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Biomedical subjects

R I Levy

Publications and source records attributed to R I Levy.

At least 163 records · Page 9Linked to original sources
PubMed1971

On thelipoprotein abnormality in type 3 hyperlipoproteinemia.

Two lipoprotein species were isolated by starch block electrophoresis from the very low density lipoproteins (VLDL) (S(f) 20-400) of patients with type III hyperlipoproteinemia. One of these, alpha(2)-VLDL, had a content of lipid and protein and physical characteristics similar to VLDL from normal subjects or patients with other forms of hyperglyceridemia. The other species, beta-VLDL, contained more cholesterol and less triglyceride in relation to the protein, than normal VLDL. Only the apoprotein of low density lipoprotein was immunochemically detectable in the beta-VLDL; the proteins in the alpha(2)-VLDL reacted with antisera specific for low density lipoprotein and high density lipoprotein. The electrophoretic mobility of beta-VLDL was similar to that of low density lipoprotein and significantly less than that of alpha(2)-VLDL. Isolated beta-VLDL had a lesser mean flotation rate than alpha(2)-VLDL, but both alpha(2)- and beta-VLDL were found throughout the S(f) 20-400 flotation range.The relative quantities of alpha(2)- and beta-VLDL could be varied by changing the diet or by heparin administration. Most of the VLDL from type III patients on a high carbohydrate diet was in the alpha(2)-VLDL form. During fasting, alpha(2)-VLDL fell and beta-VLDL increased becoming the predominant species of VLDL. Heparin-induced acceleration of triglyceride clearance also increased beta-VLDL and decreased alpha(2)-VLDL. These findings suggest a precursor-product relationship between the alpha(2)- and beta-forms of VLDL.

Adult↗
PubMed1970

Identity of very low density lipoprotein apoproteins of plasma and liver Golgi apparatus.

In the rat, very low density lipoproteins isolated from hepatocyte Golgi apparatus, liver perfusates, and whole plasma appear identical in many respects. With specific immunochemical techniques and polyacrylamide-gel electrophoresis it can be demonstrated that the very low density lipoproteins from all three sources contain the same major lipoprotein apoproteins.

Animals↗