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Biomedical subjects

R Hope

Publications and source records attributed to R Hope.

33 records · Page 2Linked to original sources
PubMed1985

Cloning and chromosomal location of the alpha- and beta-globin genes from a marsupial.

The cDNA sequences encoding the adult alpha- and beta-globin polypeptides of a marsupial, the native cat Dasyurus viverrinus, have been cloned and their nucleotide sequences have been determined. Using these cDNA clones we have determined the chromosomal location of the native cat alpha- and beta-globin genes by in situ hybridization to fixed metaphase chromosomes and by hybridization to DNA extracted from chromosomes purified by a fluorescence-activated cell sorter. Using two independent methods of gene assignment we have shown the alpha- and beta-globin-like gene sequences to be asyntenic in the native cat, the alpha-globin-like sequences being on chromosome 2 and the beta-globin-like sequences being on chromosome 4.

Animals↗
PubMed1976

Penicillin-induced haemolytic anaemia associated with microangiopathy.

The development of a penicillin-induced haemolytic anaemia was studied in a patient with microangiopathy following an average daily dose of five million units of penicillin G (total dose 46 million units). It has been proposed that the combination of the benzylpenicilloyl hapten with exposed or altered erythrocyte antigens, induces an autoimmune response. In this particular case, it is suggested that erythrocyte membrane damage has predisposed to immune drug-mediated red cell damage.

Adult↗
PubMed1975

Type V hyperlipoproteinaemia: a family study.

Family screening for hyperlipoproteinaemia was undertaken when a patient presented with myocardial infarction in association with Type V hyperlipoproteinaemia. The clinical features of this unusual form of hyperlipoproteinaemia are discussed together with its frequency in the general population and in relatives of affected patients.

Adolescent↗
PubMedDate not supplied

A monoclonal antibody, R1, and a polyclonal serum, S10, recognize the same molecules: a novel use of DNA transfectants.

The monoclonal antibody R1 defines the product of the MIC5 locus which has been localized to the long arm of the human X chromosome in the same region as the fragile site FRAXA. DNA transfectants, selected by R1 on the fluorescence-activated cell sorter (FACS), have been used to demonstrate that a previously described polyclonal antiserum, S10 (Buck & Bodmer, 1976), recognizes the same cell surface molecule as R1. Immunoprecipitation from metabolically and surface-labelled cells has shown that this molecule is a Mr 200 K phosphoprotein which is synthesized as a Mr 180 K precursor and subsequently modified to a Mr 195 K extracellular form. A Mr 150 K molecule is coprecipitated with the Mr 200 K phosphoprotein, but is only detected in lysates of surface-labelled cells, raising the possibility that the 200 K molecule is a cell surface receptor and the 150 K molecule a ligand.

Animals↗