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Biomedical subjects

R Hunt

Publications and source records attributed to R Hunt.

At least 109 records · Page 6Linked to original sources
PubMed1977

Preclinical toxicological study of phosphonoacetic acid: determination in blood by selected ion monitoring.

Confirmed observations of the inhibition of oncogenic viruses by phosphonoacetic acid led to preclinical trials in animal model systems. Circulating phosphonoacetic acid was detected in the blood of mouse, rabbit and monkey after oral or subcutaneous administration of the drug. Phosphonoacetic acid is quantified in blood, after removing proteins and lipids, as the trimethylsilylated derivative, by monitoring the intensity of the protonated molecular ion and also that of phosphonopropionic acid (internal standard) using chemical ionization mass spectrometry combined with gas chromatography. The detection limit is 20 ng ml-1 when 0.2 ml serum is analyzed. A dosage of 230 mg kg-1 day-1 by continuous infusion is proposed for therapeutic trials in monkeys. This dose is well tolerated and results in slowly increasing blood levels of phosphonoacetic acid which reach a maximum of approximately 50 microgram ml-1 in a four day infusion, and decrease to 2 microgram ml-1 24 h after termination.

Animals↗
PubMed1977

Importance of the spleen for the immuno-inhibitory aciton of linoleic acid in mice.

Certain immuno-inhibitory effects of a polyunsaturated fatty acid, linoleic acid (C18:2), no longer occur after splenectomy of young adult CBA mice. This observation suggest that the spleen is a major intermediary in the action of C18:2 on the lymphoreticular system. Pathways of this action remain to be elucidated. Possibilities include C18:2-stimulated suppressor cell generation in the spleen, or excess biosynthesis of immuno-inhibitory prostaglandins by splenic macrophages.

Animals↗
PubMed1976

Hyperphenylalaninaemia and experimental allergic encephalomyelitis.

Previous suggestions to the effect that a reduced proportion of long chain and unsaturated fatty acids in the CNS as induced by experimental hyperphenylalaninaemia in young rats may alter the biochemical reactivity and stability of the myelin have been examined using experimental allergic encephalomyelitis (EAE). Lewis rats treated chronically with phenylalanine during development showed a higher susceptibility to EAE and a more severe course of the disease than their medium-treated litter mates. The possible implications of this observation for EAE as an experimental model of multiple sclerosis (MS) is discussed briefly in the light of the decreased levels of unsaturated fatty acids found in the CNS of MS patients.

Animals↗
PubMed1976

Influence of polyunsaturated fatty acids on survival of skin allografts and tumor incidence in mice.

Subcutaneous or oral administration of the polyunsaturated fatty acid linoleic acid prolongs survival of skin allografts in mice. Mice fed on a diet deficient in polyunsaturated fatty acids show a relative immunopotentiation, as indicated by accelerated skin allograft rejection and decreased incidence and rate of development of methylcholanthrene-induced tumors. These observations support the hypothesis that polyunsaturated fatty acids take part in immunoregulatory mechanisms.

Animals↗
PubMed1976

The significance of embryonic reexpression in cancer.

Irradiated syngeneic mouse testicular cells and the first-trimester human fetal tissue cells protect mice partially against tumors induced by methylcholanthrene or against a syngeneic transplantable mouse tumor, Meth A, respectively. For future research it is suggested that experiments should have a therapeutic rather than a prophylactic design and that the induction of tolerance to fetal antigens could be a highly informative procedure.

Animals↗
PubMed1975

The diagnosis of asbestosis.

Lung biopsy in 16 patients with abnormal lung function and who had been exposed to asbestos revealed asbestosis in 4 with considerable radiological evidence of the disease, in of 4 with minimal radiological changes and in none of 8 with completely clear radiographs. Thus biopsy for the diagnosis of this condition should be avoided if the chest radiograph shows no evidence of it.

Adult↗