene, bar, was used as a selectable marker to isolate the bialaphos production genes (bap) from the Streptomyces viridochromogenes genome. The S. viridochromogenes bar gene was cloned on overlapping restriction fragments using pIJ680 and pIJ702 in the bialaphos-sensitive host, S. lividans. Although the restriction endonuclease cleavage map of these fragments was not similar to the bap cluster of S. hygroscopicus, the presence and location of bar and four other bap genes as well as a gene required for the transcriptional activation of the cluster (brpA) was demonstrated by heterologous cloning experiments using a series of previously characterized bialaphos-nonproducing S. hygroscopicus mutants. Since recombination-deficient mutants of streptomycetes have not been isolated, restored function provided by cloned homologous DNA results from both recombination (marker rescue) and complementation in trans. In contrast to our previously reported homologous cloning experiments where we were able to define the position of mutant alleles by recombination, in these heterologous cloning experiments we observed little if any recombination between plasmid-cloned genes and the chromosome. As a result, this approach allowed us to define the location and orientation of functional genes using a genetic complementation test. The organization of the clustered S. viridochromogenes bap genes was indistinguishable from the corresponding S. hygroscopicus mutant alleles. The fact that the S. viridochromogenes transcriptional regulatory gene, brpA, functioned in S. hygroscopicus implied that some transcriptional regulatory signals may also be interchangeable. In these two Streptomyces species, which have considerable nucleotide sequence divergence, the complex biochemical and genetic organization of the bialaphos biosynthetic pathway is conserved.

Anti-Bacterial Agents↗
PubMed1991

Nutritional status of vitamin A in a healthy elderly population in Japan.

In this study nutritional status of vitamin A (VA) in a healthy elderly population in Japan was examined. Healthy elderly volunteers with mean age of about 67 at the start of the study were recruited. Analysis of serum VA concentration was carried out four times in two consecutive years. No deficient subject was detected from the serum VA concentration. Only small percentages were assessed to be in a low or marginal VA status. Consequently, the nutritional status of VA in the elderly Japanese seemed to be adequate judging from the serum VA concentration. Contrary to that, mean values of daily dietary intake of VA in both females and males were almost acceptable but the respective values varied over a wide range with no correlation with the serum VA concentrations. Accordingly, serum VA concentration provided a more definitive evaluation to assess VA nutriture than its dietary intake.

Aged↗
PubMed1991

The interrelation of urinary calcium and sodium intake in healthy elderly Japanese.

To evaluate whether habitual excess sodium intake is significant as a risk factor for calcium loss in elderly Japanese, a relationship between calcium excretion and sodium excretion was studied in 39 male and 44 female Japanese, aged 60 and over. They are healthy, free-living and consuming a self-selected diet. A significant positive correlation between urinary calcium excretion and sodium excretion per 24-hour was observed both in males (r = .415, p less than 0.01) and in females (r = .330, p less than 0.05). The relationship was still significant even after adjustment for sex, age, body mass index, alkaline phosphatase levels and protein as well as calcium and phosphorus intake. It was estimated that the increase in urinary calcium excretion was about 1.0 mmol/day per 100 mmol increase in urinary sodium excretion.

Aged↗
PubMed1990

Serum ascorbic acid and HDL cholesterol in a healthy elderly Japanese population.

The relationships between HDL cholesterol and serum ascorbic acid were examined in 79 male and 96 female healthy elderly Japanese, aged 60 and over. A significant positive correlation between serum HDL cholesterol and ascorbic acid was observed both in males (r = .243, p less than 0.05) and in females (r = .376, p less than 0.01). The results of multiple regression analyses indicated that serum ascorbic acid is a predictor of serum HDL cholesterol accounting for about 5% and 11% of its total variation in male and female subjects, respectively. The effect of age, BMI, status of physical activity, alcohol ingestion and cigarettes consumption, serum triglycerides and total cholesterol were taken into account as potentially confounding variables.

Aged↗