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Biomedical subjects

R Ismail

Publications and source records attributed to R Ismail.

At least 55 records · Page 3Linked to original sources
PubMed1989

Ferritin mRNA is found on bound as well as on free polyribosomes in rat heart.

Free and endoplasmic reticulum-bound polyribosomes from rat heart were examined for their ferritin mRNA content. A procedure for separation and purification of the two ribosome populations that produced good yields of homogeneous mono- and polyribosomes with no contaminating ultrastructures and gave distinctive sedimentation profiles in 15-50% sucrose gradients was developed. 14C-labeled free and bound polyribosomes added to heart preparations indicated that only 3% of free and 5.5% of bound polyribosomes cross-contaminated the bound and free fractions, respectively. RNA from both polyribosome populations hybridized with [32P]cDNA for rat ferritin. The extent of hybridization with mRNA from endoplasmic reticulum (ER)-derived polyribosomes was much greater than what could be accounted for by cross-contamination with free polyribosomes. This indicates that heart ferritin is synthesized not only on free polyribosomes for internal use in iron storage but also on ER-bound polyribosomes, where it may be destined for secretion into the plasma.

Animals↗
PubMed1989

Does angiotensin regulate systemic conversion of prorenin to renin?

Within 12 hours of binephrectomy in rats, plasma prorenin rises about 250% above the pre-operative baseline and remains above-normal for at least 48 hours, indicating an extrarenal source of prorenin. Concurrently, active renin disappears, implying the loss of a renal "convertase" mechanism for prorenin activation. Such "convertase" activity was detected in incubates of renal cortical slices. To test the effect of angiotensin (Ang) on prorenin/"convertase" regulation, we infused Ang I (100 ng/kg/min) intraperitoneally for 24 hours and obtained evidence of "convertase" inhibition, as happened also following Ang II (2uM) addition to incubated cortical slices. Thus, the release and/or activity of "convertase" appears to be regulated by Ang in-vivo and in-vitro, suggesting that Ang controls not only direct renal renin release but also the secretion and/or activity of a renal "convertase" capable of producing additional renin from circulating prorenin pools.

Angiotensin I↗
PubMed1988

One type of gamma-turn, rather than the other gives rise to chain-reversal in proteins.

Gamma-turns may be defined by a hydrogen bond between the carbonyl group of one amino acid residue and the amino group of the acid two residues ahead in the sequence. They occur as two types, inverse gamma-turns and classic gamma-turns (classic gamma-turns are usually called just gamma-turns but we prefer to add the adjective classic to distinguish them from the word gamma-turn, referring collectively to both). Of the two, classic gamma-turns are less common and are considered by all authors to be extreme rarities in proteins. However, we find that a number do occur in a sample of proteins of known three-dimensional structure. One occurs at the edge of the second hypervariable region of the light chain in some immunoglobulins. All classic gamma-turns except one are associated with a reversal in the main chain direction. In most cases, the turn lies at the loop end of a beta-hairpin. By contrast, inverse gamma-turns, although giving rise to a kink in the chain, rarely occur within beta-hairpins and are seldom situated at a position of reversal, by 180 degrees, in chain direction.

Amino Acids↗
PubMedDate not supplied

Gonococcal vulvovaginitis among female children in Malaysia.

In this retrospective review of 16 children with vulvovaginitis due to Neisseria gonorrhoeae, the authors attempt to estimate the incidence of this infection and to ascertain the routes of transmission. From January 1977 to December 1982, 422 cases of gonococcal vulvovaginitis in women of all age groups were encountered at the University Hospital, Kuala Lumpur, Malaysia. Of these, 406 cases (96.2%) occurred in adults, and 16 cases (3.8%) occurred in children younger than 12 years of age. Penicillinase-producing N. gonorrhoeae (PPNG) strains were isolated from five (31%) of the 16 girls; all five cases were subsequently treated with cefuroxime and probenecid. The rest responded to intramuscular procaine penicillin. Although all patients appeared to be cured, only ten of 11 (two with PPNG) had test-of-cure cultures performed after treatment. Although interviewing the parents revealed no history of sexual contact in these children, nine of the girls were linked to culture-positive adult(s).

Child↗
PubMedDate not supplied

Infections due to beta-lactamase-producing Neisseria gonorrhoeae at the University Hospital Medical Center, Kuala Lumpur, Malaysia.

The incidence of infections due to beta-lactamase-producing Neisseria gonorrhoeae is increasing in many parts of the world. An epidemiologic survey of infections caused by beta-lactamase-producing strains of N. gonorrhoeae at the University Hospital, Kuala Lumpur, from February 1977 to December 1985 (106 months) showed that the incidence rose from 4.8% (two cases) in 1977 to 49.4% (39 cases) by the end of 1985. The highest incidence of gonococcal infections was found to be in the group aged 20-39 years; the male-to-female ratio was 1.55:1. The mean inhibitory concentrations of benzylpenicillin were 0.12 microgram/ml for non-beta-lactamase-producing strains and 16 micrograms/ml for isolates of N. gonorrhoeae that produce beta-lactamase.

Adolescent↗
PubMedDate not supplied

Prevalence of obsessive compulsive symptoms (OCS) in a sample of Egyptian adolescents.

The aim of this work was to determine the prevalence of OCS among a community sample of Egyptian students. The sample was selected using a multistage stratified random sample of students from El Abasseya educational area in Cairo. The tools used in this study included the General Health Questionnaire for screening of psychiatric morbidity and the Arabic Obsessive Scale for obsessive traits. The Yale Brown Obsessive Compulsive Scale was used to determine the profile of OCS and the ICD-10 research criteria for diagnosis of OCD among OCS positive subjects. The prevalence of psychiatric morbidity among the total sample was 51.7%, whilst that of obsessive traits was 26.2% and that of obsessive compulsive symptoms was 43.1%. OCS were more prevalent among the younger students, among female students and first born subjects. Aggressive, contamination and religious obsessions and cleaning compulsions were the commonest among the sample; 19.6% of subjects with OCS fulfilled ICD-10 criteria for OCD.

Adolescent↗
PubMedDate not supplied

[Tuberculosis of the transverse colon. Apropos of 2 cases].

The authors report two cases of primitive tuberculosis of the transverse colon. Its rare occurrence and the importance of endoscopy are pointed out. A therapeutic test is often justified. Surgery is needed in case of acute complications or stenosis, and when a malignant lesion cannot be ruled out.

Adult↗