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Biomedical subjects

R Hodges

Publications and source records attributed to R Hodges.

29 records · Page 2Linked to original sources
PubMed1981

Site of synthesis of the mitochondrial cytochromes in hepatocyte cultures.

The biosynthesis of mammalian mitochondrial cytochromes was explored in primary hepatocyte cultures. When these were pulsed with [35S]methionine in the presence of cycloheximide, eight discrete mitochondrial polypeptides were detected by fluorography after their resolution under denaturing conditions by polyacrylamide gel electrophoresis. Since the pulse labeling of the polypeptides was sensitive to chloramphenicol, an inhibitor of mitochondrial translation, they must be translated on mitochondrial ribosomes. Three were identified as the largest subunits of cytochrome oxidase by their immunoprecipitation with antibody directed against purified rat liver cytochrome oxidase. Another (Mr = 28,000) was identified as one of eight subunits of purified rat liver cytochrome b-c1 complex by its immunoprecipitation with antibody directed against bovine heart b-c1 complex. Since cytochrome b apoprotein is the only product of the mitochondrial genome in the yeast cytochrome b-c1 complex (Krieke, J., Bechmann, H., van Hemert, F. J., Schweyan, R. J., Boer, P. H., Kaudewitz, F., and Groot, G. S. P. (1979) Eur. J. Bio-chem. 101, 607-617), the results strongly suggest that the Mr = 28,000 subunit of liver b-c1 complex is cytochrome b apoprotein. Thus the contribution of the mitochondrial translation system to the cytochrome complexes in liver is identical to that of yeast and Neurospora, and there appears to be no deletion or transfer to the nuclear genome of structural genes for mitochondrially synthesized cytochromes during eukaryotic evolution.

Animals↗
PubMed1979

Intoxication of cells from different-aged embryos by diphtheria toxin.

Different methods were used to assay diphtheria toxin sensitivity of fibroblast and heart cell cultures from chicken embryos of various ages. As defined by inhibition of protein synthesis, fibroblasts from 18-day and younger embryos respond more rapidly to toxin than fibroblasts from older embryos. The response of heart cells cultures is independent of the age of the embryos and is similar to the response of fibroblasts from 18-day embryos. Since the EF-2 content is 10-fold less in fibroblasts from 21-day-old embryos, the different responses of protein synthesis to intoxication appear to reside at the membrane level. Cytotoxicity assays in cell culture and in vivo toxin sensitivity assays show that cells from both young and old embryos, as well as whole embryos, are equally sensitive to toxin. Thus, short-term (5-h) measurements of inhibition of protein synthesis are insufficient for determining the relative sensitivity of cells to intoxication as defined by cell death.

Animals↗
PubMedDate not supplied

Benchmarking and networking through collaborative groups.

The search for better ways to deliver healthcare services at lower cost led a group of hospitals in the Carolinas, 2 years ago, to pioneer a new concept, the collaborative group. A collaborative group typically is composed of five or six similar (but not competing) hospitals that work together closely to help each other make the changes necessary to survive in an increasingly competitive marketplace. Hospital staff members at all levels are able to work together to discover improvement opportunities through benchmarking teams and networking efforts. This article describes their experiences, achievements, and lessons learned.

Efficiency, Organizational↗
PubMedDate not supplied

Autonomy and competence as motivational factors in students with learning disabilities and emotional handicaps.

Over 450 students (136 elementary, 321 junior and senior high school) with primary handicapping codes of learning disability (LD) or emotional handicap (EH) completed several questionnaires. All participants were from self-contained classrooms of a state-operated special education system. Questionnaires assessed students' self-perceptions and perceptions of home and classroom contexts, with all variables theoretically reflecting either the competence or the autonomy aspects of internal motivation or students' personal adjustment. Math and reading standardized achievement test scores were obtained from school records. Using multiple regression analyses, students' achievement and adjustment were predicted from the motivationally relevant self-perception and perception-of-context variables. Interestingly, different patterns of relations emerged for the students with LD and EH.

Adaptation, Psychological↗