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Biomedical subjects

R Holland

Publications and source records attributed to R Holland.

At least 163 records · Page 9Linked to original sources
PubMed1989

Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast.

Using immunohistochemistry, the distribution patterns of basement membrane components type VII collagen (monoclonal antibody LH7.2), type IV collagen, and laminin were investigated in normal and malignant human breast tissue and compared with that of keratin 14 (monoclonal antibody LL002), which is expressed only by the basal (myoepithelial) cells in the secretory epithelia of the mammary gland. In normal breast tissue as well as in intraductal carcinomas, a more or less continuous basement membrane was observed at the epithelial stromal interface. Unlike laminin and type IV collagen, type VII collagen was not detected in the basement membrane of blood vessels. The keratin 14 antibody stained the basal cell layer of normal ducts and ducts with in situ cancer. In 85% of the invasive carcinomas no basement membrane or basal cells were detected. In 13 cases, however, laminin, type IV collagen, and/or type VII collagen were detected around tumor nests and individual tumor cells. Five of these tumors also showed a positive reaction with the keratin 14 antibody. In five cases keratin 14 expression was found without detectable basement membrane components. It is concluded that 18 of 103 invasive ductal breast carcinomas examined in this study exhibit a basal cell phenotype as determined from the expression of keratin and the deposition of basement membrane components.

Basement Membrane↗
PubMed1988

Identifying psychiatric suicides for research purposes.

Unusual accidental deaths recorded for former public psychiatric patients during a 3-year period in Missouri were compared with data previously published on suicide and undetermined deaths. Demographic and diagnostic data suggested that undetermined deaths resemble suicides, while the remaining unusual accidents do not. For purposes of estimating incidence or prevalence, it is recommended that "undetermined deaths" might well be combined with suicides, while the remaining accidents should not.

Accidents↗
PubMed1988

Pyoderma gangrenosum in acute myeloid leukaemia during immunosuppression.

We describe a patient who developed pyoderma gangrenosum during the remission phase of acute myeloid leukaemia whilst receiving maintenance therapy with methotrexate and 6-mercaptopurine. The spontaneous resolution of these skin lesions following discontinuation of chemotherapy suggests that these drugs may be of major significance in the aetiology of pyoderma gangrenosum. Nevertheless, 27 months later, a relapse of the leukaemia followed. Although pyoderma gangrenosum occurred during clinical remission, we cannot rule out a synergism of leukaemia and chemotherapy in its pathogenesis.

Adult↗
PubMed1988

Neonatal jaundice in cystic fibrosis: a conservative approach is not always justified.

Biliary obstruction due to cystic fibrosis is considered to be reversible and conservative management is recommended. We present two cases of cystic fibrosis with anatomic lesions of the extrahepatic bile ducts, necessitating operative correction. In the management of prolonged neonatal jaundice in cystic fibrosis, a conservative approach is frequently successful, but in some cases surgical intervention may be mandatory.

Bile Ducts↗
PubMed1987

Malignant fibrous histiocytoma of the breast with axillary lymph node involvement.

This case history describes a 70-year-old female patient with a malignant fibrous histiocytoma (MFH) of the breast with secondary involvement of the skin and axillary lymph node metastases. We think that in the case of a MFH of the breast a radical or modified radical mastectomy should be pursued instead of a simple mastectomy.

Aged↗
PubMed1987

The predictive value of positive test results in screening for breast cancer by mammography in the Nijmegen programme.

After 10 years of screening for breast cancer by mammography in Nijmegen, the predictive value of positive screening results (PV+) was evaluated. The percentage of women with breast cancer in the group of referred women (PV+) for women under age 50 was 16-26%, regardless of the number of screening examinations they had. The percentage of women with breast cancer in the group of women who were biopsied was 25-40%, regardless of the number of examinations. For women aged 50 and over the predictive value was 34-57% and 58-90% respectively. It was further evaluated whether characteristics such as age, Quetelet index, parity, and Wolfe-classification could be used to increase the PV+ in women who were identified as positive by mammography. A logistic regression model analysis showed that true-positive and false-positive cases differ significantly only in terms of age and breast complaints. Although the model had a good fit, it could not be used to distinguish false-positive from true-positive test results.

Age Factors↗
PubMed1987

Oxygen saturation during transfer from operating room to recovery after anaesthesia.

Transcutaneous oxygen saturation of haemoglobin was measured in 101 patients en route from the operating room to the recovery room. Twenty-nine became significantly hypoxaemic during the journey. The incidence of hypoxaemia was not found to relate to age, weight, duration or type of surgery, type of anaesthesia or pre-existing disease. Duration of the interval between cessation of oxygen in the operating room and arrival in recovery room was the only significant finding in patients who became hypoxaemic.

Adolescent↗
PubMed1987

Mammographic and histopathologic correlation of nonpalpable lesions of the breast and the reliability of frozen section diagnosis.

During a period of ten years, 118 (32.9 per cent) instances of carcinoma were found in 359 specimens taken at biopsy for nonpalpable mammographic lesions. In recent years, the positive predictive value has increased from 68 per cent due to the development of magnification mammography and the use of a mammographic grid. Correlating mammographic and histopathologic data, the rate of malignant disease was 12.7 per cent for instances of a circumscribed or nodular mass, 32.4 per cent for clustered microcalcifications as the only suspect finding, 28.6 per cent when a mass with microcalcifications was present and 66.7 per cent when a stellate-shaped mass was found. Of 188 instances of carcinoma, 40 were noninvasive: 32 instances of ductal carcinoma (27.1 per cent) and eight of lobular carcinoma in situ (6.8 per cent). The possibility of frozen section diagnosis was studied retrospectively by comparison with the paraffin section reports. A correct diagnosis, whether benign or malignant, was achieved in 68 per cent. No frozen section examination was done in 17.3 per cent and the diagnosis was deferred to results of paraffin section in 12.2 per cent. False-negative results were encountered in seven patients (1.9 per cent) and false-positive results in two (0.6 per cent). Both of these patients had florid sclerosing adenosis. Although frozen section diagnosis is feasible in nonpalpable lesions of the breast, it is recommended that this method not be used in instances of pure microcalcifications and tiny solid masses of 5 millimeters or less.

Adult↗